Abstract
Two human ring chromosomes 18 were isolated intact in a Langmuir trough, critical-point dried, and studied by quantitative electron microscopy. The portion of deleted genetic material is calculated from dry-mass determinations and is found to be comparable to the total genetic material present in the bacterium Salmonella typhimurium.
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Anderson, T. F.: Techniques for preservation of 3-dimensional structure in preparing specimes for electron microscopy. Trans. N. Y. Acad. Sci., Ser. II 13, 130–134 (1951).
Bahr, G. E.: Human chromosomal fibers. Exp. Cell Res. 62, 39–49 (1970).
—: Golomb, H. M.: Karyotyping of single human chromosomes from dry mass determined by electron microscopy. Proc. nat. Acad. Sci. (Wash.) 68, 726–730 (1971).
—, Zeitler, E.: The determination of the dry mass in populations of isolated particles. Lab. Invest. 14, 955–977 (1965).
Borgaonkar, D. S., Bias, W. B., Scott, G. I., Wadia, R. S., Borkowf, S. P.: IgA and abnormal chromosome 18. Lancet I, 206–207 (1969).
—, Scott, C. I.: Ring chromosome 18. Birth defects. Original articles series 5, No 5, 158–159 (1969).
DuPraw, E. J.: The ultrastructural of metaphase chromosomes. In: DNA and chromosomes, p. 132–171. New York: Holt, Rinehart and Winston, Inc. 1970.
—, Bahr, G. F.: The arrangement of DNA in human chromosomes as investigated by quantitative electron microscopy. Acta eytol. (Balt.) 13, 188–209 (1969).
Golomb, H. M., Bahr, G. F.: Dry mass and fiber evaluation of human chromosomes; normal and abnormal. Abstract, 60th annual meeting of International Academy of Pathology, Montreal, Canada (1971 a).
—: Scanning electron microscopic observations of the surface structure of isolated human chromosomes. Science 171, 1024–1026 (1971b).
- -: Analysis of an isolated metaphase plate by quantitative electron microscopy. Exp. Cell Res. (in press, 1971 c).
- - Borgaonkar, D. S.: Analysis of human chromosomal variants by quantitative electron microscopy. I. Group D chromosome with giant satellites. Genetics (in press, 1971).
Sinclair, J. H., Stevens, B. J.: Circular DNA filaments from mouse mitochondria. Proc. nat. Acad. Sci. (Wash.) 56, 508–514 (1966).
Sober, H. A. (Ed.): Handbook of biochemistry. Cleveland, Ohio: The Chemical Rubber Co. 1968.
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Preliminary presentation of this study was made (Golomb and Bahr, 1971a).
This study has been supported by American Cancer Society Grant No. P259-j.
The opinions or assertions contained herein are the private views of authors and are not to be construed as official or as reflecting the views of the department of the Army or the Department of Defense.
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Grolomb, H.M., Bahr, G.F. & Borgaonkar, D.S. Analysis of human chromosomal variants by quantitative electron microscopy. Chromosoma 34, 94–98 (1971). https://doi.org/10.1007/BF00285519
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DOI: https://doi.org/10.1007/BF00285519