Summary
The immunocytochemical localization of 17β-hydroxysteroid dehydrogenase (17β-HSD) in porcine testes was examined by applying an indirect-immunofluorescence method using an antiporcine testicular 17β-HSD antibody. Only the Leydig cells located in the interstitial tissue exhibited a positive immunoreaction for 17β-HSD: the germ cells and Sertoli cells located in the seminiferous tubules were entirely negative. These results suggest that, in porcine testis, the biosynthesis of testicular testosterone, the final step of which is the conversion of androstenedione to testosterone, takes place in the Leydig cells.
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Supported by grants from the Ministry of Education, Science, and Culture, Japan
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Kurosumi, M., Ishimura, K., Yoshinaga, T. et al. Immunocytochemical localization of 17β-hydroxysteroid dehydrogenase in porcine testis. Histochemistry 85, 287–289 (1986). https://doi.org/10.1007/BF00493478
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DOI: https://doi.org/10.1007/BF00493478