Skip to main content
SpringerLink
Log in

Immunochemistry of ethylnitrosourea-induced rat neurinomas, the RN6 neurinoma cell line and their transplantation tumors

  • Regular Papers
  • Published:
Acta Neuropathologica Aims and scope Submit manuscript

Summary

The expression of glial fibrillary acidic protein (GFAP), vimentin, S-100 protein (S-100), HNK-1, myelin basic protein (MBP) and fibronectin was investigated immunohistochemically in 51 ethylnitrosourea (ENU)-induced neurinomas of the rat. Additionally, 90 transplantation tumors derived from ENU-induced neurinomas and the RN6 rat neurinoma cell clone were studied. Vimentin immunoreactivity was shown in 50/51 primary neurinomas and 60/90 transplantation tumors. In contrast, GFAP was expressed in only 23/51 primary tumors and in 5/90 transplantation tumors. In the RN6 neurinoma clone, vimentin and GFAP could be demonstrated both in vivo and in vitro GFAP expression varied depending on the tumor localization, i.e., tumors of distal portions of peripheral nerves were more frequently GFAP positive than tumors of the spinal roots or of cranial nerves. The same tendency was observed for S-100. In the series of transplantation tumors S-100 and GFAP immunoreactivity decreased with increasing numbers of transplantation passages. Only individual cells in 5 primary tumors were HNK-1 positive and no MBP-immunorcactive cells were observed. Our results demonstrate that the expression of differentiation antigens in ENU-induced experimental neurinomas parallels the results reported for human neurinomas.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Altmannsberger M, Osborn M, Schauer A, Weber K (1981) Antibodies to different intermediate filament proteins. Lab Invest 45:427–434

    Google Scholar 

  2. Braun M, Grasso A, Wechsler W (1976) 14-3-2 protein in rat primary and transplanted gliomas and neurinomas and in clonal cell lines. Exp Brain Res 25:93–97

    Google Scholar 

  3. Cardesa A, Ribalta T, von Schilling B, Palacin A, Mohr U (1989) Experimental model of tumors associated with neurofibromatosis. Cancer 63:1737–1749

    Google Scholar 

  4. Clark HB, Minesky JJ, Agrawal D, Agrawal HC (1985) Myelin basic protein and P2 protein are not immunohistochemical markers for Schwann cell neoplasms. A comparative study using antisera to S-100, P2, and myelinbasic proteins. Am J Pathol 121:96–121

    Google Scholar 

  5. Conley FK (1979) The immunocytochemical localization of GFA protein in experimental murine CNS tumors. Acta Neuropathol (Berl) 45:9–16

    Google Scholar 

  6. Cravioto H, Weiss JF, Weiss EC, Goebel HH, Ransohoff J (1973) Biological characteristics of peripheral nerve tumors induced with ethylnitrosourea. Acta Neuropathol (Berl) 23:265–280

    Google Scholar 

  7. Daimaru Y, Hashimoto H, Enjoji M (1985) Malignant peripheral nerve-sheath tumors (malignant schwannomas). An immunohistochemical study of 29 cases. Am J Surg Pathol 9:434–444

    Google Scholar 

  8. Daneels G, Moeremans M, deRaeymaeker M, de Mey J (1986) Sequential immunostaining (gold/silver) and complete protein staining (AuroDye) on Western blots. J Immunol Methods 89:89–91

    Google Scholar 

  9. Denlinger RH, Koestner A, Swenberg JA (1973) An experimental model for selective production of neoplasms of the peripheral nervous system. Acta Neuropathol (Berl) 23:219–228

    Google Scholar 

  10. Gabbiani G, Kapanci Y, Baazzone P, Franke WW (1982) Immunochemical identification of intermediate-sized filaments in human neoplastic cells. Am J Pathol 104:206–216

    Google Scholar 

  11. Gould VE, Moll R, Moll I, Lee I, Schwechheimer K, Franke WW (1986) The intermediate filament complement of the spectrum of nerve sheath neoplasms. Lab Invest 55:463–474

    Google Scholar 

  12. Hsu SM, Raine L, Fanger H (1981) The use of anti-avidin antibody and avidin-biotin-peroxidase-complex in immunoperoxidase techniques. Am J Clin Pathol 75:816–821

    Google Scholar 

  13. Jänisch W, Schreiber D (1977) Experimental tumors of the central nervous system. Ed by Bigner DD, Swenberg JA. Upjohn Company, Kalamazoo, MI, p 24

    Google Scholar 

  14. Johnson MD, Glick AD, Davis BW (1988) Immunohistochemical evaluation of Leu-7, myelin basic protein, S-100 protein, glial fibrillary acidic protein and LN3 immunoreactivity in nerve sheath tumors and sarcomas. Arch Lab Pathol Med 112:150–160

    Google Scholar 

  15. Kawahara E, Oda Y, Ooi A, Katsuda S, Nakanishi I, Umeda S (1988) Expression of glial fibrillary acidic protein (GFAP) in peripheral nerve sheath tumors. Am J Surg Pathol 12:115–120

    Google Scholar 

  16. Kyhle-Anderson J (1985) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Methods 10:203–209

    Google Scholar 

  17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  18. Matsunou H, Shimoda T, Kakimoto S, Yamashita H, Ishikawa E, Mukai M (1985) Histopathologic and immunohistochemical study of malignant tumors of peripheral nerve sheath (malignant schwannoma). Cancer 56:2269–2279

    Google Scholar 

  19. Miettinen M, Lehto VP, Virtanen I (1984) Antibodies to intermediate filament proteins in the diagnosis and classification of human tumors. Ultrastruct Pathol 7:83–107

    Google Scholar 

  20. Osborn M, Weber K (1983) Biology of disease. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394

    Google Scholar 

  21. Perentes E, Rubinstein LJ (1985) Immunohistochemical recognition of human nerve sheath tumors by anti-Leu-7 (HNK-1) monoclonal antibody. Acta Neuropathol (Berl) 68:319–324

    Google Scholar 

  22. Perentes E, Rubinstein LJ (1987) Recent applications of immunoperoxidase histochemistry in human neuro-oncology. An update. Arch Pathol Lab Med 111:796–812

    Google Scholar 

  23. Pfeiffer SE, Wechsler W (1972) Isolation of clones from a chemically induced tumor of the peripheral nervous system of the rat. Naturwissenschaften 59:518–519

    Google Scholar 

  24. Pfeiffer SE, Wechsler W (1972) Biochemically differentiated neoplastic clone of Schwann cells. Proc Natl Acad Sci USA 69:2885–2889

    Google Scholar 

  25. Ramaekers FCS, Puts JJG, Kant A, Moesker O, Jap PHK, Vooijs GP (1982) Use of antibodies to intermediate filaments in the characterization of human tumors. Cold Spring Harb Symp Quant Biol 46:331–339

    Google Scholar 

  26. Reifenberger G (1991) Immunhistochemie der Tumoren des Nervensystems. Springer, Berlin, Heidelberg New York Tokyo, pp 2–5

    Google Scholar 

  27. Reifenberger G, Szymas J, Wechsler W (1987) Differential expression of glial-and neuronal-associated antigens in human tumors of the central and peripheral nervous system. Acta Neuropathol (Berl) 74:105–123

    Google Scholar 

  28. Reifenberger G, Bilzer T, Seitz RJ, Wechsler W (1989) Expression of vimentin and glial fibrillary acidic protein in ethylnitrosourea-induced rat gliomas and glioma cell lines. Acata Neuropathol 78:270–282

    Google Scholar 

  29. Schiffer D, Giordana MT, Mauro A, Migheli A, Germano I, Giaccone G (1986) Immunohistochemical demonstration of vimentin in human cerebral tumors. Acta Neuropathol (Berl) 70:209–219

    Google Scholar 

  30. Stanton C, Perentas E, Collin VP, Rubinstein LJ (1987) GFA protein in nerve sheath tumors: a polyvalent and monoclonal antibody study. J Neuropathol Exp Neurol 46:634–643

    Google Scholar 

  31. Stavrou D, Haglid KG, Weidenbach W (1971) The brain specific proteins S-100 and 14.3.2. in experimental brain tumors of the rat. Z Ges Exp Med 156:237–242

    Google Scholar 

  32. Stavrou D, Haglid KG, Rönnbäck L (1973) The S-100 protein in rat brain and in methylnitrosourea-induced tumors of the rat nervous system. Eur Neurol 10:168–178

    Google Scholar 

  33. Sternberger LA (1986) Immunocytochemistry, 3rd ed. Wiley and Sons, New York Chichester Brisbane Toronto, pp 103–114

    Google Scholar 

  34. Swenberg JA, Koestner A, Wechsler W (1972) The induction of tumors of the nervous system with intravenous methylnitrosourea. Lab Invest 26:74–85

    Google Scholar 

  35. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  36. Turusov US (1988) On histogenesis of neurinoma induced by nitrosoethylurea in rats. Vopr Onkol 34:699–705

    Google Scholar 

  37. Wechsler W (1972) S-100 protein in experimental rat tumors of the central and peripheral nervous system in inbred rats. Naturwissenschaften 59:370–371

    Google Scholar 

  38. Wechsler W, Ramadan MA (1971) Ethylnitrosourea-induced transplantable tumors of the peripheral nervous system in inbred rats. Naturwissenschaften 58:547–578

    Google Scholar 

  39. Wechsler W, Kleihues P, Matsumoto S, Zülch KJ, Ivankovic S, Preussmann R, Druckrey H (1969) Pathology of experimental neurogenic tumors chemically induced during prenatal and postnatal life. Ann NY Acad Sci 159:360–408

    Google Scholar 

  40. Wechsler W, Ramadan MA, Gieseler A (1972) Isogenic transplantation of ethylnitrosourea-induced tumors of the central and peripheral nervous system in two different inbred rat strains. Naturwissenschaften 59:474–476

    Google Scholar 

  41. Wechsler W, Pfeiffer SE, Swenberg JA, Koestner A (1973) S-100 protein in methyl-and ethylnitrosourea induced tumors of the rat nervous system. Acta Neuropathol (Berl) 24:287–303

    Google Scholar 

  42. Weiss SW, Langloss JM, Enzinger FM (1983) Value of S-100 protein in the diagnosis of soft tissue tumors with particular reference to benign and malignant schwann cell tumors. Lab Invest 49:299–308

    Google Scholar 

  43. Wick MR, Swanson PE, Scheithauer BW, Manivel JC (1987) Malignant peripheral nerve sheath tumor. An immunohistochemical study of 62 cases. Am J Clin Pathol 87:425–433

    Google Scholar 

  44. Yung WKA, Luna M, Borit A (1985) Vimentin and glial fibrillary acidic protein in human brain tumors. J Neurooncol 3:35–38

    Google Scholar 

  45. Zülch KJ (1979) Histological typing of tumours of the central nervous system. International histological classification of tumours. WHO, Geneva

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Abteilung für Neuropathologie, Heinrich-Heine-Universität, Moorenstraße 5, W-4000, Düsseldorf, Federal Republic of Germany

    K. T. Vogeley, T. Bilzer, G. Reifenberger & W. Wechsler

Authors
  1. K. T. Vogeley
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Bilzer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Reifenberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. Wechsler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vogeley, K.T., Bilzer, T., Reifenberger, G. et al. Immunochemistry of ethylnitrosourea-induced rat neurinomas, the RN6 neurinoma cell line and their transplantation tumors. Acta Neuropathol 82, 78–85 (1991). https://doi.org/10.1007/BF00310927

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00310927

Key words

Search

Navigation