Summary
Alzheimer's neurofibrillary tangles (ANT) in the hippocampal area were studied immunohistochemically using antisera against glial fibrillary acidic protein (GFAP) and S-100 protein in 48 patients with or without dementia between 52 and 92 years old. In 27 of the 38 brains that developed ANT in the hippocampal area, some ANT were immunostained with these antisera. Flame-shaped or globose-shaped immunostains were occasionally continuous with astroglial cell bodies and processes. They appeared particularly in the entorhinal cortex, subiculum and CAl. The ANT, immunostained with GFAP and S-100 antisera, apparently correspond to slightly eosinophilic tangles in H&E sections and to less argentophilic tangles in silver-impregnated sections in all of the 27 brains. ANT of another 11 brains were consistently negative with these antisera. The GFAP-positive eosinophilic tangles were encountered in the brains of older patients (P<0.01) and with more abundant formation of ANT (P<0.001). This alteration was present in all of the 20 brains with more than 100 ANT per section and none of the eight brains with less than 10 ANT. These findings suggest that in the last stages, ANT are penetrated by eosinophilic processes of astrocytes, and appear eosinophilic, and that the presence of GFAP-positive eosinophilic tangles indicates the abundant formation of ANT.
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References
Alzheimer A (1911) Über eigenartige Krankheitsfälle des späteren Alters. Z Gesamte Neurol Psychiatr 4:356–385
Braak H, Braak E (1985) On areas of transition between entorhinal allocortex and temporal isocortex in the human brain. Normal morphology and lamina-specific pathology in Alzheimer's disease. Acta Neuropathol (Berl) 68:325–332
Hirano A (1981) A guide to neuropathology. Igaku-Shoin, New York Tokyo, pp 144–145
Hirano A (1976) An outline of neuropathology, 1st edn. Igaku-Shoin, Tokyo, pp 54–55
Hyman BT, Hoesen GW van, Damasio AR, Barnes CL (1984) Alzheimer's disease: cell-specific pathology isolates the hippocampal formation. Science 225:1168–1170
Morimatsu M, Hirai S, Muramatsu A, Yoshikawa M (1975) Senile degenerative brain lesions and dementia. J Am Geriatr Soc 23:390–406
Nakazato Y, Ishizeki J, Takahashi T, Yamaguchi H, Kamei T, Mori T (1982) Localization of S-100 protein and glial fibrillary acidic protein-related antigen in pleomorphic adenoma of the salivary glands. Lab Invest 46:621–626
Okamoto K, Hirano A, Yamaguchi H, Hirai S (1983) The fine structure of eosinophilic stages of Alzheimer's neurofibrillary tangles. J Clin Electron Microsc 16:77–82
Probst A, Ulrich J, Heitz Phu (1982) Senile dementia of Alzheimer type: a astroglial reaction to extracellular neurofibrillary tangles in the hippocampus. An immunocytochemical and electron microscopic study. Acta Neuropathol (Berl) 57:75–79
Sternberger LA (1979) Immunohistochemistry, 2nd edn. Wiley, New York Chichester Brisbane Tronto, pp 129–172
Takahashi K, Yamaguchi H, Ishizeki, J, Nakajima T, Nakazato Y (1981) Immunohistochemical and immunoelectron microscopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node. Virchows Arch [Cell Pathol] 37:125–135
Yamaguchi H (1980) Studies on the immunohistochemical localization of S-100 and glial fibrillary acidic proteins in the rat nervous system and in human brain tumors. Brain Nerve (Tokyo) 32:1055–1064
Yamaguchi H, Takahashi K, Morimatsu M, Okamoto K, Hirai S (1981) Immunohistochemical studies on senile dementia. Abstr 12th World Congr Neurol, Int Congr Series 548, Excerpta Medica, pp 79–80
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Yamaguchi, H., Morimatsu, M., Hirai, S. et al. Alzheimer's neurofibrillary tangles are penetrated by astroglial processes and appear eosinophilic in their final stages. Acta Neuropathol 72, 214–217 (1987). https://doi.org/10.1007/BF00691092
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DOI: https://doi.org/10.1007/BF00691092