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Histochemical and immunohistochemical properties of the CRF-Granules and other „Gomori-positive” substances of the rat

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Summary

In adrenalectomized rats, histochemical and immunohistochemical properties of the following secretion products have been investigated:

  1. 1.

    CRF-granules in the outer layer of the median eminence;

  2. 2.

    neurosecretory material (NSM) in the supraoptico-hypophysial system of the hypothalamus;

  3. 3.

    secretory granules in the TSH-cells of the anterior lobe of the hypophysis;

  4. 4.

    secretory granules in the ependymal cells of the subcommissural organ (SCO);

  5. 5.

    β-cell-granules in the islets of Langerhans in the pancreas.

All these substances are characterized by their stainability with the so-called “Gomorimethod”.

The experiments have included studies into:

  1. a)

    the extractability of the substances by various solvents;

  2. b)

    the digestability of the substances by pepsin or trypsin;

  3. c)

    their histochemically detectable content of disulfide groups, arginine and periodic acid-Schiff (PAS) reactive carbohydrates;

  4. d)

    their reaction with porcine-neurophysin-II-antibodies.

All substances exhibited a positive reaction for disulfide groups. Based on their solutility properties, their resistance to pepsin or trypsin, their respective content of PAS-reactive carbohydrates and their failure to react with anti-neurophysin serum the “Gomori-positive” granules in TSH-, SCO- and pancreatic β-cells can be distinguished from one another and from CRF- and neurosecretory granules. In contrast, CRF-granules and NSM showed identical properties.

Taking into consideration data from the biochemical and histochemical literature, the present findings suggest that CRF-granules and NSM consist of closely related biochemical substances.

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Authors and Affiliations

  1. Anatomisches Institut, Universität Bonn, Fed. Rep. Germany

    R. Bock, Th. Salland & P. E. Schwabedal

  2. Postgraduate School of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand

    W. B. Watkins

  3. Anatomisches Institut der Universität des Saarrlandes, D-665, Homburg/Saar

    R. Bock

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  1. R. Bock
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  2. Th. Salland
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  3. P. E. Schwabedal
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  4. W. B. Watkins
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Dedicated to Professor Dr. Drs. h.c. W. Bargmann on the occasion of his 70th birthday.

This work was supported by grants from the Landesamt für Forschung, Nordrhein-Westfalen (BRD) and from the Medical Research Council of New Zealand.

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Bock, R., Salland, T., Schwabedal, P.E. et al. Histochemical and immunohistochemical properties of the CRF-Granules and other „Gomori-positive” substances of the rat. Histochemistry 46, 81–105 (1976). https://doi.org/10.1007/BF02462734

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