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125Iodine decay in DNA: A discussion of its effectiveness for the breaking of DNA strands

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Summary

125I incorporated in DNA is known to be exceptionally toxic. Values of D0 range from about 40 to about 90 decays for survival of mammalian cells. The effectiveness of125I in DNA with respect to the induction of breaks of the DNA strands, however, appears to be comparatively low. The numbers of strand breaks per energy deposited in subnuclear cellular structures such as DNA is smaller for a disintegration of125I than forγ-rays. The difference in effectiveness diminishes with increasing mass of the considered sensitive volume. The apparent inefficiency of125I-decay may, on one hand, result from a waste of local energy deposition. On the other hand, it may be caused by a multitude of local strand breaks (clusters) induced by125I-decay which are measured as one break only by the conventionally applied techniques of strand break measurement. The apparent inefficiency of125I may be evidence furthermore for the importance of considering not only the DNA as the sensitive target but with equal pertinence the gross sensitive volume, i.e. the whole cell nucleus [12]. Further, for drawing meaningful comparisons, it may be necessary to take into consideration the microdosimetric event size distributions for the critical targets [1].

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References

  1. Bond VP, Varma MN, Sondhaus CA, Feinendegen LE (1985) An alternative to absorbed dose, quality, and RBE at low exposures. Radiat Res Suppl 8:104, S52-S57

    Google Scholar 

  2. Bonura T, Town CD, Smith KC, Kaplan HS (1975) The influence of oxygen on the yield of DNA double-strand breaks in X-irradiated Escherichia coli K-12. Radiat Res 63:567–577

    Google Scholar 

  3. Booz J, Paretzke H, Pomplun E, Olko P (1987) Auger-electron cascades, charge potential and microdosimetry of iodine-125. Radiat Environ Biophys 426:151–162

    Google Scholar 

  4. Burki HJ, Roots R, Feinendegen LE, Bond VP (1973) Inactivation of mammalian cells after disintegrations of3H or125I in cell DNA at −196° C. Int J Radiat Biol 24:363–375

    Google Scholar 

  5. Burki HJ, Bunker S, Ritter M, Cleaver JE (1975) DNA damage from incorporated radioisotopes: influence of the3H location in the cell. Radiat Res 62:299–312

    Google Scholar 

  6. Charlton DE, Booz J (1981) A Monte Carlo treatment of the decay of125I. Radiat Res 87:10–23

    Google Scholar 

  7. Charlton DE, Booz J, Fidorra J, Smit Th, Feinendegen LE (1978) Microdosimetry of radioactive nuclei incorporated into the DNA of mammalian cells. In: Booz J, Ebert HG (eds) Sixth Symposium on Microdosimetry, Brussels, Harwood, pp. 91–110

  8. Coquerelle T, Bopp A, Kessler B, Hagen U (1973) Strand breaks and 5′ end-groups in DNA of irradiated thymocytes. Int J Radiat Biol 24:397–404

    Google Scholar 

  9. Deutzmann R, Stöcklin G (1981) Chemical effects of iodine-125 decay in aqueous solution of 5-iodouracil. Ring fragmentation as a consequence of the Auger effect. Radiat Res 87:24–36

    Google Scholar 

  10. Feinendegen LE (1975) Biological damage from the Auger effect, possible benefits. Radiat Environ Biophys 12:85–89

    Google Scholar 

  11. Feinendegen LE, Henneberg P, Tisljar-Lentulis G (1977) DNA strand breakage and repair in human kidney cells after exposure to incorporated iodine-125 and cobalt-60γ-rays. Curr Top Radiat Res 12:436–452

    Google Scholar 

  12. Feinendegen LE, Booz J, Bond VP, Sondhaus CA (1985) Microdosimetric approach to the analysis of responses at low dose and low dose rate. Radiat Prot Dosim 13:299–306

    Google Scholar 

  13. Feinendegen LE, Bond VP, Sondhaus CA (1986) The critical cell concept and its application in the assessment of effects from different dose rates and different radiation qualities. Ann NY Acad Sci 459:211–220

    Google Scholar 

  14. Fidorra J, Mielke Th, Booz J, Feinendegen LE (1981) Cellular and nuclear volume of human cells during the cell cycle. Radiat Environ Biophys 19:205–214

    Google Scholar 

  15. Goodhead DT, Charlton DE, Wilson WE, Paretzke HG (1984) Current biophysical approaches to the understanding of biological effects of radiation in terms of local energy deposition. In: Schraube H, Burger G, Booz J (eds) Fifth Symposium on Neutron Dosimetry (München/Neuherberg); EUR 9762, 1985

  16. Goodhead DT, Charlton DE (1985) Analysis of high-LET radiation effects in terms of local energy deposition. Radiat Prot Dosim 13:253–258

    Google Scholar 

  17. Kornberg RD, King A (1981) The nucleosome. Sci Am 244:48–60

    Google Scholar 

  18. Krisch RE, Krasin F, Sauri CJ (1976) DNA breakage, repair and lethality after125I decay in rec′ and recA strains of Escherichia coli. Int J Radiat Biol 29:37–50

    Google Scholar 

  19. Linz U, Stöcklin G (1985) Chemical and biological consequences of the radioactive decay of iodine-125 in plasmid DNA. Radiat Res 101:262–278

    Google Scholar 

  20. Martin RF, Bradley T, Hodgson GS (1979) Cytotoxicity of an125I-labeled DNA-binding compound that induces double stranded DNA breaks. Cancer Res 39:3244–3247

    Google Scholar 

  21. Martin RF, Haseltine WA (1981) Range of radiochemical damage to DNA with decay of iodine-125. Science 213:896–898

    Google Scholar 

  22. Miyazaki N, Fujiwara Y (1981) Mutagenic and lethal effects of (5-125I)Iodo-2′-deoxyuridine incorporated into DNA of mammalian cells, and their RBEs. Radiat Res 88:456–465

    Google Scholar 

  23. Painter RB, Young BR, Burki HJ (1974) Non-repairable strand breaks induced by125I incorporated into mammalian DNA. Proc Natl Acad Sci USA 71:4836–4838

    Google Scholar 

  24. Pomplun E, Booz J, Charlton DE (1987) A Monte-Carlo simulation of Auger cascades. Radiat Res (accepted for publication)

  25. Ritter MA, Cleaver JE, Tobias CA (1977) High-LET radiations induce a large proportion of non-rejoining DNA breaks. Nature 266:653–655

    Google Scholar 

  26. Roots R, Smith KC (1974) On the nature of the oxygen effect on X-ray-induced DNA single-strand breaks in mammalian cells. Int J Radiat Biol 26:467–480

    Google Scholar 

  27. Roots R, Yang TC, Craise L, Blakely EA, Tobias CA (1979) Impaired repair capacity of DNA breaks induced in mammalian cellular DNA by accelerated heavy ions. Radiat Res 78:38–49

    Google Scholar 

  28. Rydberg B (1980) Detection of induced DNA strand breaks with improved sensitivity in human cells. Radiat Res 81:492–495

    Google Scholar 

  29. Rydberg B (1985) DNA strand breaks induced by low-energy heavy ions. Int J Radiat Biol 47:57–61

    Google Scholar 

  30. Schneeweiss FHA, Myers DK, Tisljar-Lentulis G, Feinendegen LE (1985) Low oxygen enhancement ratios for strand breaks induced by decays of125I in DNA of human T1 cells stored at 0° C. Radiat Prot Dosim 13:237–239

    Google Scholar 

  31. Sundell-Bergmann S, Johanson KJ (1980) Repairable and unrepairable DNA strand breaks induced by decay of3H and125I incorporated into DNA of mammalian cells. Radiat Environ Biophys 18:239–248

    Google Scholar 

  32. Tisljar-Lentulis G, Henneberg P, Mielke Th, Feinendegen LE (1978) DNA strand breaks induced by125I in cultured human kidney cells and their repair. In: Booz J, Ebert HG (eds) Sixth Symposium on Microdosimetry, Brussels. Harwood, pp. 111–120

  33. Tisljar-Lentulis G, Henneberg P, Mielke Th, Feinendegen LE (1980) The oxygen effect in cultured human cells exposed to125I and3H incorporated in DNA. In: Booz J, Ebert HG, Hartfiel HD (eds) Seventh Symposium on Microdosimetry, Oxford. Harwood, pp. 1013–1032

  34. Turner GN, Nobis P, Dewey WC (1976) Fragmentation of chromatin with125I radioactive disintegrations. Biophys J 16:1003–1012

    Google Scholar 

  35. Veatch W, Okada S (1969) Radiation-Induced breaks of DNA in cultured mammalian cells. Biophys J 9:330–346

    Google Scholar 

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Authors and Affiliations

  1. Kernforschungsanlage Jülich GmbH, Institut für Medizin, Postfach 1913, D-5170, Jülich, Germany

    G. Tisljar-Lentulis, F. H. A. Schneeweiss & L. E. Feinendegen

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  1. G. Tisljar-Lentulis
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  2. F. H. A. Schneeweiss
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  3. L. E. Feinendegen
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Dedicated to Prof. L.E. Feinendegen on the occasion of his 60th birthday

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Tisljar-Lentulis, G., Schneeweiss, F.H.A. & Feinendegen, L.E. 125Iodine decay in DNA: A discussion of its effectiveness for the breaking of DNA strands. Radiat Environ Biophys 26, 189–195 (1987). https://doi.org/10.1007/BF01213705

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  • DOI: https://doi.org/10.1007/BF01213705

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