Summary
Fluctuations in the activity of phenylalanine ammonia-lyase (PAL) in tissues of buckwheat seedlings were monitored by an intact cell assay and compared with results obtained with the conventional in vitro assay. Good correlation between measurements with the different assays was found for PAL activity changes during 1) growth of seedlings in the dark, 2) illumination of seedlings, and 3) incubation of hypocotyl segments. When leaf disks of buckwheat, strawberry, or sunflower were allowed to float on either water or a sugar solution, PAL activities determined with the intact cell assay increased irrespective of the presence of sugar, while PAL activities determined in cell homogenates increased only in disks floating on a sugar solution. Possible explanations for these discrepancies are discussed.
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Abbreviations
- PAL:
-
phenylalanine ammonia-lyase (E.C.4.3.1.5)
References
Amrhein, N., Zenk, M.H.: Concomitant induction of phenylalanine ammonia-lyase and cinnamic acid-4-hydroxylase during illumination of excised buckwheat hypocotyls. Naturwissenschaften 57, 312 (1970a)
Amrhein, N., Zenk, M.H.: Aktivitäten der Phenylalanin-Ammonium-Lyase (PAL) und Akkumulation von phenylpropanoiden Verbindungen während der Keimung von Buchweizen (Fagopyrum esculentum Moench) im Dunkeln. Z. Pflanzenphysiol. 63, 384–388 (1970b)
Amrhein, N., Zenk, M.H.: Untersuchungen zur Rolle der Phenylalanin-Ammonium-Lyase (PAL) bei der Regulation der Flavonoid-Synthese im Buchweizen (Fagopyrum esculentum Moench). Z. Pflanzenphysiol. 64, 145–168 (1971)
Amrhein, N., Gerhardt, J., Gödeke, K.-H.: The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue. I. Parameters of the assay. Planta (Berl.) 131, 33–40 (1976)
Attridge, T.H., Smith, H.: Evidence for a pool of inactive phenylalanine ammonia-lyase in Cucumis sativus seedlings. Phytochemistry 12, 1569–1574 (1973)
Camm, E.L., Towers, G.H.N.: Phenylalanine ammonia lyase. Phytochemistry 12, 961–973 (1973)
Creasy, L.L.: The increase in phenylalanine ammonia-lyase activity in strawberry leaf disks and its correlation with flavonoid synthesis. Phytochemistry 7, 441–446 (1968)
Creasy, L.L., Zucker, M.: Phenylalanine ammonia-lyase and phenolic metabolism. In: Metabolism and regulation of secondary plant products. Rec. Adv. Phytochem. Vol. 8, pp. 1–19. Eds.: Runeckles, V.C., Conn, E.E., New York: Academic Press 1974
Creasy, L.L., Zucker, M., Wong, P.P.: Anomalous effects of cycloheximide on phenylalanine ammonia-lyase: role of synthesis and inactivation in leaf disks of Helianthus annuus. Phytochemistry 13, 2117–2124 (1974)
Engelsma, G.: Low-temperature effects on phenylalanine ammonia-lyase activity in gherkin seedlings. Planta (Berl.) 91, 246–254 (1970)
Hopkins, W.G., Orkwiszewski, A.J.: Inhibition of phenylalanine ammonia lyase and enhancement of Avena coleoptile segment elongation by fluorophenylalanine. Canad. J. Bot. 49, 129–135 (1971)
Scherf, H., Zenk, M.H.: Induction of anthocyanin and phenylalanine ammonia-lyase formation by a high energy light reaction and its control through the phytochrome system. Z. Pflanzenphysiol. 56, 203–206 (1967a)
Scherf, H., Zenk, M.H.: Der Einfluß des Lichtes auf die Flavonoidsynthese und die Enzyminduktion bei Fagopyrum esculentum Moench. Z. Pflanzenphysiol. 57, 401–418 (1967b)
Zucker, M.: Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiol. 40, 779–784 (1965)
Zucker, M.: Light and enzymes. Ann. Rev. Plant Physiol. 23, 133–156 (1972)
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Amrhein, N., Gödeke, KH. The estimation of phenylalanine ammonia-lyase (PAL)-activity in intact cells of higher plant tissue. Planta 131, 41–45 (1976). https://doi.org/10.1007/BF00387343
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DOI: https://doi.org/10.1007/BF00387343