Abstract
The in vitro activity of polysomal polyadenylated RNA (poly(A)RNA) was studied using chick-pea (Cicer arietinum L.) embryonic axes subjected to treatments retarding germination (H2O 30°C and abscisic acid [ABA] 30°C) or inducing a false germination (thiourea 30°C) in which normal protein synthesis and growth did not occur. All treatments induced a smaller proportion of poly(A)RNA compared with the control (H2O 25°C). However, poly(A)RNA obtained in the presence of ABA had a similar in vitro activity to that of the control. The translation of mRNA from embryonic axes germinated at high temperatures was extensively blocked (70%) by methyl-7-guanosine-5′-triphosphate, whereas mRNA translation from axes treated with H2O-25°C and ABA was completely blocked (100%), indicating a greater cap dependence in the latter cases. Polyacrylamide gel electrophoresis showed that ABA and H2O-30°C each induced the synthesis of a polypeptide with an approximate Mr of 32 kDa, probably a germination regulator. It is suggested that ABA and high temperatures could regulate germination at the translational level as well as affecting ionic-exchange properties, as has been previously demonstrated (Hernández-Nistal et al. 1983, Physiol. Plant. 57, 273–278).
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Abbreviations
- ABA:
-
abscisic acid
- Poly (A) RNA:
-
polyadenylated RNA
- TU:
-
thiourea
References
Aldasoro, J.J., Matilla, A., Nicolás, G. (1981) Effects of ABA fusicoccin and thiourea on germination and K+ and glucose uptake in chick-pea seeds at different temperatures. Physiol. Plant. 53, 139–145
Aviv, II., Leder, P. (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymydylic acid-cellulose. Proc. Natl. Acad. Sci. USA 69, 1408–1412
Bewley, J.D. (1982) Protein and nucleic acid synthesis during seed germination and early seedling growth. In: Encyclopedia of plant physiology, vol 14A: Nucleic acid and proteins in plants I, pp. 559–586, Boulter, D. Parthier, B., eds. Springer, Berlin Heidelberg New York
Blowers, L.F., Stormonth, D., Bray, C. (1980) Nucleic acid and protein synthesis and loss of vigour in germinating wheat embryos. Planta 150, 19–25
Caers, L.I., Peumans, W.J., Carlier, A.R. (1979) Preformed and newly synthesized messenger RNA in germinating wheat embryos. Planta 144, 491–496
Datta, K., Marsh, L., Marcus, A. (1983) Early growth of wheat embryonic axes and the synthesis of RNA and DNA. Plant Physiol. 72, 394–397
Dommes, J., Van de Walle, C. (1983) Newly synthesized mRNA is translated during the initial imbibition phase of germinating maize embryos. Plant Physiol. 73, 484–487
Dure, L.S., III, Greenway, S.C., Galau, G.A. (1981) The developmental biochemistry of cotton seed embryogenesis and germination XIV. Changes in mRNA populations as shown by in vivo and in vitro protein synthesis. Biochemistry 20, 4162–4168
Dure, L.S., III, Capdevilla, A.M., Greenway, S.C. (1980) Messenger RNA domains in the embryogenesis of cotton cotyledons. In: Genome organization and expression in plants, pp. 124–146, Leaver, C.J., ed. Plenum Publishing Corp., New York
Galli, M.G., Miracca, P., Sparvoli, E. (1979) Interaction between abscisic acid and fusicoccin during germination and postgerminative growth in Haplopappus gracilis. Plant Sci. Lett. 14, 105–111
Hernández-Nistal, J., Aldasoro, J.J., Rodriguez, D., Matilla, A., Nicolás, G. (1983) Effect of thiourea on the ionic content and dark fixation of CO2 in embryonic axes of Cicer arictinum L. Physiol. Plant. 57, 273–278
Ho, T.-h.D. (1979) On the mode of action of abscisic acid in barley aleurone cells. (Abstr.) Plant Physiol. 63, Suppl., 79
Karssen, C.M., Brinkhorst-van der Swan, D.L.C., Breekland, A.E., Koorneef, M. (1983) Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thalianc (L.) Heynh. Planta 157, 158–165
Kozak, M. (1980) Influence of mRNA secondary structure on binding and migration of 40 S ribosomal subunits. Cell 19, 79–80
Kozak, M., Shatkin, A.J. (1978) Identification of features in 5′ terminal fragments from reovirus mRNA which are important for ribosome binding. Cell 13, 201–212
Laemmli, U.K. (1970) Cleavage of structure proteins during the assembly of the head bacteriophage T4. Nature 227, 680–685
Marrè, E. (1970) Fusicoccin: a tool in plant physiology. Annu. Rev. Plant Physiol. 30, 273–288
Matilla, A., Nicolás, G., Sierra, J.M. (1982) Changes in messenger RNA in Cicer arietinum L. cotyledons seeds during germination. Plant Sci. Lett. 25, 209–217
Matilla, A., Nicolás, G., Vicente, O., Sierra, J.M. (1980) Preformed mRNA in cotyledons of ungerminated seeds of Cicer arietinum L. Plant Physiol. 65, 1128–1132
Payne, P.I. (1976) The long-lived messenger ribonucleic acid on flowering-plant seeds. Biol. Rev. 51, 329–363
Reynolds, T., Thompson, P.A. (1971) Characterization of the high temperature inhibition of germination of lettuce. Physiol. Plant 24, 544–547
Riley, G.T.P. (1981) Effects of high temperature on protein synthesis during germination of maize (Zea mays L.). Planta 151, 75–80
Roberts, B.E., Paterson, B.M. (1973) Efficient translation of tobacco mosaic virus RNA and rabbit globin 9 S RNA in a cell free system from commercial wheat germ. Proc. Natl. Acad. Sci. USA 70, 2330–2334
Rodriguez, D., Matilla, A., Hernández-Nistal, J., Aldasoro, J.J., Nicolás, G. (1983) Germination of Cicer arietinum L. and thiourea-induced phytotoxicity. Physiol. Plant. 57, 267–272
Rodriguez, D., Matilla, A., Nicolás, G. (1982) Reactivation of nucleic acids synthesis during early germination of Cicer arietinum L. embryonic axis. Acta Physiol. Plant. 4, 97–102
Smith, C.A., Bray, C.M. (1982) Intracellular levels of polyadenylated RNA and loss of vigour in germinating wheat embryos. Planta 156, 413–420
Spiegel, S., Marcus, A. (1975) Polyribosome formation in early wheat embryo germination independent of their transcription or polyadenylation. Nature 256, 228–230
Walbot, V., Clutter, K., Sussex, I. (1975) Effects of abscisic acid on growth, RNA metabolism and respiration in germinating bean axes. Plant Physiol. 56, 570–574
Whenham, R.J., Fraser, R.S.S. (1980) Stimulation by abscisic acid of RNA synthesis in discs from healthy and tobacco mosaic virus-infected tobacco leaves. Planta 150, 349–353
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Rodriguez, D., Nicolás, G., Aldasoro, J.J. et al. Altered development of polysomal RNA activity in chick-pea (Cicer arietinum L.) embryonic axes. Effects of abscisic acid and temperature. Planta 164, 517–523 (1985). https://doi.org/10.1007/BF00395969
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DOI: https://doi.org/10.1007/BF00395969