Skip to main content
Log in

Experimental allergic myositis in SJL/J mice immunized with rabbit myosin B fraction: immunohistochemical analysis and transfer

  • Regular Papers
  • Published:
Acta Neuropathologica Aims and scope Submit manuscript

Summary

Experimental allergic myositis (EAM) was produced in SJL/J mice by inoculation with the myosin B fraction of the rabbit skeletal muscle, and the pathological changes were quantified. The myosin B fraction contains actin, myosin, tropomyosin and many other proteins, and has been known to induce severe EAM in guinea pigs. In the present model, macrophages and CD4+ lymphocytes predominated among the infiltrating cells. On the surface of muscle fibers and in the regions of cell infiltration deposition of the immunoglobulin G (IgG) and complement factor 3 was observed. EAM was transferred to normal mice by injecting the serum IgG of EAM. Depleting the recipients of complement before the transfer resulted in less-severe pathological changes. Morphologically, the EAM IgG showed an affinity for the nuclei, myofilaments, sarcolemma and blood vessels of mouse skeletal muscle. Biochemically EAM IgG contained antibodies against myosin, actin, troponin, M protein and other muscle proteins. These results indicated that IgG and complement play important roles in this model.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Behlke MA, Chou HS, Huppi K, Loh DY (1986) Murine T-cell receptor mutants with deletions of beta-chain variable region genes. Proc Natl Acad Sci USA 83: 767–771

    Google Scholar 

  2. Currie S (1971) Experimental myositis. The in vivo and in vitro activity of lymph-node cells. J Pathol 105: 169–185

    Google Scholar 

  3. Dalakas MC (1991) Polymyositis, dermatomyositis, and inclusion-body myositis. N Engl J Med 325: 1487–1498

    Google Scholar 

  4. Dawkins RL (1965) Experimental myositis associated with hypersensitivity to muscle. J Pathol Bacteriol 90: 619–625

    Google Scholar 

  5. Dawkins RL, Loewi G (1973) Cytotoxic effect of antisera on 51Cr-labelled monolayers of skeletal muscle. J Pathol 110: 67–74

    Google Scholar 

  6. de Berardinis P, Londei M, James RF, Lake SP, Wise PH, Feldmann M (1988) Do CD4-positive cytotoxic T cells damage islet beta cells in type 1 diabetes? Lancet II: 823–824

    Google Scholar 

  7. De Mey JR (1983) The preparation of immunoglobulin gold conjugates (IGS reagents) and their use as markers for light and electron microscopic immunocytochemistry. In: Cuello AC (ed) Immunohistochemistry. John Wiley & Sons, Chischester, pp 347–372

    Google Scholar 

  8. Emslie-Smith AM, Engel AG (1990) Microvascular changes in early and advanced dermatomyositis. A quantitative study. Ann Neurol 27: 343–356

    Google Scholar 

  9. Engel AG, Arahata K (1984) Monoclonal antibody analysis of mononuclear cells in myopathies. II. Phenotypes of autoinvasive cells in polymyositis and inclusion body myositis. Ann Neurol 16: 209–215

    Google Scholar 

  10. Esiri MM, MacLennan LCM (1973) Experimental myositis in rats. I. Histological and creatine phosphokinase changes, and passive transfer to normal syngeneic rats. Clin Exp Immunol 17: 139–150

    Google Scholar 

  11. Fahey JL, McKelvey EM (1965) Quantitative determination of serum immunoglobulins in antibody-agar plates. J Immunol 94: 84–90

    Google Scholar 

  12. Haran-Ghera N, Ben-Yaakov M, Peled A, Bentwitch Z (1973) Immune status of SJL/J mice in relation to age and spontaneous tumor development. J Natl Cancer Inst 50: 1227–1235

    Google Scholar 

  13. Hart MN, Linthicum DS, Waldschmidt MM, Tassell SK, Schelper RL, Robinson RA (1987) Experimental autoimmune inflammatory myopathy. J Neuropathol Exp Neurol 46: 511–521

    Google Scholar 

  14. Holfeld R, Mueller W, Toyka KV (1988) Necrotizing myopathy in SJL mice (letter). Muscle Nerve 11: 184–185

    Google Scholar 

  15. Manghani D, Partridge TA, Sloper JC (1974) The role of the myofibrillar fraction of skeletal muscle in the production of experimental polymyositis. J Neurol Sci 23: 489–503

    Google Scholar 

  16. Matsubara S, Takamori M (1987) Experimental allergic myositis: ultrastructural, histochemical, immunological and immunohistochemical studies. Acta Neuropathol (Berl) 74: 151–157

    Google Scholar 

  17. Matsubara S, Takamori M (1987) Experimental allergic myositis: strain 13 guinea pig immunised with rabbit myosin B fraction. Acta Neuropathol (Berl) 74: 158–162

    Google Scholar 

  18. Matsubara S, Takamori M, Adachi H, Kida H (1990) Acute toxoplasma myositis: an immunohistochemical and ultrastructural study. Acta Neuropathol 81: 223–227

    Google Scholar 

  19. McDevitt HO, Peters JH, Pollard LW, Harter JG, Coons AH (1963) Purification and analysis of fluorescein-labeled antisera by column chromatography. J Immunol 90: 634–642

    Google Scholar 

  20. McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immuno-electron microscopy. J Histochem Cytochem 22: 1077–1083

    CAS  PubMed  Google Scholar 

  21. Morgan G, Peter JB, Newbould BB (1971) Experimental allergic myositis in rats. Arthritis Rheum 14: 599–609

    Google Scholar 

  22. Nishikai M, Reichlin M (1980) Heterogeneity of precipitating antibodies in polymyositis and dermatomyositis. Characterization of the Jo-1 antibody system. Arthritis Rheum 23: 881–888

    Google Scholar 

  23. Partridge TA, Smith PD (1976) A quantitative test to detect lymphocytes sensitized against the surface of muscle cells. Clin Exp Immunol 25: 139–143

    Google Scholar 

  24. Rosenberg NL, Kotzin BL (1989) Aberrant expression of class II MHC antigens by skeletal muscle endothelial cells in experimental autoimmune myositis. J Immunol 142: 4289–4294

    Google Scholar 

  25. Rosenberg NL, Ringel SP, Kotzin BL (1987) Experimental autoimmune myositis in SJL/mice. Clin Exp Immunol 68: 117–129

    Google Scholar 

  26. Singer PA, McEvilly RJ, Balderas RS, Dixon FJ, Theofilopoulos AN (1988) T-cell receptor alpha-chain variable-region haplotypes of normal and autoimmune laboratory mouse strains. Proc Natl Acad Sci USA 85: 7729–7733

    Google Scholar 

  27. Sinha AA, Lopez MT, McDevitt HO (1990) Autoimmune diseases. The failure of self tolerance. Science 248: 1380–1388

    Google Scholar 

  28. Smith PD, Partridge TA (1976) Macrophage migration inhibition studies of lymphocytes taken from guinea-pigs suffering from experimental polymyositis. Clin Exp Immunol 25: 133–138

    Google Scholar 

  29. Takayanagi T (1967) Immunohistological studies of experimental myositis in relation to human polymyositis with special reference to passive transfer and fluorescent antibody technique. Folia Psychiatr Neurol Jpn 21: 117–127

    Google Scholar 

  30. Warnke R, Levy R (1980) Detection of T and B cell antigens with hybridoma monoclonal antibodies. A biotin-avidinhorseradisch peroxidase method. J Histochem Cytochem 28: 771–776

    Google Scholar 

  31. Whitaker JN (1982) Inflammatory myopathy. A review of etiologic and pathogenetic factors. Muscle Nerve 5: 573–592

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Supported in part by grant from the Neuroimmunological Disease Research Committee (Japanese Ministry of Health and Welfare). This paper was read at the 11th World Congress of Neuropathology (Kyoto, 1990).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsubara, S., Shima, T. & Takamori, M. Experimental allergic myositis in SJL/J mice immunized with rabbit myosin B fraction: immunohistochemical analysis and transfer. Acta Neuropathol 85, 138–144 (1993). https://doi.org/10.1007/BF00227760

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00227760

Key words

Navigation