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Sucrose biosynthesis in Dunaliella

II. Isolation and properties of sucrose phosphate synthetase

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Abstract

Sucrose phosphate synthetase (EC 2.4.1.14) is the key enzyme for sucrose synthesis in Dunaliella tertiolecta. It has been partially purified and characterized. The enzyme contains one binding site for uridine diphosphoglucose and two binding sites for fructose-6-phosphate; it is allosterically controlled by fructose-6-phosphate. Inorganic phosphate stimulates the enzymic activity, particularly in the presence of higher concentrations of fructose-6-phosphate. Sucrose phosphate synthetase is not halophilic or halotolerant. The temperature dependence of the enzymic activity cannot fully explain the observed increase in sucrose synthesis in Dunaliella by elevated temperature.

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Abbreviations

F-6-P:

fructose 6-phosphate

UDP:

uridine biphosphate

UDPG:

uridine biphosphoglucose

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Author notes

  1. W. Müller

    Present address: Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800, Freiburg-Zähringen, FRG

Authors and Affiliations

  1. Institut für Chemische Pflanzenphysiologie der Universität, Auf der Morgenstelle 1, D-7400, Tübingen, Federal Republic of Germany

    W. Müller & K. Wegmann

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  1. W. Müller
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  2. K. Wegmann
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Müller, W., Wegmann, K. Sucrose biosynthesis in Dunaliella . Planta 141, 159–163 (1978). https://doi.org/10.1007/BF00387883

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