Summary
The optimal conditions for the demonstration of arylsulphatase activity in the proximal convoluted tubule cells of the rat kidney were studied at light and electron microscopic level. 8-hydroxyquinoline sulphate, p-nitrophenyl sulphate and 2-hydroxy-5-nitrophenylsulphate were used as substrates and barium and lead as capturing ions. The effect of fixation, capturing ions, substrate concentration and pH was studied biochemically. The results of these biochemical studies were then verified histochemically. Finally a recommended method for the light and electron microscopic demonstration of arylsulphatase activity was presented.
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Hopsu-Havu, V.K., Arstila, A.U., Helminen, H.J. et al. Improvements in the method for the electron microscopic localization of arylsulphatase activity. Histochemie 8, 54–64 (1966). https://doi.org/10.1007/BF00279874
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DOI: https://doi.org/10.1007/BF00279874