Summary
The effect of some agents, influencing the cyclic adenosine 3′,5′-monophosphate (cAMP) content of human cells, on the ability of the keratinocytes of binding pemphigus antibodies was studied by using tissue cultures of rabbit esophagus. As demonstrated by immunofluorescence (IF) for IgG, the bound antibodies appeared markedly decreased on esophagus explants grown under standard conditions, that is without test agents, when compared to ones fixed on fresh esophagus. But the IF reaction was remarkably more intense when methylxanthines or epinephrine were added to the growth medium of the cultures. Following the addition of these agents to the cultures some histologic modifications appeared in the explants, indicating that the keratinization process had probably been stimulated. This temporal relationship of immunofluorescence and histologic findings seems to suggest the hypothesis that keratinocyte differentiation, regulation of cAMP intracellular content, and pemphigus antibodies fixation are related processes.
Similar content being viewed by others
References
Anderson WB, Russel TR, Carchman RA, Pastan I (1973) Interrelationship between adenylate cyclase activity, adenosine 3′:5′-cyclic monophosphate phosphodiesterase activity, adenosine 3′:5′-cyclic monophosphate levels, and growth of cells in culture. Proc Natl Acad Sci USA 70:3802–3816
Bauer FW, De Grood RM (1973) Alterations of subcultured keratinocytes to a keratinizing epithelium. Br J Dermatol 89:29–32
Clark RB, Su YF, Ortmann R, Cubeddu XL, Johnson GL, Perkins JP (1975) Factors influencing the effect of hormones on the accumulation of cyclic AMP in cultured human astrocytoma cells. Metabolism 24:343–358
Constable H, Cooper JR, Cruickshand CND., Mann P (1974) Keratinization in dispersed cell cultures of adult guinea pig ear skin. Br J Dermatol 91:39–48
Delescluse C, Colburn NH, Duell EA, Voorhees JJ (1974) Cyclic AMP-elevating agents inhibit proliferation of keratinizing guinea pig epidermal cells. Differentiation 2:343–350
Delescluse C, Fukuyama K, Epstein WL (1976) Dibutyryl cyclic AMP-induced differentiation of epidermal cells in tissue culture. J Invest Dermatol 66:8–13
Delescluse G, Pruniéras M (1976) Effet de l'AMP cyclique sur la différenciation des cellules épidermiques en culture. Ann Dermatol Syph 103:601–603
De Moragas JM, Winkelmann RH, Jordon RE (1970) Immunofluorescence of epithelial skin tumours. I. Patterns of intercellular substance. Cancer 25:1399–1403
Deng JS, Beutner EH, Shu S, Chorzelski TP (1977) Pemphigus antibody action on skin explants. Arch Dermatol 113:923–926
Flaxman AB, Harper RA (1975) In vitro analysis of the control of keratinocyte proliferation in human epidermis by physiologic and pharmacologic agents. J Invest Dermatol 65:52–59
Giannotti B (1976) La patogenesi del pemfigo, dei pemfigoidi e del lupus eritematoso alla luce delle acquisizioni immunopatologiche. In: 53° Congr SIDES La patogenesi autoimmune in dermatologia. G Min Dermatol 111:802–838
Hentzer B, Kobayasi T (1979) Adult human skin maintained in organ culture: I. The ultrastructure of the acellular compartment of connective tissue. Acta Derm Venereol 59:389–400
Hentzer B, Kobayasi T (1980) Adult human skin maintained in organ culture: I. The ultrastructure of the cellular compartment of connective tissue. Acta Derm Venereol 60:465–476
Karasek MA, Charlton ME (1971) Growth of postembryonic skin epithelial cells on collagen gels. J Invest Dermatol 56:205–210
Kelly LA, Butcher RW (1974) The effects of epinephrine and prostaglandin E1 on cyclic adenosine 3′:5′-monophosphate levels in WI-38 fibroblasts. J Biol Chem 249:3098–3102
Kelly LA, Butcher RW (1975) Studies on cyclic AMP metabolism in human epidermoid carcinoma (Hep-2) cells. Metabolism 24:359–368
Kitano Y (1976) Effects of dibutyryl adenosine 3′,5′-cyclic monophosphate on human melanocytes in vitro. Acta Derm Venereol 53:223–228
Makman MH (1971) Conditions leading to enhanced response to glucagon, epinephrine, or protaglandins by adenylate cyclase of normal and malignant cultured cells. Proc Natl Acad Sci USA 68:2127–2135
Makman MH, Klein MI (1972) Expression of adenylate cyclase, catecholamine receptor, and cyclic adenosine monophosphate-dependent protein kinase in synchronized culture of Chang's liver cells. Proc Natl Acad Sci USA 69:456–469
Muller HK, Sutherland RC (1971) Epidermal antigens in cutaneous dysplasia and neoplasia. Nature 230:384–388
Otten J, Johnson GS, Patstan I (1972) Regulation of cell growth by cyclic adenosine 3′:5′-monophosphate. J Biol Chem 247:7082–7095
Sarkany I, Gaylarde PM (1970) Ultrastructural changes in human skin maintained in organ culture. Br J Dermatol 83:572–581
Sbano E, Andreassi L, Baiocchi R, Fimiani M (1978) Anticorps du pemphigus et cellules épithéliales en culture: effets de la théophylline. Les colloques de l'INSERM: Immunopathologie cutanée. INSERM 80:93–100
Seifert W, Paul D (1972) Levels of cyclic AMP in sparse and dense cultures of growing and quiescent 3T3 cells. Nature (New Biol) 240:281–286
Sheppard JR, Prescott DM (1972) Cyclic AMP levels in synchronized mammalian cells. Exp Cell Res 75:293–297
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sbano, E., Andreassi, L., Fimiani, M. et al. Pemphigus antibodies fixation and keratinocyte differentiation in organ cultures. Arch Dermatol Res 273, 9–14 (1982). https://doi.org/10.1007/BF00509022
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00509022