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The effects of chronic serum sickness on albumin distribution and glucose utilization in rat brain

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Summary

The level of cerebrospinal fluid (CSF) protein is elevated in diseases and disease models that are associated with circulating immune complexes such as serum sickness. Circulatory immune complexes are known to deposit in the basal lamina of fenestrated capillaries and may, as a result, affect both capillary bed and parenchymal function. Since the brain has both fenestrated and unfenestrated capillaries and immune complexes deposit to a varying extent in the fenestrated capillaries in chronic serum sickness, cerebral capillary permeability to protein may be altered in some brain areas and lead to the elevation of CSF proteins. In addition various other cerebrovascular and metabolic functions may also be affected by this condition. In this study either radio-iodinated serum albumin (RISA) or 2-[14C]deoxyglucose (14C-2DG) was intravenously injected into control Wistar rats and Wistar rats with chronic serum sickness; subsequently the tissue levels of radioactivity were measured by quantitative autoradiography in 4 brain areas with fenestrated capillaries and 11 brain areas with unfenestrated capillaries. The 2-min distribution of RISA, which demarcates the volume of circulating plasma in perfused microvessels and is generally proportional to local plasma flow, was the same in control and experimental rats. The passage of RISA from blood into brain over 30 min was negligible in both groups; thus cerebral capillary permeability to albumin was not detectably increased in any of these 15 brain areas by chronic serum sickness. The rate of local cerebral glucose utilization, an indicator of local metabolic and neural activity, was calculated from the 14C-2DG data and was virtually identical in control and experimental rats. These results suggest that chronic serum sickness at this stage has little effect on capillary bed permeability and parenchymal function in most, if not all, brain areas.

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References

  1. Arisz L, Noble B, Milgrom M, Brentjens JR, Andres GA (1979) Experimental chronic serum sickness in rats. A model of immune complex glomerulonephritis and systemic immune complex disease deposition. Int Arch Allergy Appl Immunol 60:80–88

    Google Scholar 

  2. Aronson AJ, Ordonez NG, Diddie KR, Ernest TJ (1979) Immune complex deposition in the eye in systemic lupus erythematosus. Arch Intern Med 139:1312–1313

    Google Scholar 

  3. Atkins CJ, Kondon JJ, Quismorio FP, Friou GJ (1972) The chroid plexus in systemic lupus erythematosus. Ann Intern Med 76:65–72

    Google Scholar 

  4. Blasberg RG, Groothuis DR, Molnar P (1981) The application of quantitative autoradiographic measurements in experimental brain tumors. Semin Neurol 1:203–223

    Google Scholar 

  5. Blasberg RG, Fenstermacher JD, Patlak CS (1983) Transport of alpha-aminoisobutyric acid across the brain capillary and cellular membranes. J Cereb Blood Flow Metab 3:8–32

    Google Scholar 

  6. Blasberg R, Molnar P, Groothuis D, Patlak C, Owens E, Fenstermacher J (1984) Concurrent measurements of blood flow and transcapillary transport in avian sarcoma virus-induced experimental brain tumors: implications for chemotherapy. J Pharmacol Exp Ther 231:724–735

    Google Scholar 

  7. Bryan RM, Myers CL, Page RB (1988) Regional neurohypophyseal and hypothalamic blood flow in rats during hypercapnia. Am J Physiol 255:R295-R302

    Google Scholar 

  8. Chin WT, Kroontje W (1961) Conductivity method for determination of urea. Anal Chem 33:1757–1760

    Google Scholar 

  9. Cochrane CG, Kofflet D (1973) Immune complex disease in experimental animals and man. Adv Immunol 16:185–264

    Google Scholar 

  10. Cremer JE, Cunningham VJ, Seville MP (1983) Relationships between extraction and metabolism of glucose, blood flow, and tissue blood volume in regions of rat brain. J Cereb Blood Flow Metab 3:291–302

    Google Scholar 

  11. Dernouchamps JP, Vaerman JP, Michiels J, Masson PL (1977) Immune complexes in the aqueous humor and serum. Am J Ophthalmol 84:24–31

    Google Scholar 

  12. Des Rosiers MH, Kennedy C, Shinohara M, Sokoloff L (1976) Effects of CO2 on local cerebral glucose utilization in the conscious rat. Neurology 26:346

    Google Scholar 

  13. Fabiny DC, Ertingschausen G (1971) Automated reaction-rate method for determination of serum creatinine with the centrifichem. Clin Chem 17:697–700

    Google Scholar 

  14. Fenstermacher JD, Blasberg RG, Patlak CS (1986) Methods for quantifying the transport of drugs across brain barrier systems. In: Goldman ID (ed) Membrane transport of antineoplastic agents: the international encyclopedia of pharmacology and therapeuties. Pergamon, Oxford, pp 113–146

    Google Scholar 

  15. Gross PM, Weindl A (1987) Peering through the windows of the brain. J Cereb Blood Flow Metab 7:663–672

    Google Scholar 

  16. Gross PM, Sposito NM, Pettersen SE, Fenstermacher JD (1986) Differences in function and structure of the pituitary endothelium in the supraoptic nucleus and pituitary neural lobe of rats. Neuroendocrinology 44:401–407

    Google Scholar 

  17. Harbeck RJ, Hoffman AA, Hoffman SA, Shucard DW (1979) Cerebrospinal fluid and the choroid plexus during acute immune complex disease. Clin Immunol Immunopathol 13:413–425

    Google Scholar 

  18. Hoffman SA, Arbogast DN, Day TT, Shucard DW, Harbeck RJ (1983) Permeability of the blood cerebrospinal fluid barrier during acute immune complex disease. J Immunol 130:1695–1698

    Google Scholar 

  19. Hoover RE (1956) Nongranulomatous uveitis; complication of serum sickness. Am J Ophthalmol 41:534–536

    Google Scholar 

  20. Huang JT, Mannik M, Gleisner J (1984) In situ formation of immune complexes in the choroid plexus of rats by sequential injection of a cationized antigen and unaltered antibodies. J Neuropathol Exp Neurol 43:489–499

    Google Scholar 

  21. John KJ, Ward PA (1982) Biology of Disease. Newer concepts in the pathogenesis of immune complex-induced tissue injury. Lab Invest 47:218–226

    Google Scholar 

  22. Kabat EA, Mayer MM (1961) Immunological and immunochemical methodology in experimental immunochemistry, 2nd edn. Thomas, Springfield, p 68

    Google Scholar 

  23. Kanski JJ (1977) Anterior uveitis in juvenile rheumatoid arthritis. Arch Ophthalmol 95:1794–1799

    Google Scholar 

  24. Koff MN, Chernack WJ, Griswold WR, McIntosh RM (1973) The choroid plexus in acute serum sickness. Arch Pathol 96:331–334

    Google Scholar 

  25. Lampert PW, Oldstone MBA (1973) Host immunoglobulin G and complement deposits in the choroid plexus during spontaneous immune complex disease. Science 180:408–410

    Google Scholar 

  26. Ohno K, Pettigrew KD, Rapoport SI (1978) Lower limits of cerebrovascular permeability to nonelectrolytes in the conscious rat. Am J Physiol 235:H299-H307

    Google Scholar 

  27. Peress NS (1980) Immune complex deposition in the ciliary process of rabbits with acute and chronic serum sickness. Exp Eye Res 30:371–378

    Google Scholar 

  28. Peress NS, Tompkins DC (1979) Integrity of the zonulae occludentes of the choroid plexus epithelium and brain endothelium in experimental chronic serum sickness. Neuropathol Appl Neurobiol 5:279–288

    Google Scholar 

  29. Peress NS, Miller F, Palu W (1976) The immunopathophysiological effects of chronic serum sickness on rat choroid plexus, ciliary process and renal glomeruli. J Neuropathol Exp Neurol 36:726–733

    Google Scholar 

  30. Peress NS, Miller F, Palu W (1977) The choroid plexus in passive serum sickness. J Neuropathol Exp Neurol 36:561–566

    Google Scholar 

  31. Peress NS, Perillo E, Fenstermacher JD (1989) Circumventricular organs in chronic serum sickness: a model for cerebral lupus. Biol Psychiatry 26:397–407

    Google Scholar 

  32. Pertschuk LP, Szabo K, Vuletin JC, Rainford EA (1977) Ocular immunopathology in murine lupus. Arch Pathol Lab Med 101:122–124

    Google Scholar 

  33. Savaki HE, Kadekaro M, Jehle J, Sokoloff L (1978) Alpha- and beta-adrenoreceptor blockers have opposite effects on energy metabolism of the central auditory system. Nature 276:521–523

    Google Scholar 

  34. Shinohara M, Dollinger B, Brown G, Rapoport S, Sokoloff L (1979) Cerebral glucose utilization: local changes during and after a recovery from spreading cortical depression. Science 203:188–190

    Google Scholar 

  35. Sokoloff L, Reivich M, Kennedy C, Des Rosiers MH, Patlak CS, Pettigrew KD, Sakurada O, Shinohara M (1977) The [14C]-deoxyglucose method for the measurement of local cerebral glucose utilization: theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28:897–916

    Google Scholar 

  36. Waldrep CJ, Kaplan HJ, Warbington M (1987) In situ immune complex formation within the uvea. Invest Ophthalmol Vis Sci 28:1191–1195

    Google Scholar 

  37. Wong VG, Anderson RR, McMaster PRB (1971) Endongenous immune uveitis: the role of serum sickness. Arch Ophthalmol 85:93–102

    Google Scholar 

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Authors and Affiliations

  1. Department of Neurological Surgery, HSC, State University of New York, T12-080, 11794, Stony Brook, NY, USA

    H. Nakata, A. Shimizu, A. Tajima, S. Z. Lin, K. Gruber & J. Fenstermacher

  2. Department of Pathology, State University of New York, 11794, Stony Brook, NY, USA

    E. Perillo & N. Peress

  3. Veterans Administration Medical Center, 11768, Northport, NY, USA

    E. Perillo & N. Peress

Authors
  1. H. Nakata
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  2. A. Shimizu
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  3. A. Tajima
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  4. S. Z. Lin
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  5. K. Gruber
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  6. E. Perillo
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  7. N. Peress
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  8. J. Fenstermacher
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Supported by NIH Grant NS 21157 and VA Merit Funding

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Nakata, H., Shimizu, A., Tajima, A. et al. The effects of chronic serum sickness on albumin distribution and glucose utilization in rat brain. Acta Neuropathol 81, 312–317 (1991). https://doi.org/10.1007/BF00305873

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  • DOI: https://doi.org/10.1007/BF00305873

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