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Errors in ABO typing of blood stains using PCR

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Abstract

Genotypes of the ABO blood group system were investigated using a multiplex PCR and subsequent restriction enzyme digestion on experimental blood stains. Differences were found when typing blood between the PCR and serological methods and one blood sample, typed as B with the agglutination test was classified as AB using the method described here. The subsequent sequencing procedure revealed the genotype to be BB. Methodological causes for errors in typing which should be taken into consideration are discussed.

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Authors and Affiliations

  1. Institut für Rechtsmedizin der Justus-Liebig Universität, Frankfurter Strasse 58, 35392 Giessen, Germany Tel.: +49-641-9941411; Fax: +49-641-9941419, Germany

    P. F. Ringel & G. Weiler

  2. Institut für Klinische Immunologie und Transfusionsmedizin der Justus-Liebig Universität, Langhansstrasse 7, 35385 Giessen, Germany, Germany

    G. Bein

Authors
  1. P. F. Ringel
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  2. G. Weiler
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  3. G. Bein
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Received: 28 June 1999 / Received in revised form: 23 September 1999

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Ringel, P., Weiler, G. & Bein, G. Errors in ABO typing of blood stains using PCR. Int J Leg Med 113, 352–355 (2000). https://doi.org/10.1007/s004149900127

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  • DOI: https://doi.org/10.1007/s004149900127

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