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Immunohistochemical demonstration of carbonic anhydrase III and muscle-specific enolase in paraffin-embedded human skeletal muscle sections

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Summary

We have demonstrated the histochemical fiber types of human skeletal muscle in paraffin sections by the immunohistochemical method, together with compatible observations previously made in frozen sections that carbonic anhydrase III is mainly localized in type 1 fibers (Shima et al. 1983), and muscle-specific enolase in type 2 fibers (Ibi et al. 1983). This method is useful to analyze the fiber types when frozen muscle samples at biopsy or autopsy cannot be obtained.

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Authors and Affiliations

  1. Dept. IV of Internal Medicine, Division of Neurology, Aichi Medical University, Nagakute-cho, Aichi-gun, 480-11, Aichi, Japan

    T. Ibi & K. Sahashi

  2. Dept. I of Pathology, Nagoya University School of Medicine, Aichi, Japan

    H. Haimoto

  3. Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Aichi, Japan

    H. Nagura

  4. Dept. of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, Aichi, Japan

    K. Kato

  5. Dept. I of Internal Medicine, Nagoya University School of Medicine, Aichi, Japan

    K. Mokuno, K. Sugimura & Y. Matsuoka

Authors
  1. T. Ibi
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  2. H. Haimoto
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  3. H. Nagura
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  4. K. Sahashi
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  5. K. Kato
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  6. K. Mokuno
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  7. K. Sugimura
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  8. Y. Matsuoka
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Ibi, T., Haimoto, H., Nagura, H. et al. Immunohistochemical demonstration of carbonic anhydrase III and muscle-specific enolase in paraffin-embedded human skeletal muscle sections. Acta Neuropathol 68, 74–76 (1985). https://doi.org/10.1007/BF00688959

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  • DOI: https://doi.org/10.1007/BF00688959

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