Abstract
Mitochondria from Vicia faba L. exhibit two pathways for the oxidation of endogenous NADH. One pathway is rotenone-sensitive and includes three phosphorylation sites, the other one is rotenone-insensitive and by-passes site I. This by-pass is located in the area of flavoproteins, but several lines of evidence suggest that the rotenone-insensitive oxidation of matrix-NADH is not mediated by the external NADH dehydrogenase. With saturating substrate concentrations the two pathways are superimposed in state 3 while in state 4 the electrons are transferred only via the by-pass. The rotenone-resistant electron flux is obviously regulated from the substrate side. We suggest that the function of the by-pass is to regulate the NADH/NAD+ ratio of the matrix space.
Similar content being viewed by others
Abbreviations
- BSA:
-
bovine serum albumin
- CCCP:
-
carbonylcyanide-m-chlorophenylhydrazone
- EDTA:
-
ethylenediaminetetraacetic acid
- HEPES:
-
N-2-hydroxy-ethylpiperazine-ethanesulphonic acid
- PVP:
-
polyvinylpyrrolidone
- TPP:
-
thiaminepyrophosphate
References
Bowman, E.J., Ikuma, H., Stein, H.J.: Citric acid cycle activity in mitochondria isolated from mung bean hypocotyls. Plant Physiol. 58, 426–432 (1976)
Brunton, C.J., Palmer, J.M.: Pathways for the oxidation of malate and reduced pyridine nucleotide by wheat mitochondria. Eur. J. Biochem. 39, 238–291 (1973)
Chance, B., Williams, G.R.: The respiratory chain and oxidative phosphorylation. Adv. Enzymol. 17, 65–134 (1956)
Coleman, O.D., Palmer, J.M.: The oxidation of malate by isolated plant mitochondria. Eur. J. Biochem. 26, 499–509 (1972)
Cunningham, W.P.: Oxidation of externally added NADH by isolated corn root mitochondria. Plant Physiol. 39, 699–703 (1964)
Day, D.A., Wiskich, J.T.: The oxidation of malate and exogenous reduced nicotinamide adenine dinucleotide by isolated plant mitochondria. Plant Physiol. 53, 104–109 (1974a)
Day, D.A., Wiskich, J.T.: The effect of exogenous nicotinamide adenine dinucleotide on the oxidation of nicotinamide adenine dinucleotide-linked substrates by isolated plant mitochondria. Plant Physiol. 54, 360–363 (1974b)
Day, D.A., Wiskich, J.T.: Isolation and properties of the outer membrane of plant mitochondria. Arch. Biochem. Biophys. 171, 117–123 (1975)
Douce, R., Christensen, E.L., Bonner, W.D.: Preparation of intact plant mitochondria. Biochim. Biophys. Acta 275, 148–160 (1972)
Douce, R., Manella, C.A., Bonner, W.D.: The external NADH dehydrogenases of plant mitochondria. Biochim. Biophys. Acta 292, 105–116 (1973)
Erecinska, M., Storey, B.T.: The respiratory chain of plant mitochondria. VII. Kinetics of flavoprotein oxidation in skunk cabbage mitochondria. Plant Physiol. 42, 618–624 (1970)
Ikuma, H., Bonner, W.D.: Properties of higher plant mitochondria III. Effects of respiratory inhibitors. Plant Physiol. 42, 1535–1541 (1967)
Jagow, G. von, Klingenberg, M.: Pathways of hydrogen in mitochondria from Saccharomyces carlsbergensis. Eur. J. Biochem. 12, 583–592 (1970)
Kkingenberg, M.: Localization of the glycerol-phosphatase dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur. J. Biochem. 13, 247–252 (1970)
Lamprecht, W., Trautschold, I.: In: Methoden der enzymatischen Analyse. Bergmeyer, H.U. ed., pp. 2151–2160. Weinheim: Verlag Chemie, 1974
Lee, C.P., Ernster, L.: In: The regulation of metabolic processes in mitochondria. Tager, J.M., Papa, S., Quagliariello, E., Slater, E.C. eds., pp. 218–222. Amsterdam: Elsevier, 1966
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Matlib, M.A., Kirkwood, R.C., Smith, J.E.: Oxidative and phosphorylative activities of tightly coupled mitochondria isolated from Vicia faba L. J. Exp. Bot. 22, 291–303 (1971)
Moreau, F., Lance, A.: Isolation and properties of external and internal membranes from plant mitochondria. Biochimie 54, 1227–1380 (1972)
Palmer, J.M., Coleman, J.O.D.: Multiple pathways of NADH oxidation in the mitochondrion. Horiz. Biochem. Biophys. 1, 220–260 (1974)
Palmer, J.M., Arron, G.P.: The influence of exogenous nicotinamide adenine di nucleotide on the oxidation of malate by Jerusalem Artichoke mitochondria. J. Exp. Bot. 27, 418–430 (1976)
Pomeroy, M.K.: The effect of nucleotides and inhibitors on respiration in isolated wheat mitochondria. Plant Physiol. 55, 51–58 (1975)
Solomos, T.: Cyanide-resistant respiration in higher plants. Ann. Rev. Plant Physiol. 28, 279–297 (1977)
Storey, B.T.: The respiratory chain of plant mitochondria. VI. Flavoprotein components of the respiratory chain of mung bean mitochondria. Plant Physiol. 46, 13–20 (1970)
Storey, B.T.: The respiratory chain of plant mitochondria. X. Oxidation-reduction potentials of the flavoproteins of skunk cabbage mitochondria. Plant Physiol. 48, 493–497 (1971)
Wilson, R.H., Hanson, J.B.: The effect of respiratory inhibitors on NADH, succinate and malate oxidation in corn mitochondria. Plant Physiol. 44, 1335–1341 (1969)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marx, R., Brinkmann, K. Characteristics of rotenone-insensitive oxidation of matrix-NADH by broad bean mitochondria. Planta 142, 83–90 (1978). https://doi.org/10.1007/BF00385124
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00385124