Summary
Zymogramic analysis of esterase in the mouse, rat and guinea-pig salivary glands was undertaken and demonstrated species and organ specificities of esterase with electrophoretic method. Salivary glands esterase was classified into A, B and C types based on the electrophoretic mobility. Mouse submandibular gland had the most complicated pattern, while guinea-pig showed the simpliest patterns which was devoid of B type of esterase. Rat salivary glands exhibited rather regular patterns. Similar zymogram patterns were obtained with many kinds of ester compounds, that is simple and substituted naphthol esters and indoxyl derivatives. The tests of inhibition and activation for esterase activity was obtained. Histochemical properties applied to inhibitor test in the esterase zymogram patterns showed no marked differences between ducts and acini.
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References
Augustinsson, K. B.: Electrophoresis studies on blood plasma esterases. Acta chem. scand. 13, 571–592 (1959).
—: Multiple forms of esterase in vertebrate blood plasma. Ann. N.Y. Acad. Sci. 94, 844–860 (1961).
Barron, K. D., T. Bernsohn, and A. R. Hess: Separation and properties of human brain esterases. J. Histochem. Cytochem. 11, 139–156 (1963).
Burstone, M. S.: Esterases of the salivary glands. J. Histochem. Cytochem. 4, 130–139 (1956).
Cahn, R. D., N. O. Kaplan, L. Levine, and E. Zwilling: Nature and development of lactic dehydrogenases. Science 136, 962–969 (1962).
Carvalheira, A., and A. G. E. Pearse: Comparative cytochemistry of C cell esterases in the mammalian hyroid-parathyroid complex. Histochemie 8, 175–182 (1967).
Erankö, O., M. Härkönen, A. Kokko, and L. Räisänen: Histochemical and starch gel electrophoretic characterization of desmo-and lyoesterases in the sympathetic and spinal ganglia of the rat. J. Histochem. Cytochem. 8, 570–581 (1964).
Garrett, J. R.: Some observations on human submandibular salivary glands. Proc. roy.Soc. Med. 55, 488–491 (1962).
—: The innervation of normal submandibular and parotid glands. Demonstration by cholinesterase histochemistry. Catecholamine fluorecence and electron microscopy. Arch. oral Biol. 12, 1417–1436 (1967).
Holmes, R. S., and C. J. Masters: The developmental multiplicity and isoenzyme status of cavian esterases. Biochim. biophys. Acta (Amst.) 132, 379–399 (1967).
— The developmental multiplicity and isoenzyme status of rat esterase. Biochim. biophys. Acta (Amst.) 146, 138–150 (1967).
— A comparative study of the multiplcity of mamalian esterases. Biochim. biophys. Acta (Amst.) 151, 147–158 (1968).
Kaplan, N. O., and M. M. Ciotii: Evolution and differentiation of dehydrogenases. Ann. N.Y. Acad. Sci. 94, 701–722 (1961).
Kawakatsu, K., and M. Mori: Histochemical study of enzyme patterns in the human submaxillary gland. Histochemie 2, 393–401 (1962).
Kawakatsu, K., K. Fujita, M. Fukuda, and T. Kawagoe: Histochemical studies of normal salivary glands. II. Distribution and localization of esterase in the salivary glands of experimental animals. J. Osaka Univ. dent. Soc. 4, 439–454 (1959).
Komma, D. J.: Characteristics of the esterases of human cells grown in vitro. J. Histochem. Cytochem. 11, 621–623 (1963).
Kreusser, E. H.: Non specific esterases in normal and neoplastic tissues of the syrian hamster, A zymogram study. Cancer Res. 26, 2181–2185 (1966).
Markert, C. L.: The molecular basis for isoenzymes. Ann. N.Y. Acad. Sci. 151, 14–40 (1968).
—, and R. L. Hunter: The distribution of esterases in mouse tissue. J. Histochem. Cytochem. 7, 42–49 (1959).
—, and F. Moller: Multiple forms of enzymes: tissue, ontogenetic, and species specific patterns. Proc. nat. Acad. Sci. (Wash.) 45, 754–763 (1956).
Pearse, A. G. E.: Histochemistry, theoretical and applied, chap. XVI. London: Churchill 1960.
Raymond, S., and Y. I. Wang: Preparation and properties of acrylamide gel for use in electrophoresis. Analyt. Biochem. 1, 391–396 (1960).
Snell, R. S.: The histochemical appearances of cholinesterase in the parotid salivary gland of the rat. Z. Zellforsch. 49, 330–338 (1959).
-The innervation of the salivary gland. A study of cholinesterase in nerves. Histochemistry of cholinesterase Symposium, Basel. Bibl. Anat. 2, 97–110. Basel/New York: Karger 1960, 1961.
—, and J. R. Garrett: The distribution of cholinesterase in the submaxillary and sublingual salivary glands of the rat. J. Histochem. Cytochem. 5, 236–245 (1957).
Tani, T.: Histochemical observation of hydrolytic enzymes in major salivary glands. J. Osaka Univ. dent. Sch. 5, 213–230 (1960).
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Yoshimura, Y., Morishita, M., Mori, M. et al. Zymograms and histochemistry of non-specific esterase in the salivary glands. Histochemie 18, 302–313 (1969). https://doi.org/10.1007/BF00279881
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DOI: https://doi.org/10.1007/BF00279881