Summary
Against the background of the importance of growth hormone (GH) for normal muscle growth, a study was performed to investigate whether lack of GH after hypophysectomy affects the cell proliferation and the local production of insulin-like growth factor-I (IGF-I) in the early stages of muscle regeneration in adult rats. The level of IGF-I in the serum of hypophysectomised rats was reduced to about 30% of that of controls. The incorporation of [methyl-3H]thymidine into the regenerating muscle showed a peak 6 days after the operation and then gradually declined to the end of the period of study 30 days after initiation of regeneration by ischemic necrosis. The DNA content rose to a maximum level after 6–8 days, and remained high after 30 days. There was no major difference in the incorporation of [3H]thymidine in regenerating muscle of hypophysectomised and control rats, but the DNA concentration in the regenerating muscles of hypophysectomised rats was significantly reduced after 30 days. There was a corresponding reduction in the number of nuclei per muscle fibre, indicating that hypophysectomy has a small effect on the cell proliferation during the early stages of muscle regeneration. Immunohistochemical demonstration of IGF-I in the regenerating muscle revealed the transient presence of immunoreactive material in satellite cells and myotubes after 6 to 8 days of regeneration but no immunoreactivity after 30 days. No obvious difference was observed between hypophysectomised and control rats, indicating that the endogenous production of IGF-I in regenerating skeletal muscle can occur independently of GH.
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References
Ballard FJ, Read LC, Francis GL, Bagley CJ, Wallace JC (1986) Binding properties and biological potencies of insulin-like growth facors in L6 myoblasts. Biochem J 233:223–230
Carlson BM, Gutmann E (1975) Regeneration in free grafts of normal and denervated muscles in the rat: morphology and histochemistry. Anat Rec 183:47–62
Cheek DB, Graystone JE (1969) The action of insulin, growth hormone, and epinephrine on cell growth in liver, muscle, and brain of the hypophysectomised rat. Pediatr Res 3:77–88
Church JCT, Noronha RFX, Allbrook DB (1966) Satellite cells and skeletal muscle regeneration. Br J Surg 53:638–642
Daughaday W, Mariz I, Blethen S (1980) Inhibition of access of bound somatomedin to membrane receptor and immunobinding sites: a comparison of radioreceptor and radioimmunoassay of somatomedin in native and acid-ethanol-extracted serum. J Clin Endocrinol Metab 51:781–788
Ewton DZ, Falen SL, Florini JR, Van Wyk JJ (1987) Low affinity of type II receptors for the Thr 59 IGF-I analog. pleiotypic actions of IGFs on myoblasts are apparently mediated by the type I receptor. Endocrinology 120:115–123
Fischman DA (1986) Myofibrillogenesis and the morphogenesis of skeletal muscle. In: Engel A, Banker B (eds) Myology. McGraw-Hill, New York, pp 5–37
Flaim KE, Li BJ, Jefferson LS (1978) Protein turnover in rat skeletal muscle: effects of hypophysectomy and growth hormone. Am J Physiol 234:E38-E43
Florini JR (1987) Hormonal control of muscle growth. Muscle Nerve 10:577–598
Florini JR, Ewton DZ, Evinger-Hodges MJ, Falen SL, Lau RL, Regan JF, Vertel BM (1984) Stimulation and inhibition of myobalast differentiation by hormones. In Vitro 20:942–958
Florini JR, Ewton DZ, Falen SL, Van Wyk JJ (1986) Biphasic concentration dependency of stimulation of myoblast differentiation by somatomedins. Am J Physiol 250:C771-C778
Goldberg AL, Tischler M, DeMartinio G, Griffin G (1980) Hormonal regulation of protein degradation and synthesis in skeletal muscle. Fed Proc 39:31–36
Herington AC, Cornell HJ, Kuffer AD (1983) Recent advances in the biochemistry and physiology of the insulin-like growth factor/somatomedin family. Int J Biochem 15:1201–1210
Isaksson OGP, Edén S, Jansson J-O (1985) Mode of action of pituitary growth hormone on target cells. Annu Rev Physiol 47:483–499
Jennische E, Skottner A, Hansson H-A (1987) Satellite cells express the trophic factor IGF-I in regenerating skeletal muscle. Acta Physiol Scand 129:9–15
Jirmanová I, Thesleff S (1972) Ultrastructural study of experimental muscle degeneration and regeneration in the adult rat. Z Zellforsch Mikrosk Anat 131:77–97
Jones PH (1982) In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult skeletal muscle. Exp Cell Res 139:401–404
Kelly AM (1978) Satellite cells and myofibre growth in the rat soleus and extensor digitorum longus muscles. Dev Biol 65:1–10
Kissane JM, Robins E (1958) The fluorometric measurement of deoxiribonucleic acid in animal tissue with special reference to the central nervous system. J Biol Chem 233: 184–188
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Mazanet R, Franzini-Armstrong C (1986) The satellite cell. In: Engel A, Banker B (eds) Myology. McGraw-Hill, New York, pp 285–307
Murphy LJ, Bell GI, Duckworth ML, Friesen HG (1987) Identification, characterization, and regulation of a rat complementary deoxiribonucleic acid which encodes insulin-like growth factor-I. Endocrinology 121:684–691
Ontell M (1974) Muscle satellite cells: a validated technique for light microscopic identification and quantitative study of changes in their population following denervation. Anat Rec 178:211–228
Phillips LS, Unterman TG (1984) Somatomedin activity in disorders of nutrition and metabolism. Clin Endocrinol Metab 13:145–189
Schoenle E, Zapf J, Humbel RE, Froesch ER (1982) Insulin-like growth factor I stimulates growth in hypophysectomised rats. Nature 296:252–253
Schultz E (1984) A quantitative study of satellite cells in regenerated soleus and extensor digitorum longus muscles. Anat Rec 208:501–506
Schwartz J (1982) Rapid modulation of protein synthesis in normal rats by specific neutralization and replacement of growth hormone. Endocrinology 111:2087–2090
Shafiq S, Gorycki MA (1965) Regeneration of skeletal muscle of mouse. Some electron microscopic observations. J Pathol Bacteriol 90:123–127
Snow MH (1978) An autoradiographic study of satellite cell differentiation into regnerating myotubes following transplantation of muscles in young rats. Cell Tiss Res 186:535–540
Walker DG, Simpson ME, Asling CW, Evans HM (1950) Growth and differentiation in the rat following hypophysectomy at 6 days of age. Anat Rec 106:539–554
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Supported by the Swedish Medical Research Council (Project no. 7122 and 7120)
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Sommerland, H., Ullman, M., Jennische, E. et al. Muscle regeneration. Acta Neuropathol 78, 264–269 (1989). https://doi.org/10.1007/BF00687756
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DOI: https://doi.org/10.1007/BF00687756