Summary
White blood cell DNA adducts were measured in coke workers, local controls and countryside controls using the 32P-postlabelling technique. The method detected aromatic adducts including those formed by polycyclic aromatic hydrocarbons (PAHs). Coke workers are heavily exposed to PAHs particularly when working at the batteries. A difference in adduct levels was noted between the coke workers at the battery as compared to other jobs. The adduct levels in the non-battery were higher than those in the countryside controls.
Similar content being viewed by others
References
Everson RB, Randerath E, Santella RM, Cefalo RC, Avitts TA, Randerath K (1986) Detection of smoking-related covalent DNA adducts in human placenta. Science 231:54–57
Gupta RC, Reddy MV, Randerath K (1982) 32P-postlabelling analysis of non-radioactive aromatic carcinogen-DNA-adducts. Carcinogenesis 3:1081–1092
Harris CC, Vähäkangas K, Newman MJ, Trves GE, Shamsuddin A, Sinopoli N, Mann DL, Wright WE (1985) Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers. Proc Natl Acad Sci USA 82:6672–6676
Haugen A, Becher G, Benestad C, Vähäkangas K, Trives GE, Newman MJ, Harris CC (1986) Determination of polycyclic aromatic hydrocarbons in the urine, benzo[a]pyrene diol epoxide DNA adducts in lymphocyte DNA, and antibodies to the adducts in sera from coke oven workers exposed to measured amounts of polycyclic aromatic hydrocarbons in the work atmosphere. Cancer Res 46:4178–4183
Hemminki A, Grzybowska E, Chorazy M, Twardowska-Sancha K, Sroczynski JW, Putman KL, Randerath K, Phillips DH, Hewer A, Santella RM, Young TL, Perera FP (1990) DNA adducts in humans environmentally exposed to aromatic compounds in an industrialized area of Poland. Carcinogenesis (in press)
Hemminki K, Perera FP, Phillips DH, Randerath K, Reddy MV, Santella RM (1988) Aromatic DNA adducts in white blood cells of foundry workers. In: Bartsch H, Hemminki K, O'Neille IK (eds) Methods for detecting DNA damaging agents in humans: applications in cancer epidemiology and prevention. IARC Scientific Publications 89:190–195
IARC (1984) IARC monographs on the evaluation of carcinogenic risks to humans, vol 34. Polynuclear Aromatic Compounds, part 3, Lyon IARC (1987) IARC monographs on the evaluation of carcinogenic risks to humans, vol 1–42 [Suppl 7]. Overall evaluations of carcinogenicity: an updating of IARC monographs, Lyon
Motykiewicz G, Szeliga J, Cimander B, Chorazy M (1989) Seasonal variation in mutagenic activity of air pollutants at an industrial district of Silesia. Mutat Res 223:243–251
Perera FP, Hemminki K, Young TL, Santella RM, Brenner D, Kelly G (1988) Detection of polycyclic aromatic hydrocarbonDNA adducts in white blood cells of foundry workers. Cancer Res 48:2288–2291
Phillips DH, Hemminki K, Alhonen A, Hewer A, Grover PL (1988a) Monitoring occupational exposure to carcinogens: detection by 32P-postlabelling of aromatic DNA adducts in white blood cells from iron foundry workers. Mutat Res 204:531–541
Phillips DH, Hewer A, Martin CN, Garner RC, King MM (1988b) Correlation of DNA adducts levels in human lung with cigarette smoking. Nature 336:790–792
Randerath E, Miller RH, Mittal D, Avitts TA, Dunsford HA, Randerath K (1989) Covalent DNA damage in tissues of cigarette smokers as determined by 32P-postlabeling assay. J Natl Cancer Inst 81:341–347
Reddy MV, Randerath K (1986) Nuclease P1-mediated enhancement of sensitivity of 32P-postlabelling test for structually diverse DNA adducts. Carcinogenesis 7:1543–1551
Redmond CK (1983) Cancer mortality among coke oven workers. Environ Health Persp 52:67–73
Rockette HE, Redmond CK (1985) Selection, follow-up, and analysis in the coke oven study. Natl Cancer Inst Monogr 67:89–94
Schimberg R, Pfäffli P, Tossavainen A (1978) Profilanalyse von polycyclischen aromatischen Kohlenwasserstoffen in Eisengiessereien. Straub 38:273–276
Shamsuddin AKM, Sinopoli NT, Hemminki K, Boesch RR, Harris CC (1985) Detection of benzo[a]pyrene: DNA adducts in human white blood cells. Cancer Res 45:66–68
Weston A, Rowe ML, Manchester DK, Farmer PB, Mann DL, Harris CC (1989) Fluorescence and mass spectral evidence for the formation of benzo[a]pyrene anti-diol-epoxide-DNA and -hemoglobin adducts in humans. Carcinogenesis 10:251–257
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hemminki, K., Grzybowska, E., Chorazy, M. et al. Aromatic DNA adducts in white blood cells of coke workers. Int. Arch Occup Environ Heath 62, 467–470 (1990). https://doi.org/10.1007/BF00379065
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00379065