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Quantification of carbonic anhydrase III and myoglobin in different fiber types of human psoas muscle

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Summary

Carbonic anhydrase (CA III) and myoglobin contents from isolated human muscle fibers were quantified using a sensitive time-resolved fluoroimmunoassay. Human psoas muscle specimens were freeze-dried, and single fibers were dissected out and classified into type I, IIA and IIB by myosin ATPase staining. Fiber typing was further confirmed by SDS-PAGE. CA III and myoglobin were found in all fiber types. Type I fibers contained higher concentrations of CA III and myoglobin than type IIA and IIB fibers. The relative concentrations of CA III in type IIA and IIB fibers were respectively 24% and 10% of that in type I fibers. The relative concentrations of myoglobin in type IIA and IIB fibers were 60% and 28% of that in type I fibers. Anti-CA III immunoblotting results from fiber-specific pooled samples agreed well with quantitative measurements. The results indicate that CA III is a more specific marker than myoglobin for type I fibers.

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Authors and Affiliations

  1. Department of Anatomy, University of Oulu, Kajaanintie 52 A, SF-90220, Oulu, Finland

    A. Zheng & H. K. Väänänen

  2. Rehabilitation and Physical Exercise Center, SF-41350, Laukaa as, Finland

    P. Rahkila

  3. Deaconess Institute of Oulu, SF-90100, Oulu, Finland

    J. Vuori, S. Rasi & T. Takala

Authors
  1. A. Zheng
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  2. P. Rahkila
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  3. J. Vuori
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  4. S. Rasi
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  5. T. Takala
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  6. H. K. Väänänen
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Zheng, A., Rahkila, P., Vuori, J. et al. Quantification of carbonic anhydrase III and myoglobin in different fiber types of human psoas muscle. Histochemistry 97, 77–81 (1992). https://doi.org/10.1007/BF00271284

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  • DOI: https://doi.org/10.1007/BF00271284

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