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An immunohistochemical study of glial and neuronal markers in primary neoplasms of the central nervous system

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Summary

Paraffin-embedded tissues from 56 primary neoplasms of the central nervous system and seven cases of non-neoplastic reactive astrocytosis were examined by immunoperoxidase techniques on serial sections using monoclonal antibodies to glial fibrillary acidic protein (GFAP) and the 68 kDa neurofilament subunit and monospecific polyclonal antibodies to α-and γ-enolase. γ-Enolase was present in all neoplasms of neuronal origin, but was also present in anaplastic gliomas (particularly in giant cells), in some well-differentiated astrocytomas and reactive astrocytes. The cells containing γ-enolase in these cases appeared morphologically identical to those containing α-enolase and GFAP in adjacent serial sections. No relationship was found between γ-enolase immunoreactivity and cellular anaplasia in the gliomas studied. Subependymal neoplasms from patients with tuberose sclerosis exhibited evidence of both astrocytic and neuronal differentiation, sometimes in morphologically distinct cell populations, consistent with their suggested origin from a primitive cell line.

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References

  1. Beemer FA, Vlug AMC, Cees WM, van Veelen M, Rijksen G, Staal GEJ (1984) Isozyme pattern of enolase of childhood tumors. Cancer 54:293–296

    Google Scholar 

  2. Bender BL, Yunis EJ (1980) Central nervous system pathology of tuberous sclerosis in children. Ultrastruct Pathol 1:287–299

    Google Scholar 

  3. Bonnin JM, Rubinstein LJ (1984) Immunohistochemistry of central nervous system tumors: its contributions to neurosurgical diagnosis. J Neurosurg 60:1121–1133

    Google Scholar 

  4. Bonnin JM, Rubinstein LJ, Papasozomenos SCh, Marangos PJ (1984) Subependymal giant cell astrocytoma. Significance and possible cytogenetic implications of an immunohistochemical study. Acta Neuropathol (Berl) 62:185–193

    Google Scholar 

  5. Carlei F, Polak JM, Ceccamea A, Marangos PJ, Dahl D, Cocchia D, Michetti F, Lezoche E, Speranza V (1984) Neuronal and glial markers in tumors of neuroblastic origin. Virchows Arch [A] 404:313–324

    Google Scholar 

  6. Duffy PE, Graf L, Huang Y-Y, Rapport MM (1979) Glial fibrillary acidic protein in ependymonas and other brain tumors. J Neurol Sci 40:133–146

    Google Scholar 

  7. Duffy PE, Huang Y-Y, Rapport MM, Graf L (1980) Glial fibrillary acidic protein in giant cell tumors of brain and other gliomas. Acta Neuropathol (Berl) 52:51–57

    Google Scholar 

  8. Graham DI, Thomas DGT, Brown I (1983) Invited review: nervous system antigens. Histopathology 7:1–21

    Google Scholar 

  9. Haglid KG, Carlsson CA, Stavrou D (1973) An immunological study of human brain tumours concerning the brainspecific proteins S-100 and 14-3-2. Acta Neuropathol (Berl) 24:187–196

    Google Scholar 

  10. Herpers MJH, Budka H (1984) Glial fibrillary acidic protein (GFAP) in oligodendroglial tumors: gliofibrillary oligodendroglioma and transitional oligoastrocytoma as subtypes of oligodendroglioma. Acta Neuropathol (Berl) 64:265–272

    Google Scholar 

  11. Kluch P, van Juijen GNP, van der Kamp AWM, Tibboel D, van Hoorn WA, Warnaar SO, Molenaar JC (1984) Hirschprung's disease studied with monoclonal antineurofilament antibodies on tissue sections. Lancet I:652–654

    Google Scholar 

  12. Nakamura Y, Becker LE (1983) Subependymal giant cell tumor: astrocytic or neuronal? Acta Neuropathol (Berl) 60:271–277

    Google Scholar 

  13. Pachter JS, Liem RKH (1984) The differential appearances of neurofilament triplet polypeptides in the developing rat optic nerve. Dev Biol 103:200–210

    Google Scholar 

  14. Raff MC, Miller RH (1984) Glial cell development in the rat optic nerve. Trend Neurosci 7:469–472

    Google Scholar 

  15. Roessman U, Velasco ME, Gambetti P, Autilio-Gambetti L (1983) Neuromal and astrocytic differentiation in human neuroepithelial neoplasms: an immunohistochemical study. J Neuropathol Exp Neurol 42:113–121

    Google Scholar 

  16. Royds JA, Parsons MA, Taylor CB, Timperley MR (1982) Enolase isoenzyme distributien in the human brain and its tumours. J Pathol 137:37–49

    Google Scholar 

  17. Rubinstein LJ (1982) Diagnostic applicability of GFA protein immunoperoxidase staining. Tumors of the central nervous system, [Suppl] Fascicle 6, Second Series in the Atlas of Tumor Pathology, Armed Forees Institute of Pathology, Washington DC, pp S21-S30

    Google Scholar 

  18. Sasaki A, Ogawa A, Nakazato Y, Ishida Y (1985) Distribution of neurofilament protein and neuron-specific enolase in peripheral neuronal tumors. Virchows Arch [A] 407:33–41

    Google Scholar 

  19. Schmechel D, Marangos PJ, Zis AP, Brightman M, Goodwin FK (1978) The brain enolases as markers of neuronal and glial cells. Science 199:313–315

    Google Scholar 

  20. Shaw G, Weber K (1982) Differential expression of neurofilament triplet proteins in brain development. Nature 298:277–279

    Google Scholar 

  21. Stefansson K, Wollmann R (1980) Distribution of glial fibrillary acidic protein in central nervous system lesions of tuberose sclerosis. Acta Neuropathol (Berl) 52:135–140

    Google Scholar 

  22. Stefansson K, Wollmann R (1981) Distribution of the neuronal specific protein 14-3-2 in central nervous system lesions of tuberous sclerosis. Acta Neuropathol (Berl) 53:113–117

    Google Scholar 

  23. Sternberger LA (1979) Immunocytochemistry, 2nd edn. John Wiley and Sons, New York

    Google Scholar 

  24. Trojanowski JQ, Lee VM-Y (1983a) Monoclonal and polyclonal antibodies against neural antigens: diagnostic applications for studies of central and peripheral neural system tumors. Hum Pathol 14:281–285

    Google Scholar 

  25. Trojanowski JQ, Lee VM-Y (1983b) Anti-neurofilament monoclonal antibodies: reagents for the evaluation of human neoplasms. Acta Neuropathol (Berl) 59:155–158

    Google Scholar 

  26. Trombley IK, Mirra SS (1981) Ultrastructure of tuberous sclerosis: cortical tuber and sunependymal tumor. Ann Neurol 9:174–181

    Google Scholar 

  27. Velasco ME, Ghobrial MW, Ross ER (1985) Neuronspecific enolase and neurofilament protein as markers of differentiation in medulloblastoma. Surg Neurol 23:177–182

    Google Scholar 

  28. Vinores SA, Bonnin JM, Rubinstein LJ, Marangos PJ (1984) Immunohistochemical demonstration of neuron-specific enolase in neoplasms of the CNS and other tissues. Arch Pathol Lab Med 108:536–540

    Google Scholar 

  29. Vinores SA, Rubinstein LJ (1985) Simultaneous expression of glial fibrillary acidic (GFA) protein and neuron-specific enolase (NSE) by the same reactive and neoplastic astrocytes. Neuropathol Appl Neurobiol 11:349–359

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Sheffield, Western Bank, S10 2TN, Sheffield, UK

    J. A. Royds & C. B. Taylor

  2. Department of Neuropathology, Floor N, Royal Hallamshire Hospital, Glossop Road, S10 2JF, Sheffield, South Yorkshire, UK

    J. W. Ironside & W. R. Timperley

  3. Department of Neuropathology, Institute of Neurological Sciences, Southern General Hospital, G51 4TF, Glasgow, UK

    D. I. Graham

Authors
  1. J. A. Royds
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  2. J. W. Ironside
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  3. C. B. Taylor
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  4. D. I. Graham
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  5. W. R. Timperley
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Supported by Wellcome Trust Grant No. 11353/1.5

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Royds, J.A., Ironside, J.W., Taylor, C.B. et al. An immunohistochemical study of glial and neuronal markers in primary neoplasms of the central nervous system. Acta Neuropathol 70, 320–326 (1986). https://doi.org/10.1007/BF00686091

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  • DOI: https://doi.org/10.1007/BF00686091

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