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Alzheimer paired helical filaments: a comparison with the twisted ribbon model

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Abstract

To investigate if Alzheimer paired helical filaments (PHF) closely resemble twisted ribbons, as indicated by recent high-resolution ultrastructural studies, we compared physical models of twisted ribbons with electron microscopic images of PHF. Uranyl-acetate-stained, isolated PHF with one or two helical turns were compared with scale models of twisted ribbons with one and two helical turns rotated at different angles. The various rotations of the twisted ribbon model corresponded well with the different orientations of randomly dispersed PHF. The electron-dense regions of individual PHF turns previously thought to represent a cross-over site of paired filaments corresponded to the edge of the twisted ribbon when the ribbon was oriented perpendicular to the filament axis. These data indicate that the overall configuration of PHF is a twisted ribbon but does not exclude possible configuration restrictions due to an ordered arrangement of subunits.

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Authors and Affiliations

  1. Center for Research in Neurodegenerative Diseases, and the Department of Pathology, Tanz Neuroscience Building, Room 121, University of Toronto, 6 Queen's Park Cres. West, M5S 1A8, Toronto, Ontario, Canada

    M. S. Pollanen & C. Bergeron

  2. Department of Chemistry, University of Toronto, Toronto, Ontario, Canada

    P. Markiewicz & M. C. Goh

Authors
  1. M. S. Pollanen
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  2. P. Markiewicz
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  3. M. C. Goh
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  4. C. Bergeron
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Pollanen, M.S., Markiewicz, P., Goh, M.C. et al. Alzheimer paired helical filaments: a comparison with the twisted ribbon model. Acta Neuropathol 90, 194–197 (1995). https://doi.org/10.1007/BF00294320

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  • DOI: https://doi.org/10.1007/BF00294320

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