Summary
Tissue distribution of type V collagen in normal human skin was studied using an indirect immunofluorescent technique to determine whether type V collagen is present in the interstitium or in the basement membrane. Type V collagen was isolated from the human placenta by pepsin digestion and was purified with fractioning salt precipitations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that type V collagen contained α1(V) and α2(V) chains, but not the α3(V) chain. Specificity of the rabbit antibodies to type V collagen was assessed using enzyme-linked immunosorbent assay (ELISA) and an immunoblotting method. Antibodies showed no cross-reactivity to other collagens, laminin, and fibronectin. With an indirect immunofluorescent technique, type V collagen was found to be widely distributed throughout the dermis. Intense fluorescent staining was noted in the papillary dermis and adnexal dermis surrounding hair follicles and eccrine glands. The basement membrane of the dermoepidermal junction, skin appendages, and capillaries was not stained. By indirect immunoperoxidase double staining, type V collagen was not found to be deposited on type IV collagen present in the basement membrane. Immunoelectron microscopic studies showed that type V collagen was not located in the basal lamina. These results suggest that type V collagen is distributed in the interstitium, but not in the basement membrane of normal human skin.
Similar content being viewed by others
References
Bonadio JF, Sage H, Cheng F, Bernstein J, Striker GE (1984) Localization of collagen type IV and V, laminin and heparan sulfate proteoglycan to the basal lamina of kidney epithelial cells in transfilter metanephric culture. Am J Pathol 116:289–296
Brown RA, Weiss JB (1979) Type V collagen: possible shared identity of αA, αB and αC chains. FEBS Lett 106:71–75
Burgeson RE, El Adli FA, Kaitila II, Hollister DW (1976) Fetal membrane collagens: identification of two new collagen alpha chains. Proc Natl Acad Sci USA 73:2579–2583
Chung E, Rhodes RK, Miller EJ (1976) Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem Biophys Res Commun 71:1167–1174
Fruto DK, Miller EJ (1980) Isolation of a unique collagenous fraction from limited pepsin digests of human placental tissue. J Biol Chem 255:290–295
Harper JI, Duance VC, Sims TJ, Light ND (1985) Lipoid proteinosis: An inherited disorder of collagen metabolism? Br J Dermatol 113:141–151
Hong BS, Davison PF, Cannon DJ (1979) Isolation and characterization of a distinct type of collagen from bovine fetal membranes and other tissues. Biochemistry 18:4278–4282
Konomi H, Hayashi T, Nakayasu K, Arima M (1984) Localization of type V collagen and type IV collagen in human cornea, lung and skin. Am J Pathol 116:417–426
Linsenmayer TF, Fitch JM, Schmid TM, Zak NB, Gibney E, Sanderson RD, Mayne R (1983) Monoclonal antibodies against chicken type V collagen: production, specificity, and use for immunocytochemical localization in embryonic cornea and other organs. J Cell Biol 96:124–132
Madri JA, Furthmayr H (1979) Isolation and tissue localization of type AB2 collagen from normal lung parenchyma. Am J Pathol 94:323–332
Martinez-Hernandez A, Gay S, Miller EJ (1982) Ultrastructural localization of type V collagen in rat kidney. J Cell Biol 92:343–349
Mayne R, Vail MS, Miller EJ (1978) Characterization of the collagen chains synthesized by cultured smooth muscle cells derived from rhesus monkey thoracic aorta. Biochemistry 17:446–452
Maxwell WL, Duance VC, Lehto M, Ashurst DE, Berry M (1984) The distribution of types I, III, IV and V collagens in penetrant lesions of the central nervous system of the rat. Histochem J 16:1219–1229
Miller EJ, Rhodes RK (1982) Preparation and characterization of the different types of collagen. Methods Enzymol 82:33–64
Modesti A, Kalebic T, Scarpa S, Togo S, Grotendorst G, Liotta LA, Triche TJ (1984) Type V collagen in human amnion is a 12 nm fibrillar component of pericellular intestitium. Eur J Cell Biol 35:246–255
Ooshima A (1981) Collagen αB chain: increased proportion in human atherosclerosis. Science 213:666–668
Peltonen J, Aho H, Halme T, Näntö-Salnen K, Lehto M, Foidart JM, Duance V, Vaheri A, Penttinen R (1984) Distribution of different collagen types and fibronectin in neurofibromatosis tumour. Acta Pathol Microbiol Immunol Scand [Sect A] 92:345–352
Rhodes RK, Miller EJ (1978) Physicochemical characterization and molecular organization of the collagen A and B chains. Biochemistry 17:3442–3448
Roll FJ, Madri JA, Albert J, Furthmayr H (1980) Codistribution of collagen types IV and AB2 in basement membrane and mesangium of the kidney. J Cell Biol 85:597–616
Sage H, Bornstein P (1979) Characterization of a novel collagen chain human placenta and its relation to AB collagen. Biochemistry 18:3815–3822
Sano J, Fujiwara S, Sato S, Ishizaki M, Sugisaki Y, Yajima G, Nagai Y (1981) AB (type V) and basement membrane (type IV) collagens in bovine lung parenchyma: electron microscopic localization by the peroxidase-labeled antibody method. Biomed Res 2:20–29
Schuppan D, Becker J, Boehm H, Hahn EG (1986) Immunofluorescent localization of type-V collagen as a fibrillar component of the interstitial connective tissue of human oral mucosa, artery and liver. Cell Tissue Res 243:535–543
Smith LT, Holbrook KA, Madri JA (1986) Collagen types I, III, and V in human embryonic and fetal skin. Am J Anat 175:507–521
Van Cauwenberge D, Pierard GE, Foidart JM, Lapiere CHM (1983) Immunohistochemical localization of laminin, type IV and type V collagen in basal cell carcinoma. Br J Dermatol 108:163–170
Von der Mark K, Öcalan M (1982) Immunofluorescent localization of type V collagen in the chick embryo with monoclonal antibodies. Collag Rel Res 2:541–555
Wick G, Glanville RW, Timple R (1980) Characterization of antibodies to basement membrane (type IV) collagen in immunological studies. Immunobiology 156:372–381
Woodly DT, Scheidt VJ, Reese MJ, Paller AS, Manning TO, Yoshiike T (1987) Localization of the alpha 3(V) chain of type V collagen in human skin. J Invest Dermatol 88:246–252
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chanoki, M., Ishii, M., Fukai, K. et al. Immunohistochemical localization of type V collagen in normal human skin. Arch Dermatol Res 280, 145–151 (1988). https://doi.org/10.1007/BF00456844
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00456844