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Cell organelles from crassulacean acid metabolism (CAM) plants

II. Compartmentation of enzymes of the crassulacean acid metabolism

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Abstract

The intracellular distribution of enzymes involved in the Crassulacean acid metabolism (CAM) has been studied in Bryophyllum calycinum Salisb. and Crassula lycopodioides Lam. After separation of cell organelles by isopycnic centrifugation, enzymes of the Crassulacean acid metabolism were found in the following cell fractions: Phosphoenolpyruvate carboxylase in the chloroplasts; NAD-dependent malate dehydrogenase in the mitochondria and in the supernatant; NADP-dependent malate dehydrogenase and phosphoenolpyruvate carboxykinase in the chloroplasts; NADP-dependent malic enzyme in the supernatant and to a minor extent in the chloroplasts; NAD-dependent malic enzyme in the supernatant and to some degree in the mitochondria; and pyruvate; orthophosphate dikinase in the chloroplasts. The activity of the NAD-dependent malate dehydrogenase was due to three isoenzymes separated by (NH4)2SO4 gradient solubilization. These isoenzymes represented 17, 78, and 5% of the activity recovered, respectively, in the order of elution. The isoenzyme eluting first was associated with the mitochondria and the second isoenzyme was of cytosolic origin, while the intracellular location of the third isoenzyme was probably the peroxisome. Based on these findings, the metabolic path of Crassulacean acid metabolism within cells of CAM plants is discussed.

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Author notes

  1. M. Herbert

    Present address: Lehrstuhl für Medizinische Mikrobiologie, Rubruniversität Bochum, Postfach 102148, D-4630, Bochum 1, Federal Republic of Germany

Authors and Affiliations

  1. Fachbereich Biologie, Universität Kaiserslautern, Pfaffenbergstraße, D-6750, Kaiserslautern, Federal Republic of Germany

    C. Schnarrenberger, D. Groß, Ch. Burkhard & M. Herbert

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  1. C. Schnarrenberger
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  2. D. Groß
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  3. Ch. Burkhard
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  4. M. Herbert
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New address: Institut für Pflanzenphysiologie und Zellbiologie, Freie Universität Berlin, Königin-Luise-Straße 12-16a. D-1000 Berlin 33

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Schnarrenberger, C., Groß, D., Burkhard, C. et al. Cell organelles from crassulacean acid metabolism (CAM) plants. Planta 147, 477–484 (1980). https://doi.org/10.1007/BF00380191

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