Abstract
Cell wall and thus cellulose microfibril formation in the presence of Congo red or Calcofluor white by Oocystis solitaria autospores was investigated ultrastructurally and chemically. The prevention of microfibril formation by both substances is accompanied by drastic changes of microfibril synthesis and orientation as well as the morphology of plasma-membrane-associated E-face terminal complexes. Removal of Congo red and Calcofluor white from the culture medium results in the recovery of microfibril formation, of a normal patterned microfibril arrangement, and of terminal complexes with extending microfibril imprints.
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Abbreviations
- CW:
-
Calcofluor white
- CR:
-
Congo red
- GB:
-
granule bands
- TC:
-
terminal complex
References
Arnon, D.J. (1949) Copper enzymes in isolated chloroplasts and polyphenoloxidases in Beta vulgaris. Plant Physiol. 24, 1–15
Benziman, M., Haigler, C.H., Brown, R.M., White, A., Cooper, K. (1980) Cellulose biogenesis; polymerization and crystallization are coupled processes in Acetobacter xylinum. Proc. Natl. Acad. Sci. USA 77, 6678–6682
Brown, R.M., Montezinos, D. (1976) Cellulose microfibrils: visualization of biosynthetic and orienting complexes in assocation with the plasma membrane. Proc. Natl. Acad. Sci. USA 73, 143–147
Carpita, N. (1982) Cellulose synthesis in detached cotton fibers. In: Cellulose and other natural polymer systems: biogenesis, structure, and degradation, pp. 225–242, Brown, R.M., ed. Plenum Press, New York
Galbraith, D.W. (1981) Microfluoremetric quantitation of cellulose biosynthesis by plant protoplasts using Calcofluor white. Physiol. Plant. 53, 111–116
Gooday, G.W. (1983) The microbial synthesis of cellulose, chitin, and chitosan. Progr. Ind. Microbiol. 18 (in press)
Haigler, C.H., Benziman, M. (1982) Biogenesis of cellulose I microfibrils occurs by cell-directed self-assembly in Acetobacter xylinum. In: Cellulose and other natural polymer systems: biogenesis, structure, and degradation, pp. 273–297, Brown, R.M., ed. Plenum Press, New York
Haigler, C.H., Brown, R.M., Benziman, M. (1980) Calcofluor white ST alters the in vivo assembly of cellulose microfibrils. Science 210, 903–906
Harrington, B.J., Raper, K.B. (1968) Use of fluorescent brightener to demonstrate cellulose in the cellular slime molds. Appl. Microbiol. 16, 106–113
Maeda, H., Ishida, M. (1967) Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener. J. Biochem. 62, 276–278
Quader, H. (1981a) Effects of deuterium oxide on microtubules and microfibrils in Oocystis solitaria. In: Cell walls '81, pp. 198–205, Robinson, D.G., Quader, H., eds. Wissenschaftliche Verlagsgesellschaft, Stuttgart
Quader, H. (1981b) Interruption of cellulose microfibril crystallization. Naturwissenschaften 67, 428
Quader, H. (1982) The microtubule-microfibril-syndrome in Oocystis solitaria: regulatory aspects. In: Microtubules in microorganisms, pp. 313–324, Cappuccinelli, P., Morris, H.R., eds. Marcel Dekker, New York
Quader, H., Robinson, D.G. (1981) Oocystis solitaria: a model organism for understanding the organization of celllulose synthesis. Ber. Dtsch. Bot. Ges. 94, 75–84
Quader, H., Wagenbreth, I., Robinson, D.G. (1978) Structure, synthesis and orientation of microfibrils. V. On the recovery of Oocystis solitaria from microtubule inhibitor treatment. Cytobiologie 18, 39–51
Roberts, E., Seagull, R.W., Haigler, C.H., Brown, R.M., Jr. (1982) Alteration of cellulose microfibril formation in eucaryotic cells: calcofluor White interferes with microfibril assembly and orientation in Oocystis apiculata. Protoplasma 113, 1–9
Robinson, D.G. (1981) The assembly of polysaccharide fibrils. In: Encyclopedia of plant physiology, N.S., vol. 13 B: Plant carbohydrates II, pp. 25–28, Tanner, W., Loewus, F.A., eds. Springer, Berlin Heidelberg New York
Robinson, D.G., Preston, R.D. (1972) Plasmalemma structure in relation to microfibril biosynthesis in Oocystis. Planta 104, 234–246
Robinson, D.G., Quader, H. (1980) Structure, synthesis and orientation of microfibrils. VII. Microtubule reassembly in vivo after cold treatment in Oocystis and its relevance to microfibril orientation. Eur. J. Cell Biol. 21, 229–230
Robinson, D.G., Quader, H. (1981a) Towards cellulose synthesis in vitro from plant extracts. J. Theor. Biol. 92, 483–495
Robinson, D.G., Quader, H. (1981b) Structure, synthesis, and orientation of microfibrils. IX. A freeze-fracture investigation of the Oocystis plasma membrane after inhibitor treatments. Eur. J. Cell Biol. 25, 278–288
Robinson, D.G., Quader, H. (1982) The microtubule-microfibril-syndrome. In: The cytoskeleton in plant growth and development, pp. 109–126, Lloyd, C., ed. Academic Press, London
Roe, J.A. (1955) The determination of sugar in blood and spinal fluid with the anthrone reagent. J. Biol. Chem. 212, 335–343
Updegraff, D.M. (1969) Semimicro determination of cellulose in biological materials. Anal. Biochem. 32, 420–424
Willison, J.H.M. (1983) The cellulose microfibril: its structure, assembly, and organization in cell walls. Int. Rev. Cytol. (in press)
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Quader, H., Robinson, D.G. & van Kempen, R. Cell wall development in Oocystis solitaria in the presence of polysaccharide binding dyes. Planta 157, 317–323 (1983). https://doi.org/10.1007/BF00397402
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DOI: https://doi.org/10.1007/BF00397402