Summary
The ultrastructure of the curly fibers was examined by the transmission and immunoelectron microscopy as well as by the rapid-freeze, deep-etch and replica method. The curly fibers consisted mainly of paired helical filaments (PHF) in the neuropils, both pre- and post-synaptic. On the deep-etch replicas, PHF in the neurites had similar dimensions to those of Alzheimer's neurofibrillary tangles in the nerve cell somata, having the width of 28 to 36 nm. The two component filaments, 14 to 18 nm in width, were twisted left-handedly with about 70- to 90-nm intervals. There were also cross-bridging fibrils of about 6 nm in diameter connecting the neighboring PHF.
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References
Barcikowska M, Wisniewski HM, Bancher C, Grundke-Iqbal I (1989) About the presence of paired helical filaments in dystrophic neurites participating in the plaque formation. Acta Neuropathol 78:225–231
Braak H, Braak E, Grundke-Iqbal I, Iqbal K (1986) Occurrence of neuropil threads in the senile human brain and in Alzheimer's disease: a third location of paired helical filaments outside of neurofibrillary tangles and neuritic plaques. Neurosci Lett 65:351–355
Dickson DW, Farlo J, Davies P, Crystal H, Fuld P, Yen S-Hc (1988) Alzheimer's disease. A double-labeling immunohistochemical study of senile plaques. Am J Pathol 132:86–101
Ihara Y (1988) Massive somatodendritic sprouting of cortical neurons in Alzheimer's disease. Brain Res 459:138–144
Ihara Y, Abraham C, Selkoe DJ (1983) Antibodies to paired helical filaments in Alzheimer's disease do not recognize normal brain proteins. Nature 304:727–730
Kidd M (1964) Alzheimer's disease: an electron microscopical study. Brain 87:307–320
Kosik KS, Joachim CL, Selkoe DJ (1986) Microtubule-associated protein tau is a major antigenic component of paired filaments in Alzheimer disease. Proc Natl Acad Sci USA 83:4044–4048
Kowall NW, Kosik KS (1987) Axonal disruption and aberrant localization of tau protein characterized the neuropil pathology of Alzheimer's disease. Ann Neurol 22:639–643
Kuzuhara S, Mori H, Izumiyama N, Yoshimure M, Ihara Y (1988) Lewy bodies are ubiquitinated. A light and electron microscopic immunocytochemical study. Acta Neuropathol (Berl) 75:345–353
Miyakawa T, Katsuragi S, Araki K, Hashimure T, Kimura T (1989) Ultrastructure of neurofibrillary tangles in Alzheimer's disease. Virchows Arch [B] 57:267–273
Nukina N, Ihara Y (1986) One of the antigenic determinants of paired helical filaments is related to tau protein. J Biochem (Tokyo) 99:1541–1544
Ohtsubo K, Izumiyama N, Shimada H, Tachikawa T, Nakamura H (1990) Three-dimensional structure of Alzheimer's neurofibrillary tangles of the aged human brain revealed by the quick-freeze, deep-etch and replica method. Acta Neuropathol 79:480–485
Papasozomenos SC (1989) Tau protein immunoreactivity in dementia of the Alzheimer type. II. Electron microscopy and pathogenetic implications. Effects of fixation on the morphology of the Alzheimer's abnormal filaments. Lab Invest 60:375–389
Probst A, Basler V, Brion B, Ulrich J (1983) Neuritic plaques in senile dementia of Alzheimer type: a Golgi analysis in the hippocampal region. Brain Res 268:249–254
Probst A, Anderton BH, Brion J-P, Ulrich J (1989) Senile plaque neurites fail to demonstrate anti-paired helical filament and anti-microtubule-associated protein-tau immunoreactive proteins in the absence of neurofibrillary tangles in the neocortex. Acta Neuropathol 77:430–436
Selkoe DJ (1989) Biochemistry of altered brain proteins in Alzheimer's disease. Annu Rev Neurosci 12:463–490
Terry RD (1963) The fine structure of neurofibrillaryl tangles in Alzheimer's disease. J Neuropathol Exp Neurol 22:629–642
Terry RD (1985) Alzheimer's disease. In: Davis RL, Robertson DM (eds) Textbook of neuropathology. Williams and Wilkins, Baltimore London Sydney, pp 824–841
Wisniewski HM, Wen GY (1985) Substructures of paired helical filaments from Alzheimer's disease neurofibrillary tangles. Acta Neuropathol (Berl) 66:173–176
Wisniewski HM, Sinatra RS, Iqbal K, Grundke-Iqbal I (1981) Neurofibrillary and synaptic pathology in the aged brain. In: Johnson JE (ed) Aging and cell structure, vol 1. Plenum Press, New York London, pp 105–142
Wolozin B, Davies P (1987) Alzheimer-related neuronal protein A68: specificity and distribution. Ann Neurol 22:521–526
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Ohtsubo, K., Izumiyama, N., Kuzuhara, S. et al. Curly fibers are tau-positive strands in the pre- and post-synaptic neurites, consisting of paired helical filaments: observations by the freeze-etch and replica method. Acta Neuropathol 81, 111–115 (1990). https://doi.org/10.1007/BF00334498
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DOI: https://doi.org/10.1007/BF00334498