Abstract
By immunohistochemistry of rat brain in conjunction with a specific antibody against 5-hydroxytryptophan (5HTP), we examined immunoreactivity to 5HTP in neurons, from which 5-hydroxytryptamine (5HT; serotonin) was depleted by reserpine treatment. The distribution patterns of 5HTP-positive neurons overlapped with those of 5HT neurons. Treatment with reserpine (5 mg/kg, 90 min before death) caused a complete suppression of 5HT-positive staining, but 5HTP-immunostaining remained in perikarya of the nuclei raphe dorsalis, centralis superior and obscurus. Treatment with reserpine (25 mg/kg, 90 min before death) suppressed the 5HTP-immunoreaction in certain perikarya (e.g. of the nucleus raphe dorsalis) and fibres; however, 5HTP-immunostaining remained in perikarya of the nuclei centralis superior and raphe obscurus. This suggests that these neurons synthesize more 5HTP by a process which appears to be stimulated by reserpine.
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References
Andén NE, Henning M (1966) Adrenergic nerve function, noradrenaline level and noradrenaline uptake in cat nictitating membrane after reserpine treatment. Acta Physiol Scand 67:498–504
Carlsson A (1965) Drugs which block the storage of 5-hydroxytryptamine and related amines. In: Erspamer V (ed) Handbuch der experimentellen Pharmakologie, vol 19. Springer, Berlin Heidelberg New York, pp 529–592
Carlsson A Lindqvist M (1978) Effect of reserpine on monoamine synthesis and on apparent dopaminergic receptor sensitivity in rat brain. In: Haber B, Aprison MH (eds) Neuropharmacology and behavior. Plenum Press, New York, pp 89–102
Fujimiya M, Kimura H, Maeda T (1991) Serotonin-containing epithelial cells in rat duodenum. I. Quantitative morphometric study of the distribution density. Histochemistry 95:217–224
Geffard M, Touret M, Kitahama K (1987) First characterization of 5-hydroxytryptophan in rat brain by using specific antibodies. Brain Res 426:191–196
Hamon M, Glowinski J (1974) Regulation of serotonin synthesis. Life Sci 15:1533–1548
Hjörth S (1992) Acute reserpine treatment increases rat brain serotonin synthesis via a nerve impulse-dependent mechanism. J Neurochem 58:772–775
Hjörth S, Magnusson T (1988) The 5-HT1A receptor agonist, 8-OH-DPAT, preferentially activates cell body 5HT autoreceptors in rat brain in vivo. Naunyn Schmied Arch Pharmacol 338:463–471
Kitahama K, Mons N, Okamura H, Jouvet M, Geffard M (1988) Endogenous L-DOPA, its immunoreactivity in neurons of midbrain and its projection fields in the cat. Neurosci Lett 95:47–52
Saner A, Pletscher A (1978) Enhancement of 5-hydroxytryptamine synthesis in brain by monoamine-depleting drugs. J Pharm Pharmacol 30:115–117
Steinbusch HWM (1984) Serotonin-immunoreactive neurons and their projections in the CNS. In: Björklund A, Hökfelt T, Kuhar MJ (eds) Handbook of chemical neuroanatomy, vol 3. Elsevier Science Publishers, Amsterdam, pp 68–125
Tappaz M, Pujol JF (1980) Estimation of the rate of tryptophan hydroxylation in vivo: a sensitive microassay in discrete rat brain nuclei. J Neurochem 34:933–940
Tomida Y, Kimura H (1987) Immunohistochemical and biochemical studies of substances with taurine-like immunoreactivity in the brain. Acta Histochem Cytochem 20:31–40
Touret M, Kitahama K, Geffard M, Jouvet M (1987) 5-Hydroxytryptophan (5HTP)-immunoreactive neurons in the rat brain tissues. Neurosci Lett 80:263–267
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Fujimiya, M., Nakazawa, M., Maeda, T. et al. Effect of reserpine on 5-hydroxytryptophan (5HTP)-immunoreactive neurons in the rat brain. Histochemistry 101, 21–26 (1994). https://doi.org/10.1007/BF00315827
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DOI: https://doi.org/10.1007/BF00315827