Abstract
Meiosis of T70H/+, Ts(113)70H translocation trisomic male mice has been studied using C-banded preparations and 3H-thymidine autoradiography of the first meiotic division. Epididymal sperm counts and sperm morphology scores were also collected. As reported earlier, at the first meiotic division the translocation involved chromosomes 1, 13, 131 and 113 (twice) formed mainly three multivalent configurations: Chain III+II, CIV+I and CV. — The autoradiographic study indicated an abnormal, precocious spiralization pattern for the chromatin in CIV+I primary spermatocytes. These cells, occurring together with the CIII+II and CV configurations in recognizable groups, usually descending from single spermatogonial stem cells, are delayed through meiotic prophase. Both delay and disturbed chromosome spiralization in these cells are attributed to the uniform association of the univalent (I) chromosome 113 with the sex chromosomes during pachytene. Primary spermatocytes of the CIV+I configuration and those carrying a CV take longer to develop from metaphase I into secondary spermatocytes than does the CIII+II type. — In T70H tertiary trisomics with a similar chromosome imbalance, the majority of primary spermatocytes degenerates during the diakinesis-metaphase I stages of meiosis. Fertility is low in contrast to the translocation trisomics. Comparison between the two types leads to the conclusion, that trisomy per se reduces the size of the testes and that the univalent containing CIV+I primary spermatocytes, contrary to the almost uniformly 113 univalent carrying spermatocytes of the T70H tertiary trisomics are rescued by the neighbouring CIII+II and CV carrying cells to form normal secondary spermatocytes and morphologically normal sperm.
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References
Boer, P. de: Fertile tertiary trisomy in the mouse (Mus musculus). Cytogenet. Cell Genet. 12, 435–442 (1973)
Boer, P. de: Male meiotic behaviour and male and female litter size in mice with the T(2;8)26H and T(1;13)70H reciprocal translocations. Genet. Res., Camb. 27, 369–387 (1976)
Boer, P. de, Beek, M.E.A.B. van: Meiosis of T70H translocation trisomic male mice I. Meiotic configurations and segregation. Chromosoma (Berl.) 87, 303–313 (1982)
Boer, P. de, Branje, H.E.B.: Association of the extra chromosome of tertiary trisomic male mice with the sex chromosomes during first meiotic prophase, and its significance for impairment of spermatogenesis. Chromosoma (Berl.) 73, 369–379 (1979)
Boer, P. de, Groen, A.: Fertility and meiotic behaviour of male T70H tertiary trisomics of the mouse (Mus musculus). A case of preferential telomeric meiotic pairing in a mammal. Cytogenet. Cell Genet. 13, 489–510 (1974)
Boer, P. de, Hoeven, F.A. van der, Chardon, J.A.P.: The production, morphology, karyotypes and transport of spermatozoa from tertiary trisomic male mice and the consequences for egg fertilization. J. Reprod. Pert. 48, 249–256 (1976)
Boer, P. de, Searle, A.G.: Workshop on chromosomal aspects of male sterility in mammals. Summary and synthesis. J. Reprod. Pert. 60, 257–265 (1980)
Bruce, W.R., Furrer, R., Wyrobek, A.J.: Abnormalities in the shape of murine sperm after acute testicular X-irradiation. Mutation Res. 23, 381–386 (1974)
Bryan, J.H.D.: An eosin-fast green-naphtol yellow mixture for differential staining of the cytologic components in mammalian spermatozoa. Stain Technol. 45, 213–236 (1970)
Carr, D.H., Walker, J.E.: Carbol fuchsin as a stain for mammalian chromosomes. Stain Technol. 36, 233–236 (1961)
Dym, M., Fawcett, D.W.: Further observations on the numbers of spermatogonia, spermatocytes and spermatids joined by intercellular bridges in mammalian spermatogenesis. Biol. Reprod. 4, 195–215 (1971)
Evans, E.P., Breckon, C., Ford, C.E.: An air-drying method for meiotic preparations from mammalian testes. Cytogenetics 3, 289–294 (1964)
Kofman-Alfaro, S., Chandley, A.C.: Meiosis in the male mouse, an autoradiographic investigation. Chromosoma (Berl.) 31, 404–420 (1970)
Mittwoch, U.: The difficulties of becoming a father (abstract). Cytogenet. Cell Genet. 27, 209 (1980)
Nijhoff, J.H., Boer, P. de: A first exploration of a Robertsonian translocation heterozygote in the mouse for its usefulness in cytological evaluation of radiation-induced meiotic autosomal non-disjunction. Mutation Res. 61, 77–86 (1979)
Nijhoff, J.H., Boer, P. de: Radiation-induced meiotic autosomal non-disjunction in male mice. The effects of low doses of fission neutrons and X-rays in Meiosis I and II of a Robertsonian translocation heterozygote. Mutation Res. 72, 431–446 (1980)
Oakberg, E.F.: A description of spermatogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am. J. Anat. 99, 391–409 (1956)
Searle, A.G., Beechey, C.V.: Sperm-count, egg-fertilization and dominant lethality after X-irradiation of mice. Mutation Res. 22, 63–72 (1974)
Sumner, A.T.: A simple technique for demonstrating centromeric heterochromatin. Exptl. Cell Res. 75, 304–306 (1972)
Sybenga, J.: Meiotic configurations. Springer, Berlin, Heidelberg, New York (1975)
Zwanenburg, T.S.B., Boer, P. de, Stam, P.: Clonal analysis of radiation-induced translocations in stem cell spermatogonia of normal and T70H translocation heterozygous mice. Mutation Res. 83, 207–219 (1981)
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de Boer, P., Speed, R.M. Meiosis of T70H translocation trisomic male mice. Chromosoma 87, 315–325 (1982). https://doi.org/10.1007/BF00327633
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DOI: https://doi.org/10.1007/BF00327633