Abstract
Irradiation of embryo cells of Schistocerca gregaria in the two hours before mitotic anaphase produces a characteristic series of responses. An apparently insensitive stage is followed by the production of “stickiness” and side-arm bridges and finally by mitotic delay. Mitotic delay is complete at doses as low as 25 R. It is argued that “stickiness’ is due to a generalised physiological process and that side-arm bridges are due to a localised exchange of discrete sub-units of the chromatid. These conclusions are based upon the kinetics of appearance and dose-response and a comparison of haploid and diploid cells in a haplo-diploid mosaic embryo.
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References
Attwood, K. C.: Numbers of nuclear sites for aberration formation and the distribution of aberrations. In: Radiation induced chromosome aberrations, ed. S. Wolff, p. 73–86. New York: Columbia University Press 1963.
Bajer, A.: Sub-chromatid structure of chromosomes in the living state. Chromosoma (Berl.) 17, 291–302 (1965).
Baker, J. R.: Cytological technique, 3rd ed., p. 182. London: Methuen 1950.
Bergerard, J., et J. Seugé: La parthénogenèse accidentelle chez Locusta migratoria L. Bull. biol. France et Belg. 90, 16–37 (1959).
Boss, J. M. N.: The contribution of the chromosomes to the telophase nucleus in cultures of fibroblasts of the adult crested newt Triturus cristatus carnifax. Exp. Cell Res. 18, 197–216 (1959).
Brock, R. D.: Chromosome balance and endosperm failure in hyacinths. Heredity 9, 199–222 (1955).
Carlson, J. G.: Immediate effects of 250 r of X-rays on the different stages of mitosis in neuroblasts of the grasshopper Chortophaga viridifasciata. J. Morph. 66, 11–23 (1940); - Immediate effects on division morphology and viability of the cell. In: Radiation biology (ed. A. Hollaender), vol. 1 (2), p. 763–824. New York: McGraw-Hill Book Co. 1954.
Conger, A. D.: The fate of metaphase aberrations. Radiat. Bot. 5, 81–96 (1965).
—, and L. M. Fairchild: A quick-freeze method for making smear slides permanent. Stain Technol. 28, 281–283 (1953).
Crouse, H. V.: X-ray breakage of lily chromosomes at first meiotic metaphase. Science 119, 485–487 (1954);- Irradiation of condensed meiotic chromosomes in Lilium longiflorum. Chromosoma (Berl.) 12, 190–214 (1961).
Evans, H. J.: Chromatid aberrations induced by γ-radiation. I. The structure and frequency of chromatid interchanges in diploid and tetraploid cells of Vicia faba. Genetics 46, 257–275 (1961); - Chromosome aberrations induced by ionizing radiations. Int. Rev. Cytol. 13, 221–321 (1962).
—, and J. R. K. Savage: The relation between DNA synthesis and chromosome structure as resolved by X-ray damage. J. Cell Biol. 18, 525–540 (1963).
Giménez-Martín, G., and J. F. López-Sáez: Chromosome structure in the course of mitosis. Cytologia (Tokyo) 30, 14–22 (1965).
Hughes-Schrader, S.: Cytology of coccids (Coccoidea-Homoptera). Advanc. Genet. 2, 127–203 (1948).
John, B., and K. R. Lewis: The meiotic system. Protoplasmatologia 6, F 1 (1965).
LaCour, L. F., and A. Rutishauser: X-ray breakage experiments with endosperm. I. Sub-chromatid breakage. Chromosoma (Berl.) 6, 696–709 (1954).
Lewis, K. R., and B. John: The meiotic consequences of spontaneous chromosome breakage. Chromosoma (Berl.) 18, 287–304 (1966).
Maguire, M. P.: Double-strandedness of meiotic prophase chromatids to light microscope optics and its relationship to genetic recombination. Proc. nat. Acad. Sci. (Wash.) 55, 44–50 (1966).
Manton, I.: New evidence on the telophase split in Todea barbara. Amer. J. Bot. 32, 342–348 (1945).
Melander, Y.: Chromosomal behaviour during the origin of sex chromatin in the rabbit. Hereditas (Lund) 48, 645–661 (1962); - Chromatid tension and fragmentation during the development of Calliphora erythrocephala Meig. (Diptera). Hereditas (Lund) 49, 91–106 (1963a); - Cytogenetic aspects of embryogenesis in Paludicola, Tricladida. Hereditas (Lund) 49, 119–166 (1963b).
Neary, G. J.: The relation between the exponent of dose response for chromosome aberrations and the relative contribution of “two-track” and “one-track” processes. Mutation Res. 2, 242–246 (1965).
Nebel, B. R., and M. L. Ruttle: Chromosome structure. IX. Tradescantia reflexa and Trillium erectum. Amer. J. Bot. 23, 652–663 (1935).
Östergren, G., and T. Wakonig: True or apparent sub-chromatid breakage and the induction of labile states in cytological chromosome loci. Bot. Not. (Lund) 4, 357–375 (1954).
Ohno, S., W. D. Kaplan, and R. Kinosita: Demonstration of bi-partite spiral structure on spermatogonial anaphase chromosomes of Mus musculus. Exp. Cell Res. 15, 426–428 (1958).
Peacock, W. J.: Sub-chromatid structure and chromosome duplication in Vicia faba. Nature (Lond.) 191, 832–833 (1961).
Rao, B., and R. T. Hinegardner: Analysis of DNA synthesis and X-ray induced mitotic delay in sea urchin eggs. Radiat. Res. 26, 534–537 (1965).
Revell, S. H.: A new hypothesis for ‘chromatid’ changes. In: Radiobiology Symposium Liége 1954 (eds. Z. M. Bacq and P. Alexander) p. 243–253. London: Butterworth & Co. 1955; - A new hypothesis for the interpretation of chromatid aberrations, and its relevance to theories for the mode of action of chemical agents. Ann. N.Y. Acad. Sci. 68, 802–810 (1958); - Evidence for a dose-squared term in the dose-response curve for real chromatid discontinuities induced by X-rays, and some theoretical considerations thereof. Mutation Res. 3, 34–53 (1966).
Sax, K., and K. Mather: An X-ray analysis of progressive chromosome splitting. J. Genet. 37, 483–490 (1939).
Sharma, T.: Parthenogenetic haploid embryos in the grasshopper, Aiolopus sp. Sci. and Culture (Calcutta) 28, 345–346 (1962).
Swanson, C. P.: X-ray and ultraviolet studies on pollen tube chromosome. II. The quadripartite structure of prophase chromosomes of Tradescantia. Proc. nat. Acad. Sci. (Wash.) 33, 229–232 (1947).
Trosko, J. E., and S. Wolff: Strandedness of Vicia faba chromosomes as revealed by enzyme digestion studies. J. Cell Biol. 26, 125–135 (1965).
Wolff, S.: Interpretation of induced chromosome breakage and rejoining. Radiat. Res., Suppl. 1, 453–462 (1959); - The kinetics for two break chromosome exchanges, and the 3/2 power rule. In: Repair from genetic radiation damage (ed. F. H. Sobels) p. 1–10. Oxford: Pergamon Press 1963.
—, and H. E. Luippold: Inaccuracy of anaphase bridges as a measure of radiation-induced nuclear damage. Nature (London) 179, 208–209 (1957); - Chromosome splitting as revealed by combined X-ray and labelling experiments. Exp. Cell Res. 34, 548–556 (1964).
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Fox, D.P. The effects of X-rays on the chromosomes of locust embryos. Chromosoma 19, 300–316 (1966). https://doi.org/10.1007/BF00326920
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DOI: https://doi.org/10.1007/BF00326920