Summary
Recent immunohistochemical studies have shown that basal cells in human prostatic epithelium are not myoepithelial cells. Since in the literature the Dunning tumor, originally described as a rat prostate carcinoma derived from the dorsolateral prostate of a Copenhagen rat, was reported to have myoepithelial cells, a comparative immunohistochemical and ultrastructural study was performed in the H-, HIF- and AT3-lines of the Dunning tumor, the male accessory sex glands (ventral, dorsal, lateral prostate, coagulating gland, bulbourethral gland) and the mammary gland of both Copenhagen and Wistar rats. Mono- and polyclonal antibodies directed against intermediate filament proteins (cytokeratin, desmin, vimentin) and the contractile proteins (α-actin, muscle type specific myosin, tropomyosin) were used along with phalloidin decoration of F-actin. As in the human prostate, none of the rat prostate lobes in either strain did contain basal cells expressing cytokeratin along with α-actin, myosin and tropomyosin. Cells representing fully differentiated myoepithelial cells, however, were present as anticipated in the mammary gland, the bulbourethral gland and the H-tumor line of the Dunning tumor. This finding is difficult to reconcile with the contention of a prostatic origin of the H-Dunning tumor. Further studies are required to classify the epithelial parental tissue in order to define the true origin of the H-Dunning tumor and the tumor lines derived thereof.
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References
Achtstätter Th, Moll R, Moore B, Franke WW (1985) Cytokeratin polypeptide patterns of different epithelial of the human male urogenital tract: Immunofluorescence and gel electrophoretic studies. J Histochem Cytochem 33:415–426
Altmannsberger M, Dirk T, Osborn M, Weber K (1986) Immunohistochemistry of cytoskeletal filaments in the diagnosis of soft tissue tumors. Sem Diagn Pathol 3:306–316
Archer FL, Beck JS, Melvin JMO (1971) Localization of smooth muscle protein in myoepithelium by immunofluorescence. Am J Pathol 63:109–115
Aumüller G (1979) Prostate gland and seminal vesicles. In: Oksche A, Vollrath L (eds) Handbuch der mikroskopischen Anatomie des Menschen, vol VII/6. Springer, Berlin Heidelberg New York, pp 1–129
Aumüller G (1984) Morphologic and regulatory aspects of prostatic function. Anat. Embryol. 179:519–531
Aumüller G, Adler G (1979) Experimental studies of apocrine secretion in the dorsal prostate epithelium of the rat. Cell Tissue Res 198:145–158
Aumüller G, Seitz J (1990) Protein secretion and secretory processes in male accessory sex glands. Int Rev Cytol 121:127–231
Aumüller G, Seitz J, Heyns W, Flickinger CJ (1982) Intracellular localization of prostatic binding protein (PBP) in rat prostate by light and electron microscopic immunocytochemistry. Histochemistry 76:497–516
Aumüller G, Habenicht U-F, El-Etreby MF (1987a) Pharmacologically induced ultrastructural and immunohistochemical changes in the prostate of the castrated dog. Prostate 11:211–218
Aumüller G, Enderle-Schmitt U, Seitz J, Müntzing J, Chandler JA (1987b) Ultrastructure and immunohistochemistry of the lateral prostate in aged rats. Prostate 10:245–256
Aumüller G, Harley-Asp B, Seitz J (1989a) Differential reaction of secretory and non secretory proteins in hormone-treated Dunning tumor. Prostate 15:81–94
Aumüller G, Hüntemann S, Larsch K-P, Seitz J (1989b) Seminal proteins binding to spermatozoa. In: Motta P (ed) Developments in ultrastructure of reproduction. Alan R Liss New York, pp 241–248
Barwick KW, Mardi JA (1983) An immunohistochemical study of the myoepithelial cell in prostatic hyperplasia and neoplasia. Lab Invest 48:7A
Beckman Jr WC, Camps Jr JL, Weissman RM, Kaufman SL, Sanofsky SJ, Reddick RL, Siegal GP (1987) The epithelial origin of a stromal cell population in adenocarcinoma of the rat prostate. Am J Pathol 128:555–565
Brawer MK, Peehl DM, Stansey TA, Bostwick DG (1985) Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res 35:3663–3667
Drenckhahn D, Gröschel-Stewart U (1980) Localization of myosin, actin and tropomyosin in rat intestinal epithelium: immunohistochemical studies at the light and electron microscopic levels. J Cell Biol 86:475–482
Drenckhahn D, Gröschel-Stewart U, Unsicker K (1977) Immunofluorescence-microscopic demonstration of myosin and actin in salivary glands and exocrine pancreas of rat. Cell Tissue Res 183:273–279
Dunning WF (1963) Prostate cancer in the rat. Monogr Natl Cancer Inst 12:351–369
Evans GS, Chandler JA (1987) Cell proliferation studies in rat prostate: I. The proliferative role of basal and secretory epithelial cells during normal growth. Prostate 10:163–178
Feitz WFJ, Debruyne FMJ, Vooijs GP, Herman CJ, Ramaekers FCS (1986) Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues. J Urol 136:922–932
Gröschel-Stewart U, Magel E, Paul E, Neidlinger A-C (1989) Pig brain homogenates contain smooth muscle myosin and cytoplasmic myosin isoforms. Cell Tissue Res 257:137–139
Gugliotta P, Sapino A, Macri L, Skalli O, Gabbiani G, Bussolati G (1988) Specific demonstration of myoepithelial cells by anti-alpha smooth muscle actin antibody. J Histochem Cytochem 36:659–663
Heatfield BM (1987) In vitro model: Organ explant culture of normal and neoplastic human prostate. Prog Clin Biol Res 239:391–475
Isaacs JT, Isaacs WB, Feitz WFJ, Scheres J (1986) Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9:261–281
Ito S, Karnovsky MJ (1968) Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J Cell Biol 39:168A
Leong AS-Y, Gilham P, Milios J (1988) Cytokeratin and vimentin intermediate filament proteins in benign and neoplastic prostatic epithelium. Histopathology 13:435–442
Mao P, Angrist A (1966) The fine structure of the basal cell of human prostate. Lab Invest 15:1768–1782
Merchant DJ, Clarke SM, Ives K, Harris S (1983) Primary explant culture: An in vitro model of human prostate. Prostate 4:523–542
Nagle RB, Böcker W, Davis JR, Heid HW, Kaufmann M, Lucas DO, Jarasch E-D (1986) Characterization of breast carcinomas by two monoclonal antibodies distinguishing myoepithelial from luminal epithelial cells. J Histochem Cytochem 34:869–881
Nagle RB, Ahmann FR, McDaniel KM, Paquin ML, Clark VA, Celniker (1987) Cytokeratin characterization of human prostatic carcinoma and its derived cell lines. Cancer Res 47:281–286
Purnell DM, Heatfield BM, Anthony RL, Trump BF (1987) Immunohistochemistry of the cytoskeleton of human epithelium. Evidence for disturbed organization in neoplasia. Am J Pathol 126:384–395
Ramaekers FCS, Verhagen APM, Isaacs JT, Feitz WFJ, Moesker O, Schaart G, Schalken JA, Vooijs GP (1989) Intermediate filament expression and the progression of prostatic cancer as studied in the Dunning R-3327 rat prostatic carcinoma system. Prostate 14:323–339
Riva A, Usai E, Scarpa R, Cossu M, Lantini MS (1989) Fine structure of the accessory glands of the human male genital tract. In: Motta P (ed) Developments in ultrastructure of reproduction. Alan R Liss, New York, pp 233–240
Rowlatt C, Franks LM (1964) Myoepithelium in mouse prostate. Nature 202:707–708
Sinowatz F, Gabius H-J, Hellmann KP, Amselgruber W, Schneider MR (1990) Expression of endogenous receptors for neoglycoproteins in Dunning R3327 rat prostatic carcinoma. Prostate 16:173–184
Smolev JK, Coffey DS, Scott WW (1977) Experimental models for the study of prostatic adenocarcinoma. J Urol 118:216–220
Srigley JR, Darchick I, Hartwick RWJ, Klotz L (1990) Basal epithelial cells of human prostate gland are not myoepithelial cells. A comparative immunohistochemical and ultrastructural study with the human salivary gland. Am J Pathol 136:957–966
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunohistochemistry. J Histochem Cytochem 18:315–333
Stuewer D, Gröschel-Stewart U (1990) Immune response of rabbits to native G-actins. Biochim Biophys Acta 1039:5–11
Verhagen APM, Aalders TW, Ramaekers FCS, Debruyne FM, Schalken JA (1988) Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration. Prostate 13:25–38
Wernert N, Seitz G, Achtstätter Th (1987) Immunohistochemical investigation of different cytokeratins and vimentin in the prostate from the fetal period up to adulthood and in prostate carcinoma. Pathol Res Pract 182:617–626
Wilson EM, Viskochil DH, Bartlett RJ, Lea OA, Noyes CM, Petrusz P, Stafford DW, French FS (1981) Model systems for studies on androgen-dependent gene expression in the rat prostate. In: Murphy GP, Sandberg AA, Karr JP (eds) The prostatic cell: structure and function, part A. Alan R, Liss, New York, pp 351–380
Wulf E, Debobon A, Bantz FA, Faulstich H, Wieland T (1979) Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proc Natl Acad Sci USA 76:4498–4502
Zachary JM, Cleveland G, Kwock L, Lawrence T, Weissman RM, Nabell L, Fried FA, Staab EV, Risinger MA, Lin S (1986) Actin filament organization of the Dunning R3327 rat prostatic adenocarcinoma system: correlation with metastatic potential. Cancer Res 46:926–932
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Aumüller, G., Gröschel-Stewart, U., Altmannsberger, M. et al. Basal cells of H-Dunning tumor are myoepithelial cells. Histochemistry 95, 341–349 (1991). https://doi.org/10.1007/BF00266961
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DOI: https://doi.org/10.1007/BF00266961