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Zymograms and histochemistry of non-specific esterase in the salivary glands

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Summary

Zymogramic analysis of esterase in the mouse, rat and guinea-pig salivary glands was undertaken and demonstrated species and organ specificities of esterase with electrophoretic method. Salivary glands esterase was classified into A, B and C types based on the electrophoretic mobility. Mouse submandibular gland had the most complicated pattern, while guinea-pig showed the simpliest patterns which was devoid of B type of esterase. Rat salivary glands exhibited rather regular patterns. Similar zymogram patterns were obtained with many kinds of ester compounds, that is simple and substituted naphthol esters and indoxyl derivatives. The tests of inhibition and activation for esterase activity was obtained. Histochemical properties applied to inhibitor test in the esterase zymogram patterns showed no marked differences between ducts and acini.

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References

  • Augustinsson, K. B.: Electrophoresis studies on blood plasma esterases. Acta chem. scand. 13, 571–592 (1959).

    Google Scholar 

  • —: Multiple forms of esterase in vertebrate blood plasma. Ann. N.Y. Acad. Sci. 94, 844–860 (1961).

    Google Scholar 

  • Barron, K. D., T. Bernsohn, and A. R. Hess: Separation and properties of human brain esterases. J. Histochem. Cytochem. 11, 139–156 (1963).

    Google Scholar 

  • Burstone, M. S.: Esterases of the salivary glands. J. Histochem. Cytochem. 4, 130–139 (1956).

    Google Scholar 

  • Cahn, R. D., N. O. Kaplan, L. Levine, and E. Zwilling: Nature and development of lactic dehydrogenases. Science 136, 962–969 (1962).

    Google Scholar 

  • Carvalheira, A., and A. G. E. Pearse: Comparative cytochemistry of C cell esterases in the mammalian hyroid-parathyroid complex. Histochemie 8, 175–182 (1967).

    Google Scholar 

  • Erankö, O., M. Härkönen, A. Kokko, and L. Räisänen: Histochemical and starch gel electrophoretic characterization of desmo-and lyoesterases in the sympathetic and spinal ganglia of the rat. J. Histochem. Cytochem. 8, 570–581 (1964).

    Google Scholar 

  • Garrett, J. R.: Some observations on human submandibular salivary glands. Proc. roy.Soc. Med. 55, 488–491 (1962).

    Google Scholar 

  • —: The innervation of normal submandibular and parotid glands. Demonstration by cholinesterase histochemistry. Catecholamine fluorecence and electron microscopy. Arch. oral Biol. 12, 1417–1436 (1967).

    Google Scholar 

  • Holmes, R. S., and C. J. Masters: The developmental multiplicity and isoenzyme status of cavian esterases. Biochim. biophys. Acta (Amst.) 132, 379–399 (1967).

    Google Scholar 

  • — The developmental multiplicity and isoenzyme status of rat esterase. Biochim. biophys. Acta (Amst.) 146, 138–150 (1967).

    Google Scholar 

  • — A comparative study of the multiplcity of mamalian esterases. Biochim. biophys. Acta (Amst.) 151, 147–158 (1968).

    Google Scholar 

  • Kaplan, N. O., and M. M. Ciotii: Evolution and differentiation of dehydrogenases. Ann. N.Y. Acad. Sci. 94, 701–722 (1961).

    Google Scholar 

  • Kawakatsu, K., and M. Mori: Histochemical study of enzyme patterns in the human submaxillary gland. Histochemie 2, 393–401 (1962).

    Google Scholar 

  • Kawakatsu, K., K. Fujita, M. Fukuda, and T. Kawagoe: Histochemical studies of normal salivary glands. II. Distribution and localization of esterase in the salivary glands of experimental animals. J. Osaka Univ. dent. Soc. 4, 439–454 (1959).

    Google Scholar 

  • Komma, D. J.: Characteristics of the esterases of human cells grown in vitro. J. Histochem. Cytochem. 11, 621–623 (1963).

    Google Scholar 

  • Kreusser, E. H.: Non specific esterases in normal and neoplastic tissues of the syrian hamster, A zymogram study. Cancer Res. 26, 2181–2185 (1966).

    Google Scholar 

  • Markert, C. L.: The molecular basis for isoenzymes. Ann. N.Y. Acad. Sci. 151, 14–40 (1968).

    Google Scholar 

  • —, and R. L. Hunter: The distribution of esterases in mouse tissue. J. Histochem. Cytochem. 7, 42–49 (1959).

    Google Scholar 

  • —, and F. Moller: Multiple forms of enzymes: tissue, ontogenetic, and species specific patterns. Proc. nat. Acad. Sci. (Wash.) 45, 754–763 (1956).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry, theoretical and applied, chap. XVI. London: Churchill 1960.

    Google Scholar 

  • Raymond, S., and Y. I. Wang: Preparation and properties of acrylamide gel for use in electrophoresis. Analyt. Biochem. 1, 391–396 (1960).

    Google Scholar 

  • Snell, R. S.: The histochemical appearances of cholinesterase in the parotid salivary gland of the rat. Z. Zellforsch. 49, 330–338 (1959).

    Google Scholar 

  • -The innervation of the salivary gland. A study of cholinesterase in nerves. Histochemistry of cholinesterase Symposium, Basel. Bibl. Anat. 2, 97–110. Basel/New York: Karger 1960, 1961.

  • —, and J. R. Garrett: The distribution of cholinesterase in the submaxillary and sublingual salivary glands of the rat. J. Histochem. Cytochem. 5, 236–245 (1957).

    Google Scholar 

  • Tani, T.: Histochemical observation of hydrolytic enzymes in major salivary glands. J. Osaka Univ. dent. Sch. 5, 213–230 (1960).

    Google Scholar 

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Yoshimura, Y., Morishita, M., Mori, M. et al. Zymograms and histochemistry of non-specific esterase in the salivary glands. Histochemie 18, 302–313 (1969). https://doi.org/10.1007/BF00279881

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