Summary
In the nerve tissue with proliferating macroglia cells were observed a lowered oxygen consumption, an increased aerobic glycolysis and alanine formation and a higher alanine aminotransferase and glutamate dehydrogenase activity than in the control tissue in the homogenates and in the cell sap fraction. The substrate saturation curves, apparent Km and pH optimum values in the tissue with proliferating macroglia and in the control did not differ from one another. The authors assume that a higher alanine amino-transferase activity in the tissue with macroglia proliferation can reflect either a higher synthesis of the enzyme in the altered tissue, or a predominance of glial elements in the altered tissue possessing a higher alanine aminotransferase activity than the nerve cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergmayer, H. U.: Methods of enzymatic analysis. New York-London: Acad. Press 1965
Friede, R. L.: The cytochemistry of normal and reactive astrocytes. J. Neuropath. exp. Neurol.21, 471–478 (1962)
Hager, H.: Allgemeine morphologische Pathologie des Nervengewebes. In: Handbuch der Allg. Pathologie, Vol. 3, part 3 (eds. H. W. Altmann, F. Büchner und H. Cottier) pp. 1–385. Berlin-Heidelberg-New York: Springer 1968
Hager, H., Luh, S., Ruščáková, D., Ruščák, M.: Histochemische, elektronenmikroskopische und biochemische Untersuchungen über Glykogenanhäufung in reaktiv veränderten Astrozyten der traumatisch lädierten Säugergroßhirnrinde. Z. Zellforsch.83, 295–312 (1967)
Katunuma, N., Okada, M., Katsunuma, T., Fujino, A., Matsuzawa, T.: Different metabolic roles of transaminase isoenzymes. In: Dynamic spatial aspects of enzymatic pyridoxal catalysis: Enzymes and model system (eds. E. E. Snell, A. E. Braunstein, E. S. Severin and Yu. M. Torchinskij), pp. 255–268. New York-London-Sydney: Intersci. Publ. 1968
Křivánek, J.: Spreading cortical depression and incorporation of14C from glucose into some amino acids and glycogen of the rat cerebral cortex. Physiol. bohemoslov.22, 77 (1973)
Lowry, O., Rosenbourgh, N., Farr, A., Randall, R.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951)
Miquel, J., Klatzo, J., Menzel, D. B., Haymaker, W.: Glycogen changes in x-irradiated rat brain. Acta neuropath. (Berl.)2, 482–491 (1963)
Orlický, J.: Porovnávacie štúdie o alaninaminotransferáze zo subcelulárnych štruktur CNS, pečene, srdcového svalu a kôry obličiek. Autoreferát dizertácie k získaniu hodnosti kandidáta chemických vied, Bratislava 1974
Orlický, J., Ruščák, M.: Comparison of mitochondrial and soluble alanine aminotransferase from various organs of the rat. Physiol. bohemoslov.22, 84 (1973)
Price, G. M.: The accumulation of α-alanine in the housfly Musca vicina. Biochem. J.81, 15 (1961)
Rose, S. P. R.: The biochemistry of neurons and glia. In: Applied Neurochemistry (eds. A. N. Davison and J. Dobbing), pp. 332–355. Oxford: Blackwell 1968
Rubinstein, L. J., Klatzo, J., Miquel, J.: Histochemical observations on oxidative enzyme activity of glial cells in a local brain injury. J. Neuropath. exp. Neurol.21, 116–136 (1962)
Ruščák, M., Orlický, J., Some properties of mitochondrial and soluble alanine aminotransferase from CNS of the rats. Physiol. bohemoslov.22, 91 (1973)
Ruščák, M., Orlický, J., Hager, H.: Alanine aminotransferase in the reactive and blastomatous macroglia. Physiol. bohemoslov.23, 373 (1974)
Ruščák, M., Ruščáková, D.: Metabolism of the nerve tissue in relation to ion movements in vitro and in situ. Baltimore-London-Tokyo: Univ. Park Press 1971
Shimada, M., Kihara, T., Kurimoto, K., Watanabe, M.: Incorporation of14C from (U-14C) glucose into free amino acids in mouse brain loci in vivo under normal conditions. J. Neurochem.20, 1337–1344 (1973)
Shimizu, N., Hamuro, I.: Deposition of glycogen and changes in some enzymes in brain wounds. Nature (Lond.)181, 781 (1958)
Yoshino, Y., Elliot, K. A. C.: Incorporation of carbon atoms from glucose into free amino acids in brain under normal and altered conditions. Canad. J. Biochem.48, 228–235 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ruščák, M., Hager, H. & Orlický, J. Alanine formation and alanine aminotransferase activity in the nerve tissue with proliferating macroglia. Acta Neuropathol 34, 149–155 (1976). https://doi.org/10.1007/BF00684665
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00684665