Abstract
To determine how and when the satellite cells are stimulated to replicate in muscle regeneration, the rat soleus muscle was examined chronologically after bupivacaine-induced myonecrosis. Bromodeoxyuridine and desmin-positive mononuclear cells, indicating the start of satellite cell replication, were seen 25 h after bupivacaine treatment when macrophages had already invaded the sarcoplasm of necrotic fiber. These findings suggest that muscle regeneration starts as early as the time at which macrophages begin to scavenge necrotic material. Proliferating myoblasts increased in number, reaching a maximum at 49 h after myonecrosis, and decreased in number 3 days after the myoblasts fused with each other to form myotubes. The satellite cell proliferation after bupivacaine-induced myonecrosis began at almost the same time as in crush injury, and earlier than after muscle transplantation using whole intact or minced muscle fragments. The earlier begining and more rapid regenerating process probably resulted from the preservation of intact satellite cells, blood vessels and peripheral nerves in the bupivacaine-induced myonecrosis.
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Akiyama C, Kobayashi S, Nonaka I (1992) Comparison of behavior in muscle fiber regeneration after bupivacaine hydrochloride and acid anhydride-induced myonecrosis. Acta Neuropathol 83:584–589
Allen RE, Rankin LL (1990) Regulation of satellite cells during skeletal muscle growth and development. Proc Soc Exp Biol Med 194:81–86
Anderson JE, Liu L, Kardami E (1991) Distinctive patterns of basic fibroblast growth factor (bFGF) distribution in degenerating and regenerating areas of dystrophic (mdx) striated muscles. Dev Biol 147:96–109
Austin L, Burgess AW (1991) Stimulation of myoblast proliferation in culture by leukaemia inhibitory factor and other cytokines. J Neurol Sci 101:193–197
Benoit PW, Belt WD (1970) Destruction and regeneration of skeletal muscle after treatment with a local anesthetic, bupivacaine (Marcaine). J Anat 107:547–556
Bischoff R (1986) Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol 115:129–139
Bischoff R (1986) A satellite cell mitogen from crushed adult muscle. Dev Biol 115:140–147
Bischoff R (1990) Cell cycle commitment of rat muscle satellite cells. J Cell Biol 111:201–207
Bradley WG (1979) Muscle fiber splitting. In: Mauro A (ed) Muscle regeneration. Raven Press, New York, pp 215–232
Carlson BM (1973) The regeneration of skeletal muscle: a review. Am J Anat 137:119–150
deFazio A, Leary JA, Hedley DW, Tattersall MHN (1987) Immunohistochemical detection of proliferating cells in vivo. J Histochem Cytochem 35:571–577
Foser RF, Thompson JM, Kaufman SJ (1987) A laminin substrate promotes myogenesis in rat skeletal muscle cultures: analysis of replication and development using antidesmin and anti-BrdUrd monoclonal antibodies. Dev Biol 122:11–20
Grounds MD (1987) Phagocytosis of necrotic muscle in muscle isografts is influenced by the strain, age and sex of host mice. J Pathol 153:71–82
Grounds MD (1991) Towards understanding skeletal muscle regeneration. Pathol Res Pract 187:1–22
Grounds MD, McGeachie JK (1987) A model of myogenesis in vivo, derived from detailed autoradiographic studies of regenerating skeletal muscle, challenges the concept of quantal mitosis. Cell Tissue Res 250:563–569
Grounds MD, McGeachie JK (1987) Reutilisation of tritiated thymidine in studies of regenerating skeletal muscle. Cell Tissue Res 250:141–148
Grounds MD, McGeachie JK (1989) A comparison of muscle precursor replication in crush-injured skeletal muscle of Swiss and BALB/c mice. Cell Tissue Res 255:385–391
Grounds MD, McGeachie JK (1990) Myogenic cell replication in minced skeletal muscle isografts of Swiss and BALBc mice. Muscle Nerve 13:305–313
Hall-Craggs ECB (1974) Rapid degeneration and regeneration of a whole skeletal muscle following treatment with bupivacaine (Marcain). Exp Neurol 43:349–358
Hall-Craggs ECB, Seyan HS (1975) Histochemical changes in innervated and denervated skeletal muscle fibers following treatment with bupivacaine (Marcain). Exp Neurol 46:345–354
Helliwell TR (1988) Lectin binding and desmin staining during bupivacaine-induced necrosis and regeneration in rat skeletal muscle. J Pathol 155:317–326
Ishiura S, Nonaka I, Fujita T, Sugita H (1983) Effect of cycloheximide administration on bupivacaine induced acute muscle degradation. J Biochem 94:1631–1636
Jennische E, Skottner A, Hansson H-A (1987) Satellite cells express the trophic factor IGF-I in regenerating skeletal muscle. Acta Physiol Scand 129:9–15
Jirmanova I, Thesleff S (1972) Ultrastructural study of experimental muscle degeneration and regeneration in the adult rat. Z Zellforsch 131:77–97
Jones GH (1982) Protein synthesis in bupivacaine (Marcaine)-treated, regenerating skeletal muscle. Muscle Nerve 5:281–290
Kaufman SJ, Foster RF (1988) Replicating myoblasts express a muscle-specific phenotype. Proc Natl Acad Sci USA 85:9606–9610
McGeachie JK, Grounds MD (1987) Initiation and duration of muscle precursor replication after mild and severe injury to skeletal muscle of mice: an autoradiographic study. Cell Tissue Res 248:125–130
Mitchell CA, McGeachie JK, Grounds MD (1992) Cellular differences in the regeneration of murine skeletal muscle: a quantitative histological study in SJL/J and BALB/c mice. Cell Tissue Res 269:159–166
Nathan CF (1987) Secretory products of macrophages. J Clin Invest 79:319–326
Nonaka I, Takagi A, Ishiura S, Nakase H, Sugita H (1983) Pathophysiology of muscle fiber necrosis induced by bupivacaine hydrochloride (Marcaine). Acta Neuropathol (Berl) 60:167–174
Orimo S, Hiyamuta E, Arahata K, Sugita H (1991) Analysis of inflammatory cells and complement C3 in bupivacaine-induced myonecrosis. Muscle Nerve 14:515–520
Schultz E, Jaryszak DL, Valliere CR (1985) Response of satellite cells to focal skeletal muscle injury. Muscle Nerve 8:217–222
Shultz E, Jaryszak DL, Gibson MC, Albright DJ (1986) Absence of exogenous satellite cell contribution to regeneration of frozen skeletal muscle. J Muscle Res Cell Motil 7:361–367
Yablonka-Reuveni Z, Nameroff M (1986) Immunocytochemical studies on the expression of desmin by dividing cells from skeletal muscle. In: Emerson C, Fishman D, Nadal-Ginard B, Siddiqui MAQ (eds) Molecular biology of muscle development. Liss, New York, pp 47–60
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Saito, Y., Nonaka, I. Initiation of satellite cell replication in bupivacaine-induced myonecrosis. Acta Neuropathol 88, 252–257 (1994). https://doi.org/10.1007/BF00293401
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DOI: https://doi.org/10.1007/BF00293401