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Use of an (n, α) nuclear reaction to study the long-distance transport of boron in trifolium repens after foliar application

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Abstract

The growth of white clover (Trifolium repens L.) is severely inhibited by boron starvation, but a foliar treatment with boric acid can transitorily alleviate the deficiency symptoms. The 10B(n ,α)7 Li nuclear reaction has been used to study boron transport in the plant after foliar application. More than 98% of the boron supplied remained at the point where it was applied to the leaves, and less than 2% was useful to the growth of the treated plant. This small “efficient” portion of boron was quite mobile. It was distributed to the different parts of the plant, then was transferred from the oldest parts to the newly formed leaves. Physiological and agronomical implications of these data are discussed.

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References

  • Agulhon, H. (1910) Emploi du bore comme engrais catalytique. C.R. Acad. Sci. Paris 150, 288–290

    Google Scholar 

  • Askew, H.O., Chittenden, E. (1936) The use of borax in the control of “internal cork” of apples. III Effect of borax sprays on the boron status of fruit and incidence of “internal cork” in apples. J. Pomol Hortic. Sci. 14, 242–245

    Google Scholar 

  • Bertrand, G., Silberstein, L. (1939) La teneur en bore des feuilles dans la maladie du coeur de la betterave et d'autres plantes. C.R. Acad. Sci. Paris 209, 270–271

    Google Scholar 

  • Betrand, G., de Waal, H. (1936) Sur la teneur comparative en bore dans les diverses parties de la graine. C.R. Acad. Sci. Paris 202, 605–609

    Google Scholar 

  • Boeseken, J. (1949) Use of boric acid for the determination of the configuration of carbohydrates. Adv. Carbohydr. Chem. 4, 189–210

    Google Scholar 

  • Bowen, J.E. (1972) Effect of environmental factors on water utilization and boron accumulation and translocation in sugarcane. Plant Cell Physiol. 13, 703–714

    Google Scholar 

  • Bowen, J.E., Nissen, P. (1976) Boron uptake by excised barley roots. I. Uptake into the free space. Plant Physiol. 57, 353–357

    Google Scholar 

  • Bowen, J.E., Nissen, P. (1977) Boron uptake by excised barley roots. II. Characteristics and kinetics of active uptake. Physiol. Plant. 41, 109–115

    Google Scholar 

  • Carpenter, B.S., Cheek, C.H. (1970) Trace determination of uranium in biological material by fission track counting. Anal. Chem. 42, 121–123

    Google Scholar 

  • Chapman, K.S.R., Jackson, J.F. (1974) Increased RNA alelling in boron deficient root-tip segments. Phytochemistry 13, 1311–1318

    Google Scholar 

  • Dible, W.T., Truog, E., Berger, K.C. (1954) Boron determination in soils and plants. Simplified curcumin procedure. Anal. Chem. 26, 418–421

    Google Scholar 

  • Duval, Y., Thellier, M., Heurteaux, C., Wissocq, J.C. (1980) Detection of stable isotopes with a (n, α) nuclear reaction: application to the measurement of unidirectional fluxes of borate in a plant. J Radioanal. Chem. 55, 297–306

    Google Scholar 

  • Fleischer, R.L., Price, P.B. (1963) Tracks of charged particles in high polymers. Science 140, 1221–1222

    Google Scholar 

  • Garin, A., Thellier, M. (1958) Méthode de microdétermination et de microdosage du bore par activation, aux neutrons et examen microscopique des autoradiographies. Bull. Micr. Appl. 8, 129–148

    Google Scholar 

  • Gerrard, W. (1961) Organic chemistry of boron. Academic Press, London, p 10–21

    Google Scholar 

  • Hewitt, E.J. (1952) Sand and water culture methods used in the study of plant nutrition. Commonwealth agricultural Bureaux, East Malling, Maidstone, Kent

    Google Scholar 

  • Homes, M., Ansiaux, J.R., Van Schoor, G. (1953) L'aquiculture. 2nd edéd. Ministère des Colonies de Belgique, Direction de l'Agriculture, Bruxelles

    Google Scholar 

  • Isbell, H.S., Brewster, J.F., Holt, N.B., Frush, H.L. (1948) Behaviour of certain sugars and sugar alcohols in presence of tetraborates, correlation of optical rotation and compound formation. U.S. Natl. Bur. Standards. Res. Paper 40, 129–149

    Google Scholar 

  • Kibalenko, A.P., Sidorshina, T.N., Poedinok, N.T., Kharchenko, N.A., Demchenko, T.I. (1977) Role of boron in increasing sugar content and technological qualities of sugar beet. Dopavidi Akademiji narsk Ukrajins koji, R.S.R. 3, 249–251

    Google Scholar 

  • Kouchi, H. (1977) Rapid cessation of mitosis and elongation of root tip cells of Vicia faba as affected by boron deficiency. Soil Sci. Plant Nutr. 23, 1, 113–115

    Google Scholar 

  • Martini, F., Devauchelle, G., Thellier, M. (1978) Etude cinétique de l'absorption foliaire de borate. Physiol. Vég. 16, 255–264

    Google Scholar 

  • Martini, F., Stelz, T., Duval, Y., Hartmann, A., Wissocq, J.C., Thellier, M. (1977) In: Echanges ioniques transmembranaires chez les végétaux, pp. 511–520, Thellier, M., Monnier, A., Demarty, M., Dainty, J., eds. CNRS, Paris and Université, Rouen

    Google Scholar 

  • Martini, F., Thellier, M. (1975) Study, with help of the 33 B( 10 n, 24 α) 37 Li nuclear reaction, on the distribution of boron in white clover after foliar application. Newsletter Applic. Nuclear Meth. Biol. Agric. 4, 26–29

    Google Scholar 

  • Maze, P. (1915) Détermination des éléments minéraux rares nécessaires au développement du maïs. C.R. Acad. Sci. Paris 160, 211–214

    Google Scholar 

  • Montgomery, F.H. (1951) The effect of boron on the growth and seed production of alsike clover. Can. J. Bot. 29, 597–605

    Google Scholar 

  • Robertson, G.A., Loughman, B.C. (1974) Response to boron deficiency: a comparison with responses produced by chemical methods of retarding root elongation. New Physiol. 73, 821–832

    Google Scholar 

  • Skok, J., MacIlrath, W.J. (1958) Distribution of boron in cells of dicotyledonous plants in relation to growth. Plant Physiol. 33, 428–431

    Google Scholar 

  • Smith, F.W. (1948) Some relationships of boron to the growth of legumes on southenstern Kansas soils. Soil Sci. Soc. Am. Proc. 13, 358–361

    Google Scholar 

  • Tanada, T. (1978) Boron-key element in the actions of phytochrome and gravity. Planta 143, 109–111

    Google Scholar 

  • Thellier, M. (1963) Contribution à l'étude de la nutrition en bore des végétaux. Thèse de Doctorat, Paris

  • Thellier, M., Duval, Y., Demarty, M. (1979) Borate exchanges of Lemma minor L. as studied with the help of the enriched stable isotopes and of a (n, α) nuclear reaction. Plant Physiol. 63, 283–288

    Google Scholar 

  • Thellier, M., le Guiel, J. (1967) Etude, grâce à l'isotope stable 10B, de l'absorption du borate par la Lemna minor L. C.R. Acad. Sc. Paris 264, 292–295

    Google Scholar 

  • Thellier, M., Stelz, T., Wissocq, J.C. (1976) Radioautography using homogeneous detectors. J. Microsc. Biol. Cell. 27, 157–168

    Google Scholar 

  • Van de Venter, H.A., Currier, H.B. (1977) The effect of boron deficiency on callose formation and 14C translocation in bean (Phaseolus vulgaris L.) and cotton (Gossypium hirsutum L.). Am. J. Bot. 64, 7, 861–865

    Google Scholar 

  • Wallace, A., Bear, F.E. (1949) Influence of potassium and boron on nutrient element balance and growth of ranger alfalfa. Plant Physiol. 24, 664–680

    Google Scholar 

  • Whittington, W.J. (1957) The role of boron in plant growth. I the effect on general growth, seed production, and cytological behaviour. J. Exp. Bot. 8, 353–367

    Google Scholar 

  • Zittle, C.A. (1951) Reaction of borate with substances of biological interest. Adv. Enzymol. 12, 493–499

    Google Scholar 

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Martini, F., Thellier, M. Use of an (n, α) nuclear reaction to study the long-distance transport of boron in trifolium repens after foliar application. Planta 150, 197–205 (1980). https://doi.org/10.1007/BF00390826

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