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GABAergic input of cholecystokinin-immunoreactive neurons in the hilar region of the rat hippocampus

An electron microscopic double immunostaining study

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Summary

Double immunostaining was performed for electron microscopy to analyze the synaptic connections between glutamate decarboxylase (GAD)-immunoreactive axons and cholecystokinin (CCK)-immunoreactive neurons in the hilar region of the rat hippocampal formation. Following immunostaining for CCK, the diaminobenzidine (DAB) reaction product was silver-intensified and gold-substituted. In a subsequent second immunostaining for GAD, the immunoreactive elements were labeled using a single DAB reaction. Electron microscopic analysis of the double-stained Vibratome sections demonstrated that the single DAB-labeled GAD-immunoreactive boutons form symmetrical synaptic connections on the soma and primary dendrites of the DAB-gold-labeled CCK-immunoreactive neurons.

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Authors and Affiliations

  1. Section of Neuroanatomy and Department of Obstetrics and Gynecology, Lale University, School of Medicine, 333 Cedar Street, P.O. Box 3333, 06510, New Haven, Connecticut, USA

    C. Leranth

  2. Institute of Anatomy, Johann-Wolfgang-Goethe University, Frankfurt am Main, Federal Republic of Germany

    M. Frotscher

Authors
  1. C. Leranth
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  2. M. Frotscher
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Leranth, C., Frotscher, M. GABAergic input of cholecystokinin-immunoreactive neurons in the hilar region of the rat hippocampus. Histochemistry 86, 287–290 (1987). https://doi.org/10.1007/BF00490260

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  • DOI: https://doi.org/10.1007/BF00490260

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