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Identification of muramyl derivatives in mollusca gastropoda tissue

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Summary

Previous findings have demonstrated the presence of muramic acid and the lack of sialic acid in gastropod glycoconjugates from different tissues. The present study investigated the composition of muramyl derivatives in Mollusca Gastropoda tissue from the foot, mantle and periesophageal ganglia, using HRP-labeled lectins (LTA, UEA I, GSA IB4, GSA II, DBA, SBA, RCA II, WGA, PNA, ConA) and glycosidase digestion (neuraminidase, lysozyme, α-l-fucosidase, β-N-acetylglucosaminidase, α-N-acetylgalactosaminidase). Muramyl derivatives from the tissue examined showed some differences related to the composition of the terminal disaccharides. Indeed, foot and mantle mucocytes exhibited muramic acid in a terminal position, linked to (subterminal) N-acetylgalactosamine, whereas in neuron cells muramic acid was present in an internal position and linked to N-acetylglucosamine. Diversities also occurred between foot and mantle mucocytes with respect to the receptor sugar for penultimate N-acetylgalactosamine.

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Authors and Affiliations

  1. Department of Molecular, Cellular and Animal Biology, University of Camerino, via A. Moro 10, I-62032, Camerino (MC), Italy

    G. Menghi & D. Accili

  2. Department of Animal Biology, University of Modena, via Berengario 14, I-41100, Modena, Italy

    E. Ottaviani & A. M. Bolognani Fantin

Authors
  1. G. Menghi
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  2. E. Ottaviani
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  3. D. Accili
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  4. A. M. Bolognani Fantin
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Menghi, G., Ottaviani, E., Accili, D. et al. Identification of muramyl derivatives in mollusca gastropoda tissue. Histochemistry 96, 209–213 (1991). https://doi.org/10.1007/BF00271539

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  • DOI: https://doi.org/10.1007/BF00271539

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