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Distribution of a catecholamine-binding mechanism in rat brain

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Summary

The existence of characteristic regional differences in the binding capacity of tissue for catecholamines was demonstrated by incubating sections of rat brain with tritiated norepinephrine or epinephrine or dopamine, and by radioautography. Microscopically, catecholamine binding occurred diffusely in the neuropil, or in pericellular aggregates upon nerve cells, in a pattern highly suggestive of synaptic buttons. A neuroanatomical account of the regional distribution patterns of norepinephrine binding in rat brain showed similarity to the known regional distribution of norepinephrine. However, there were quantitative and qualitative differences between the distribution of the binding mechanism and that of norepinephrine. Minor differences were found between the binding of norepinephrine and epinephrine; little binding occurred for dopamine. The binding mechanism was not facilitated by ATP, and was resistant to reserpine.

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Authors and Affiliations

  1. Institute of Pathology, Western Reserve University, Cleveland, Ohio

    T. Ishii & R. L. Friede

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  1. T. Ishii
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  2. R. L. Friede
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This investigation was supported by Public Health Grant NB 6239 from the National Institute of Neurological Diseases and Blindness.

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Ishii, T., Friede, R.L. Distribution of a catecholamine-binding mechanism in rat brain. Histochemie 9, 126–135 (1967). https://doi.org/10.1007/BF00305856

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  • DOI: https://doi.org/10.1007/BF00305856

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