Summary
When cell suspension of soybean was cultured in the presence of colchicine (1.0x10-4 to 1.0x10-3 M), the degree of cell separation was greatly increased. This promotion of cell separation was accompanied with swelling of cells and modification of cell form. The treated suspension consisted predominantly of single cells and a few small aggregates, and could be repeatedly subcultured in the presence of 1.0x10-4 M colchicine, but could not at 1.0x10-3 M.
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References
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Umetsu, N., Ojima, K. & Matsuda, K. Enhancement of cell separation by colchicine in cell suspension cultures of soybean. Planta 125, 197–200 (1975). https://doi.org/10.1007/BF00388705
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DOI: https://doi.org/10.1007/BF00388705