Skip to main content
Log in

The occurrence of neutral and acidic mucins in the reproductive tract of the laying hen

  • Published:
Histochemie Aims and scope Submit manuscript

Summary

The tissues of the domestic hen's reproductive tract have been shown to contain both acidic and neutral mucins. The presence of an egg in the isthmus was always accompanied with an increase in the concentration in the isthmus secretory epithelium of PAS-positive mucins without free acidic groups. Staining of uterine tissue for strongly acidic groups with basic dyes was greater when an egg had been present in the shell gland at the time of death and the same was true for tissues of the upper oviduct. The results are discussed with special reference to the periodic secretion of organic mammillary core and egg shell mucins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baker, J. R., and D. A. Balch: A study of the organic material of the hen's egg. Biochem. J. 82, 352–361 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fisher, E. R., and R. D. Lillie: The effect of methylation on basophilia. J. Histochem. Cytochem. 2, 81–87 (1954).

    Article  CAS  PubMed  Google Scholar 

  • Gottschalk, A.: Glycoproteins. Their composition structure and function. Ed. A. Gottschalk, B. B. A. Library, vol. 5, p. 282. Amsterdam: Elsevier Publishing Co. 1966.

    Google Scholar 

  • McCallion, D. J.: A cytological and cytochemical study of the shell gland of the domestic hen. Canad. J. Zool. 31, 577–589 (1953).

    Article  CAS  Google Scholar 

  • Moran, T., and H. P. Hale: Physics of the hen's egg. 1. Membranes in the egg. J. exp. Biol. 13, 35–40 (1936).

    Google Scholar 

  • Mowey, R. W.: Improved procedure for the staining of acidic polysaccharides by Müller's collodial (hydrous) ferric oxide and its combination with the Feulgen and periodic acidschiff reactions. Lab. Invest. 7, 566–576 (1958).

    Google Scholar 

  • —: The special value of the methods that color both acidic and vicinal hydroxyl groups in the histochemical study of mucins with revised directions for the colloidal iron stain, the use of alcian blue 8 GX and their combinations with the periodic acid-schiff reactions. Ann. N.Y. Acad. Sci. 106, 402–423 (1963).

    Google Scholar 

  • Robinson, D. S., and N. R. King: Carbonic anhydrase and formation of the hen's egg shell. Nature (Lond.) 199, 497–498 (1963).

    Article  CAS  Google Scholar 

  • —: Mucopolysaccharides of an avian egg shell membrane. J. roy. micr. Soc. 88, 13–22 (1968).

    Article  CAS  PubMed  Google Scholar 

  • — and D. J. Bowen: Neutral and acidic mucins in the reproductive tract of the laying hen. Proc. J. micr. Soc. 1, part 4, 214 (1966).

    Google Scholar 

  • Romanoff, A. L., and A. J. Romanoff: The avian egg. New York: Wiley (1949).

    Google Scholar 

  • Scott, J. E., and J. Dorling: Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions. Histochemie 5, 221–233 (1965).

    Article  CAS  PubMed  Google Scholar 

  • - - and G. Quintabelli: Differential staining of acid glycosaminoglycans by alcian blue in salt solutions. Biochem. J. 91, 4p (1964).

    Google Scholar 

  • Spicer, S. S., and J. Duvenci: Histochemical characteristics of mucopolysacoharides in salivary and exorbital lacrimal glands. Anat. Rec. 149, 333–358 (1964).

    Article  CAS  PubMed  Google Scholar 

  • — and D. B. Meyer: Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin-alcian blue staining. Amer. J. clin. Path. 33, 453–460 (1960).

    CAS  Google Scholar 

  • — R. C. Swarm, and H. J. Burtner: Comparison of basophilia with S 35label in normal and methylated mucopolysaccharides. Lab. Invest. 10, 256–264 (1961).

    CAS  Google Scholar 

  • Taylor, T. G., and F. Hertelendy: Parallel distribution of calcium and citric acid in the oviduct of the hen. Nature (Lond.) 187, 244–245 (1960).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Robinson, D.S., King, N.R. & Bowen, D.J. The occurrence of neutral and acidic mucins in the reproductive tract of the laying hen. Histochemie 13, 97–104 (1968). https://doi.org/10.1007/BF00266574

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00266574

Keywords

Navigation