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A staining method of epoxy-embedded lymphatic and hemopoietic tissues in autoradiograms

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Summary

Autoradiograms of sections 0.8–1.0 μ thick of epoxy-embedded, glutaraldehyde and OsO4 fixed lymphatic and hemopoietic tissues were stained with 4% aqueous basic fuchsin at 70°C for 3 min, rinsed well and destained twice at 70°C for 1 min. A 1% methylene blue solution dissolved in 1% borax aqueous solution mixed with 1% aqueous azur II solution in the proportion of 1:1 was then allowed to act on the slide at 70°C for 1 min. The stain was rinsed well off slide and destained also twice at 70°C for 1 min.

The preparation was dried before applying a mounting medium (Eukitt) and cover glass. An Eukitt mounting prevented the stain from fading for at least 1 year. The method is simple, rapid and provides sharp contrast in autoradiography with well destained nucleal emulsion.

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Author notes

  1. K. Yamashita

    Present address: Department of Pathology, University of Hiroshima, Hiroshima, Japan

Authors and Affiliations

  1. Department of Pathology, University of Bonn, Bonn, Germany

    K. Yamashita & B. Helpap

  2. Pathologisches Institut, Venusberg, D-5300, Bonn 1, Federal Republic of Germany

    K. Yamashita & B. Helpap

Authors
  1. K. Yamashita
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  2. B. Helpap
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Additional information

This study was supported by Alexander von Humboldt-Foundation.

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Yamashita, K., Helpap, B. A staining method of epoxy-embedded lymphatic and hemopoietic tissues in autoradiograms. Histochemistry 40, 275–279 (1974). https://doi.org/10.1007/BF00501963

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  • DOI: https://doi.org/10.1007/BF00501963

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