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Human epidermal blister: A convenient tissue for toxicological and genetic studies of benzo(a)pyrene metabolism

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Summary

Benzo(a)pyrene (BP) metabolism has been studied in epidermal blisters maintained in a culture medium for 24 h and 48 h. The viability of the cells has been assayed by [3H]proline incorporation into proteins and by [14C]BP metabolism into unconjugated metabolites. A screen of BP metabolism in 19 individuals shows a great variation of basal epidermal activity. Induction of BP metabolism by the application of coal tar 24 h before the epidermal blister sampling, resulted in two- to eight-fold increase in BP metabolism. This induction is not increased when the coal tar application is repeated.

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Authors and Affiliations

  1. Laboratoire de Chimie Médicale, Institut de Pathologie, Université de Liège, B 23, B-4000, Sart Tilman par Liège 1, Belgium

    J. Van Cantfort & J. E. Gielen

  2. Service de Dermatologie, Hôpital de Bavière, B-4020, Liege, Belgium

    T. Lorand & C. M. Lapière

Authors
  1. J. Van Cantfort
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  2. T. Lorand
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  3. J. E. Gielen
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  4. C. M. Lapière
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Van Cantfort, J., Lorand, T., Gielen, J.E. et al. Human epidermal blister: A convenient tissue for toxicological and genetic studies of benzo(a)pyrene metabolism. Arch Dermatol Res 278, 324–328 (1986). https://doi.org/10.1007/BF00407747

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  • DOI: https://doi.org/10.1007/BF00407747

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