Summary
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1.
Sodium fluorescein and Evans Blue, commonly used tracers in the study of blood-brain barrier disturbances, revealed considerable differences in their respective protein binding capacity in the plasma, passage through the barrier and in the rate of their elimination from the brain parenchyma.
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2.
In the plasma a considerable portion of the sodium fluorescein remains free and behaves like a micromolecular barrier tracer. On the other hand, almost complete binding of the Evans Blue to albumin confers to it properties of a protein tracer.
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3.
Following the extravasation of the tracers, the sodium fluorescein is relatively soon eliminated, whereas Evans Blue remains in the cellular elements of the brain parenchyma for a considerable time, although the protein moiety of the tracer is removed much sooner from the cytoplasm of glial cells, presumably by the lysosomal digestion.
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Tel Aviv University Sackler School of Medicine and the Ch. Sheba Medical Center, Israel
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Wolman, M., Klatzo, I., Chui, E. et al. Evaluation of the dye-protein tracers in pathophysiology of the blood-brain barrier. Acta Neuropathol 54, 55–61 (1981). https://doi.org/10.1007/BF00691332
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DOI: https://doi.org/10.1007/BF00691332