Abstract
The perisperm of seeds of Agrostemma githago contains starch reserves which constitute 40% of the dry weight of the mature seed. These starch reserves were mostly broken down between 48 and 96 h after initiation of imbibition. (Germination occurred after 24 h.) The mode of starch degradation showed close parallels with the breakdown of the starchy endosperm in cereals. Thus, between 24 and 96 h the cotyledons secreted α-amylase (EC 3.2.1.1) whereas other degradative enzymes in the perisperm, β-amylase (EC 3.2.1.2) and maltase (EC 3.2.1.20), appeared to originate in the perisperm itself. Cotyledons secreted similar levels of α-amylase in the presence and absence of exogenous starch, indicating that secretion is an internal developmental event of the embryo. By isoelectric focussing the secreted α-amylase was separated into two isoenzymes. In the cotyledons, several other starch-degrading isoenzymes were present but were not secreted.
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Abbreviations
- CHA:
-
cycloheptaamylose
- IEF:
-
isoelectric focussing
References
Bernhardt, D., Hecker, M., Bernhardt, H. (1978) Eignung intakter Caryophyllaceen-Samen für die Bestimmung der DNA-, RNA-und Proteinsynthesen durch Einbauversuche. Biochem. Physiol. Pflanz. 172, 263–270
Bewley, J.D., Black, M. (1978) Physiology and biochemistry of seeds in relation to germination, vol. I: Development, germination and growth. Springer, Berlin Heidelberg New York
Borriss, H., Arndt, M. (1956) Die Temperaturabhängigkeit der Stärkebildung in Agrostemma-Embryonen und ihre Beziehung zur Keimungsbereitschaft der Samen. Naturwissenschaften 43, 255–256
Borriss, H., Wiehle, V. (1974) Produktion und Sekretion von Amylase durch dormante und nachgereifte Agrostemma-Embryonen. Biol. Rundsch. 12, 197–200
Chrispeels, M.J., Varner, J.E. (1967) Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406
Davies, H.V., Slack, P.T. (1981) The control of food mobilization in seeds of dicotyledonous plants. New Phytol. 88, 41–51
de Klerk, G.J. (1981) Increase of protein synthesis after isolation in dormant and afterripened Agrostemma githago L. embryos. Planta 153, 524–529
de Klerk, G.J. (1983) Protein synthesis in ripening, dormant and afterripened Agrostemma githago L. seeds. Ph.D. thesis, University of Nijmegen
de Klerk, G.J. (1984) The biosynthesis of storage proteins in ripening Agrostemma githago L. seeds. Plant Physiol. 74, 516–524
de Klerk, G.J., Smulders, R. (1984) Protein synthesis in embryos of dormant and germinating Agrostemma githago L. seeds. Plant Physiol. 75, 929–935
Earle, F.R., Jones, Q. (1962) Analyses of seed samples from 113 plant families. Econ. Bot. 16, 221–250
Halmer, P. (1985) The mobilization of storage carbohydrates in germinated seeds. Physiol. Vég. 23, 107–125
Halmer, P., Bewley, J.D. (1981) Control by external and internal factors over the mobilization of reserve carbohydrates in higher plants. In: Encyclopedia of plant physiology, N.S., vol. 13b: Plant carbohydrates II, pp. 748–793, Loewus, F.A., Tanner, W., eds. Springer, Berlin Heidelberg New York
Hecker, M., Bernhardt, D., Köhler, K.-H., Schmerder, B., Dunger, H., Knösche, R. (1979) Synthesis of DNA and of hydrolytic enzymes in cotyledons of germinating seeds of Agrostemma githago L. Biochem. Physiol. Pflanz. 174, 523–536
Hübel, M. (1966) Untersuchungen über die Beeinflussung der Nachreifung von Agrostemma-Samen durch Temperatum und Wassergehalt. Flora 157, 109–130
Ilan, I., Gepstein, S. (1981) Hormonal regulation of food reserve breakdown in germinating dicotyledonous seeds. Isr. J. Bot. 29, 193–206
Jacobsen, J.V., Scandalios, J.G., Varner, J.E. (1970) Multiple forms of amylase induced by gibberellic acid in isolated barley aleurone layers. Plant Physiol. 45, 367–371
Kok, H. (1983) Starch degradation in Agrostemma githago L. seeds. M.Sc. thesis, University of Nijmegen
Laidman, D.L. (1982) Control mechanisms in the mobilisation of stored nutrients in germinating cereals. In: The physiology and biochemistry of seed development, dormancy and germination, pp. 371–405, Khan, A.A., ed. Elsevier Biomedical Press, Amsterdam
Lehnhardt, L., Wiedmann, M., Günther, G. (1981) Activity and multiple forms of hydrolytic enzymes in germinating sugar beets (Beta vulgaris L.). Biochem. Physiol. Pflanz. 176, 638–658
MacGregor, A.W. (1983) Cereal α-amylase: Synthesis and action pattern. In: Seed proteins, pp. 1–34, Daussant, J., Mossé, J., Vaughan, J., eds. Academic Press, London New York
Martin, A.C. (1946) The comparative internal morphology of seeds. Am. Midl. Natural. 36, 513–560
Nitta, Y., Mizushima, M., Hiromi, K., Ono, S. (1971) Influence of molecular structures of substrates and analogues on Taka-amylase A catalyzed hydrolysis. I. Effect of chain length of linear substrates. J. Biochem. (Tokyo) 69, 567–576
Ranki, H., Sopanen, T. (1984) Secretion of α-amylase by the aleurone layer and the scutellum of germinating barley grain. Plant Physiol. 75, 710–715
Robyt, J., French, D. (1963) Action pattern and specifity of an amylase from Bacillus subtilis. Arch. Biochem. Biophys. 100, 451–467
Scandalios, J.G. (1969) Genetic control of multiple molecular forms of enzymes in plants: a review. Biochem. Genet. 3, 37–79
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Dedicated to Professor H.F. Linskens on the occasion of his 65th birthday
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de Klerk, G.J., Willekens, P., Kok, H. et al. Amylolytic activity in germinated Agrostemma githago L. seeds. Planta 168, 77–83 (1986). https://doi.org/10.1007/BF00407012
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DOI: https://doi.org/10.1007/BF00407012