Abstract
Differential staining of sister chromatids in male mouse meiotic cells was achieved by continuous infusion with BrdU from the tail vein followed by Giemsa staining (the FPG technique of Perry and Wolff, 1974). Analysis of 341 bivalents including XY, and 21 univalents reveals that: (1) visible crossing over coincided exactly with the site of chiasmata; (2) no evidence was obtained in support of chiasma terminalisation; (3) an anomalous type of crossing over was found in the monochiasmatic bivalents, which could not be explained by the conventional hypothesis for crossing over; (4) some of the terminally associated bivalents might be achiasmatic, and univalents might have originated from such bivalents; (5) in XY bivalents, sister chromatid association was between lightly and darkly stained chromatids, suggesting a lack of crossing over and the existence of other genetic controls for this association; (6) during meiosis sister chromatid exchanges (SCEs) might be less frequent than crossovers.
Similar content being viewed by others
References
Allen, J.W., Latt, S.A.: In vivo BrdU-33258 Hoechst analysis of DNA replication kinetics and sister chromatid exchange formation in mouse and meiotic cells. Chromosoma (Berl.) 58, 325–340 (1976)
Allen, J.W., Shuler, C.F., Mendes, R.W., Latt, S.A.: A simplified technique for in vivo analysis of sister-chromatid exchanges using 5-bromodeoxyuridine tablets. Cytogenet. Cell Genet. 18, 231–237 (1977)
Allen, J.W., Shuler, C.F., Latt, S.A.: Bromodeoxyuridine tablet methodology for in vivo studies of DNA synthesis. Somatic Cell Genet. 4, 393–405 (1978)
Böök, J.A., Kjessler, B.: Meiosis in the human male. Cytogenet. 3, 143–147 (1964)
Craig-Cameron, T., Jones, G.H.: The analysis of exchanges in tritium labelled meiotic chromosomes. 1. Schistocerca gregaria. Heredity 25, 223–232 (1970)
Darlington, C.D.: Chromosome behaviour and structural hybridity in the Tradescantiae. J. Genet. 21, 207–286 (1929)
Ford, C.E., Hamerton, J.L.: The chromosomes of man. Nature (Lond.) 178, 1020–1023 (1956)
Fox, D.P.: The control of chiasma distribution in the locust, Schistocerca gregaria (Forskål). Chromosoma (Berl.) 43, 289–328 (1973)
Hultén, M.: Chiasma distribution at diakinesis in the normal human male. Hereditas (Lund) 76, 55–78 (1974)
John, B.: Myths and mechanisms of meiosis. Chromosoma (Berl.) 54, 295–325 (1976)
Jones, G.H.: The analysis of exchanges in tritium-labelled meiotic chromosomes. Chromosoma (Berl.) 34, 367–382 (1971)
Jones, G.H.: A test for early terminalisation of chiasmata in diplotene spermatocytes of Schistocerca gregaria. Chromosoma (Berl.) 63, 287–294 (1977)
Jones, G.H.: Giemsa C-banding of rye meiotic chromosomes and the nature of “terminal” chiasmata. Chromosoma (Berl.) 66, 45–57 (1978)
Jones, G.H., Tease, C.: Analysis of exchanges in differentially stained meiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining. III. A test for chromatid interference. Chromosoma (Berl.) 73, 85–91 (1979)
Kanda, N., Kato, H.: A simple technique for in vivo observation of SCE in mouse ascites tumor and spermatogonial cells. Exp. Cell Res. 118, 431–434 (1979a)
Kanda, N., Kato, H.: In vivo sister chromatid exchange in cells of various organs of the mouse. Chromosoma (Berl.) 74, 299–305 (1979b)
Kato, H.: Spontaneous and induced sister chromatid exchanges as revealed by the BUdR-labelling method. Int. Rev. Cytol. 49, 55–97 (1977)
Kofman-Alfaro, S., Chandley, A.C.: Meiosis in the male mouse. An autoradiographic investigation. Chromosoma (Berl.) 31, 404–420 (1970)
Loidl, J.: C-band proximity of chiasmata and absence of terminalisation in Allium flavum (Liliaceae). Chromosoma (Berl.) 73, 45–51 (1979)
Luthardt, F.W., Palmer, C.G., Yu, P.-L.: Chiasma and univalent frequencies in aging female mice. Cytogenet. Cell Genet. 12, 68–79 (1973)
Monesi, V.: Autoradiographic study of DNA synthesis and the cell cycle in spermatogonia and spermatocytes of mouse testis using tritiated thymidine. J. Cell Biol. 14, 1–18 (1962)
Peacock, W.J.: Replication, recombination, and chiasmata in Goniaea australasiae. Genetics 65, 593–617 (1970)
Perry, P., Wolff, S.: New Giemsa method for the differential staining of sister chromatids. Nature (Lond.) 251, 156–158 (1974)
Polani, P.E., Jagiello, G.M.: Chiasmata, meiotic univalents, and in relation to aneuploid imbalance in mice. Cytogenet. Cell Genet. 16, 505–529 (1976)
Polani, P.E., Crolla, J.A., Seller, M.J., Moir, F.: Meiotic crossing over exchange in the female mouse visualised by BUdR substitution. Nature (Lond.) 278, 348–349 (1979)
Sasaki, M., Makino, S.: The meiotic chromosomes of man. Chromosoma (Berl.) 16, 637–651 (1965)
Schneider, E.L., Chaillet, J.R., Tice, R.R.: In vivo BUdR labeling of mammalian chromosomes, Exp. Cell Res. 100, 396–399 (1976)
Shaw, D.D.: Genetic and environmental components of chiasma control. Chromosoma (Berl.) 34, 281–301 (1971)
Shaw, D.D., Knowles, G.R.: Comparative chiasma analysis using a computerised optical digitiser. Chromosoma (Berl.) 59, 103–129 (1976)
Solari, A.J.: The behaviour of the XY pair in mammals. Int. Rev. Cytol. 38, 273–317 (1974)
Sumner, A.T.: A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75, 304–306 (1972)
Sybenga, J.: Meiotic configurations. Monographs on theoretical and applied genetics 1. Berlin, Heidelberg, New York: Springer 1975
Taylor, J.H.: Distribution of tritium-labeled DNA among chromosomes during meiosis. J. Cell Biol. 25, 57–67 (1965)
Tease, C.: Cytological detection of crossing-over in BUdR substituted meiotic chromosomes using the fluorescent plus Giemsa technique. Nature (Lond.) 272, 823–824 (1978)
Tease, C., Jones, G.H.: Analysis of exchanges in differentially stained meiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining. I. Crossover exchanges in monochiasmate bivalents. Chromosoma (Berl.) 69, 163–178 (1978)
Tease, C., Jones, G.H.: Analysis of exchanges in differentially stained meiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining. II. Sister chromatid exchanges. Chromosoma (Berl.) 73, 75–84 (1979)
Wolff, S.: Sister chromatid exchange. Ann. Rev. Genet. 11, 183–201 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kanda, N., Kato, H. Analysis of crossing over in mouse meiotic cells by BrdU labelling technique. Chromosoma 78, 113–121 (1980). https://doi.org/10.1007/BF00291910
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00291910