Abstract
A molecular karyotype of the corn smut-inducing fungus Ustilago maydis was prepared using orthogonalfield-alternation gel electrophoresis (OFAGE). At least 20 chromosome-sized DNAs ranging from approximately 300 kb to the maximum limit of resolution of this system were identified in haploid cells of a widely used strain. Although general features of the banding pattern of chromosome-sized DNAs were conserved between strains, no two strains had identical karyotypes, indicating that considerable chromosome length polymorphism exists in this species. This polymorphism was seen in both laboratory strains as well as more recent isolates from nature. Length variation in apparently identical chromosomes was usually small, but was occasionally significant. In one strain Southern DNA hybridization analysis suggested the occurrence of a stable large-scale, inter-chromosomal exchange which had given rise to two novel chromosomes.
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References
Amasino RM (1986) Acceleration of nucleic acid hybridization rate by polyethylene glycol. Anal Biochem 152:304–307
Anand R (1986) Pulsed field gel electrophoresis: a technique for fractionation of large DNA molecules. Trends Genet 23:278–283
Banks GR (1983) Transformation of Ustilago maydis by a plasmid containing yeast 2-μ DNA. Curr Genet 7:73–77
Carle GF, Olson MV (1984) Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res 12:5647–5664
Carle GF, Olson MV (1985) An electrophoretic karyotype for yeast. Proc Natl Acad Sci USA 82:3756–3760
Christensen JJ (1963) Corn smut caused by Ustilago maydis. Monograph Number 2. Am Phytopathol Soc
Corcoran LM, Forsyth KP, Bianco AE, Brown GV, Kemp DJ (1986) Chromosome size polymorphisms in Plasmodium falciparum can involve deletions and are frequent in natural parasite populations. Cell 44:87–95
Durnam DM, Perrin F, Gannon F, Palmiter RD (1980) Isolation and characterization of the mouse metallothionein I gene. Proc Natl Acad Sci USA 77:6511–6515
Forster H, Kinscherf TG, Leong SA, Maxwell DP (1987) Molecular analysis of the mitochondrial genome of Phytophthora. Curr Genet 12:215–218
Free SJ, Rice PW, Metzenberg RL (1979) Arrangement of genes coding for ribosomal ribonucleic acids in Neurospora crassa. J Bacteriol 137:1219–1226
Garber ED, Day AW (1985) Genetic mapping of a phytopathogenic basidiomycete, Ustilago violacea. Bot Gaz 146:449–459
Garger SJ, Griffith OM, Grill LK (1983) Rapid purification of plasmid DNA by a simple centrifugation in a two step cesium chloride-ethidium bromide gradient. Biochem Biophys Res Commun 117:835–842
Garvey EP, Santi DV (1986) Stable amplified DNA in drug-resistant Leishmania exists as extrachromosomal circles. Science 233:535–540
Gibson WC, Miles MA (1986) The karyotype and ploidy of Trypanosoma cruzi. EMBO J 5:1299–1305
Holliday R (1974) Ustilago maydis. In: King RC (ed) Handbook of genetics, vol I. Plenum Press, New York, pp 575–595
Holliday R, Taylor SY, Kmiec EB, Holloman WK (1984) Biochemical characterization of rec 1 mutants and the genetic control of recombination in Ustilago maydis. Cold Spring Harbor Symp Quant Biol XLIX: 669–673
Kinscherf TG, Leong SA (1987) Ustilago maydis molecular karyotype. In: O'Brien SJ (ed) Genetic maps, vol 4. Cold Spring Harbor Laboratory Press, New York, pp 346–347
Kmiec EB, Holloman WK (1986) Homologous pairing of DNA molecules by Ustilago Rec 1 protein is promoted by sequences of Z-DNA. Cell 44:545–554
Kmiec EB, Kroeger P, Holliday R, Holloman W (1984) Homologous pairing promoted by Ustilago Rec 1 protein. Cold Spring Harbor Symp Quant Biol XLIX:675–682
Leong SA, Wang J, Budde A, Holden D, Kinscherf T, Smith T (1987) Molecular strategies for the analysis of the interaction of Ustilago maydis and maize. In: Arntzen C, Ryan C (eds) Molecular strategies for crop protection. Alan R. Liss, New York, pp 95–106
Magee BB, Magee PT (1987) Electrophoretic karyotypes and chromosome numbers in Candida species. J Gen Microbiol 133:425–430
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York
May G, Gambino J, Weatherbee J, Morris R (1985) Identification and functional analysis of β-tubulin genes by site-specific integrative transformation of Aspergillus nidulans. J Cell Biol 101:712–719
Ng R, Abelson J (1980) Isolation and sequence of the gene for actin in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 77:3912–3916
Schwartz D, Cantor CR (1984) Separation of yeast-chromosomesized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
Smith CL, Matsumoto T, Niwa O, Klco S, Fan J, Yanagida M, Cantor RC (1987) An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field electrophoresis. Nucleic Acids Res 15:4481–4489
Sutherland GR, Hecht F (1985) Fragile sites on human chromosomes. Oxford University Press, Oxford
Van der Ploeg LHT, Smits M, Ponnudurai T, Vermeulen A, Menwissen JHE Th, Langsley G (1985) Chromosome-sized DNA molecules of Plasmodium falciparum. Science 229:656–661
Wang J, Holden D, Leong SA (1988) Gene transfer system for the phytopathogenic fungus Ustilago maydis. Proc Natl Acad Sci USA 85:865–869
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Kinscherf, T.G., Leong, S.A. Molecular analysis of the karyotype of Ustilago maydis . Chromosoma 96, 427–433 (1988). https://doi.org/10.1007/BF00303036
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DOI: https://doi.org/10.1007/BF00303036