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Polymorphism of coagulation factor XIII B subunit: Further occurrence of FXIIIB*15 in Japanese and phenotyping in bloodstains

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Summary

The polymorphism of FXIIIB was investigated in 555 unrelated Japanese individuals using isoelectric focusing and immunoblotting. Five common phenotypes and a rare variant type FXIIIB 15-3 were observed. The allele frequencies were FXIIIB*1 = 0.3063, FXIIIB*2 = 0.0162, FXIIIB*3 = 0.6766 and FXIIIB*15 = 0.0009. Phenotyping was also possible from bloodstains stored at 37°C for up to 4 months and from bloodstains stored at room temperature and at 4°C for over 6 months. The FXIIIB system can provide a new powerful genetic marker for the medicolegal grouping of bloodstains.

Zusammenfassung

Der Polymorphismus des FXIIIB wurde bei 555 unverwandten Japanern mit Hilfe der isoelektrischen Fokussierung und anschließendem Immunoblotting untersucht. Fünf allgemein vorkommende Phänotypen und die seltene Variante FXIIIB 15-3 wurden beobachtet. Die Allel-Frequenzen waren FXIIIB = 0,3063, FXIIIB2 = 0,0162, FXIIIB3 = 0,6766 und FXIIIB15 = 0,0009. Die Bestimmung der Phänotypen war auch an Blutspuren mit folgenden Lagerungsbedingungen möglich: Bei 37°C über einen Zeitraum bis zu 4 Monate, bei Raumtemperatur und bei 4°C länger als 6 Monate. Das FXIIIB-System kann einen neuen, aussagekräftigen genetischen Marker für gerichtsmedizinische Blutspurenuntersuchungen darstellen.

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Authors and Affiliations

  1. Department of Legal Medicine, Yamanashi Medical University, Tamaho, 409-38, Yamanashi-ken, Japan

    N. Komatsu, A. Kido, Y. Kimura & M. Oya

  2. Forensic Science Laboratory, Yamanashi Prefectural Police Headquarters, Nakakogawara, 400, Kofu-shi, Yamanashi-ken, Japan

    Y. Dobashi & K. Hashimoto

Authors
  1. N. Komatsu
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  2. A. Kido
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  3. Y. Kimura
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  4. M. Oya
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  5. Y. Dobashi
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  6. K. Hashimoto
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Komatsu, N., Kido, A., Kimura, Y. et al. Polymorphism of coagulation factor XIII B subunit: Further occurrence of FXIIIB*15 in Japanese and phenotyping in bloodstains. Int J Leg Med 104, 317–319 (1992). https://doi.org/10.1007/BF01369549

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  • DOI: https://doi.org/10.1007/BF01369549

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