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Immunocytochemical localization of Vitamin A in the retina and pineal organ of the frog,Rana esculenta

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Summary

Vitamin A immunoreactive sites were studied in the retina and pincal organ of the frog,Rana esculenta, by the peroxidase antiperoxidase, avidin-biotinperoxidase and immunogold methods. Indark-adapted material, strong immunoreaction was found in the outer and inner segments of the photoreceptor cells of both retina and pineal organ, as well as in the pigment epithelium, retinal Müller cells and pineal ependymal cells. Inlight-adapted retina, cones and green (blue-sensitive) rods were immunopositive.

At the electron microscopic level, immunogold particles were found on the membranes of the photoreceptor outer segments as well as on the membranes of the endoplasmic reticulum and mitochondria. Individual retinal photoreceptor cells exhibited strong immunoreaction in the distal portion of the inner segment, the ciliary connecting piece and the electron-dense material covering the outer segment. In the pigment epithelium, the immunolabeling varied in intensity in the basal and apical cytoplasm and phagocytosed outer segments.

The immunocytochemical results indicate that retinoids (retinal, retinol and possibly retinoic acid) are present not only in the photoreceptor cells of the retina but also in those of the pineal organ. The light-dependent differences in the immunoreactivity of vitamin A underlines its essential role in the visual cycle of the photopigments. Our results suggest that the pineal ependyma plays a role comparable to that of the Müller cells and pigment epithelium of the retina with regard to the transport and storage of vitamin A. The presence of a retinoid in nuclei, mitochondria and cytoplasmic membranes suggests an additional role of vitamin A in other metabolic processes.

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Authors and Affiliations

  1. 2nd Department of Anatomy, and Neuroendocrine Laboratory of the Hungarian Academy of Sciences-Semmelweis University Medical School Joint Research Organization, Tüzoltó utca 58, H-1094, Budapest, Hungary

    I. Vigh-Teichmann, B. Vigh & A. Szél

  2. 1st Electron Microscopic Laboratory, Semmelweis University Medical School, Tüzoltó utca 58, H-1094, Budapest, Hungary

    A. Szél & P. Röhlich

  3. Department of Biochemistry, West Virginia University Medical Center, 26506, Morgantown, West Virginia, USA

    G. H. Wirtz

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  1. I. Vigh-Teichmann
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  5. G. H. Wirtz
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Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday

Supported by the Hungarian OTKA grant Nr. 1619 to B.V., and a grant from the Pardee Foundation to G.H.W.

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Vigh-Teichmann, I., Vigh, B., Szél, A. et al. Immunocytochemical localization of Vitamin A in the retina and pineal organ of the frog,Rana esculenta . Histochemistry 88, 533–543 (1988). https://doi.org/10.1007/BF00570321

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