Summary
A well-defined coagulative lesion was produced in the fronto-parietal cortex of adult rats by application of a cold probe, and the chronological sequence of events during the healing process, particularly the relationship between astroglial processes and the newly forming basal lamina (BL) and the behavior of the extracellular matrix (ECM) was examined immunocytochemically and ultrastructurally. By electron microscopy, new BL synthesis was first noted 7 days following injury, and a continuous and well-defined BL was present from 14 days onward. These findings correlated well with the pattern of immunoreactivity for laminin and for type IV collagen. Both laminin and type IV collagen appeared both to be produced primarily by mesenchymal cells within and around the wound as well as those of the blood vessels, and to become an integral part of the new BL. Although there was no immunocytochemical evidence to indicate secretion of laminin, type IV collagen or fibronectin by astrocytes, a well-defined BL appeared to form only in tight apposition with astroglial processes over the pial surface. This suggests that the BL is formed by subpial astrocytes in close interaction with ECM components at the pial surface. Fibronectin appears to contribute significantly to the formation of the BL by providing a suitable substratum for the coordinated cellular interaction necessary for successful regeneration of the BL.
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References
Bernstein JJ, Getz R, Jefferson M, Kelemen M (1985) Astrocytes secrete basal lamina after hemisection of rat spinal cord. Brain Res 327:135–141
Berry M, Maxwell WL, Logan A, Mathewson A, McConnell P, Ashhust DE, Thomas GH (1983) Deposition of scar tissue in the central nervous system. Acta Neurochir [Suppl] (Wien) 32:31–53
Carbonell AL, Boya J (1988) Ultrastructural study on meningeal regeneration and meningo-glial relationships after cerebral stab wound in the adult rat. Brain Res 439:337–344
Choi BH (1988) Developmental events during the early stages of cerebral cortical neurogenesis in man. A correlative light, electron microscopic, immunohistochemical and Golgi study. Acta Neuropathol (Berl) 75:441–447
Choi BH (1988) Prenatal gliogenesis in the developing cerebrum of the mouse. Glia 1:308–316
Choi BH, Matthias S (1987) Cortical dysplasia associated with massive ectopia of neurons and glial cells within the subarachnoid space. Acta Neuropathol (Berl) 73:105–109
Feringa ER, Vahlsing HL, Woodward M (1984) Basal lamina at the site of spinal cord transection in the rat: an ultrastructural study. Neuroscience Lett 51:303–308
Furthmayr H (1988) Basement membranes. In: Clark RAF, Henson PM (eds) The molecular and cellular biology of wound repair. Plenum Press, New York, pp 525–558
Hynes RO (1986) Fibronectins. Sci Am 254:42–51
Kusaka H, Hirano A, Bornstein MB, Raine CS (1985) Basal lamina formation by astrocytes in organotypic cultures of mouse spinal cord tissue. J Neuropathol Exp Neurol 44:295–303
Leblond CP, Inoue S (1989) Structure, composition, and assembly of basement membrane. Am J Anat 185:367–390
Liesi P, Risteli L (1989) Glial cells of mammalian brain produce a variant form of laminin. Exp Neurol 105:86–92
Liesi P, Dahl D, Vaheri A (1984) Laminin is produced by early rat astrocytes in primary culture. J Cell Biol 96:920–924
Liesi P, Kaakkola S, Dahl D, Vaheri A (1984) Laminin is induced in astrocytes of adult brain by injury. EMBO J 3:683–686
Lyser KM (1972) The differentiation of glial cells and glia limitans in organ cultures of chick spinal cord. In vitro 8:77–84
Madri JA, Pratt BM, Yurchenco PD, Furthmayr H (1988) The ultrastructural organization and architecture of basement membranes.
Martinez-Hernandez A, Amenta PS (1983) The basement membrane in pathology. Lab Invest 48:656–677
Maxwell WL, Duance VC, Letho M, Ashurst DE, Berry M (1984) The distribution of types I, III, IV collagens in penetrant lesions of the central nervous system of the rat. Histochem J 16:1219–1229
Merker HJ (1987) Production and formation of the basal lamina. In: Wolff JR, Sievers J, Berry M (eds) Mesenchymalepithelial interactions in neural development. Springer, Berlin Heidelberg, pp 53–63
Price J, Hynes RO (1985) Astrocytes in culture synthesize and secrete a variant form of fibronectin. J Neurosci 5:2205–2211
Rutka JT, Giblin J, Dougherty DV, McCulloch JR, DeArmond SJ, Rosenblum ML (1986) An ultrastructural and immunocytochemical analysis of leptomeningeal and meningioma cultures. J Neuropathol Exp Neurol 45:285–303
Rutka JT, Apodaca G, Stern R, Rosenblum M (1988) The extracellular matrix of the central and peripheral nervous systems: structure and function. J Neurosurg 69:155–170
Sanderson RD, Fitch JM, Linsenmayer TR, Mayne R (1986) Fibroblasts promote the formation of a continuous basal lamina during myogenesis in vitro. J Cell Biol 102:740–747
Sanes JR (1983) Roles of extracellular matrix in neural development. Annu Rev Physiol 45:581–600
Timple R, Rohde H (1979) Laminin — A glycoprotein from basement membranes. J Biol Chem 254:9933–9937
Scott PG (1982) Macromolecular constituents of basement membranes: a review of current knowledge on their structure and function. Can J Biochem Cell Biol 61:942–948
Vracko R (1974) Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. A review. Am J Pathol 77:314–347
Yurchenco PD, Tsilibary EC, Charonis AS, Furthmayr H (1986) Models for the self-assembly of basement membrane. J Histochem Cytochem 34:93–102
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Supported in part by NIEHS grant ES 02928
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Suzuki, M., Choi, B.H. The behavior of the extracellular matrix and the basal lamina during the repair of cryogenic injury in the adult rat cerebral cortex. Acta Neuropathol 80, 355–361 (1990). https://doi.org/10.1007/BF00307687
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DOI: https://doi.org/10.1007/BF00307687