Abstract
Growth, water content, osmotic pressure and solute content were examined for normal potato (Solanum tuberosum L. cv. Desiree) and a derivative (line D9X8a), which was genetically transformed with TL-DNA from Agrobacterium rhizogenes. Plants were grown (i) in vitro, (ii) in a growth chamber and (iii) in the field. In vitro, the transformed potato plants produced more biomass than the untransformed plants, partly because they had a higher water content. Potassium concentration and osmotic pressure were lower in cell sap extracted from the transformed potato shoots. In some cases the difference was as much as 50%. These differences were less clear, absent or reversed in plants from a growth chamber or from the field. In the field, however, transformed potato senesced early. It is suggested that a cellular basis for these observations may be changes induced by Ri TL-DNA expression products in plant membrane properties.
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Abbreviations
- Ri:
-
root inducing
- Ti:
-
tumour inducing
- T-DNA:
-
transferred DNA
References
Ackermann, C. (1977) Pflanzen aus Agrobacterium rhizogenes — Tumoren an Nicotiana tabacum. Plant Sci. Lett. 8, 23–30
Akiyoshi, D.E., Morris, R.O., Hinz, R., Mischke, B.S., Kosuge, T., Garfinkel, D.J., Gordon, M.P., Nester, E.E. (1983) Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA. Proc. Natl. Acad. Sci. USA 80, 407–411
Barry, G.F., Rogers, S.G., Fraley, R.T., Brand, L. (1984) Identification of a cloned cytokinin biosynthetic gene. Proc. Natl. Acad. Sci. USA. 81, 4776–4780
Chilton, M-D., Drummond, M.H., Merlo, D.J., Sciaky, D., Montoya, A.L., Gordon, M.P., Nester, E.W. (1977) Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11, 263–271
Garfinkel, D.J., Simpson, R.B., Ream, L.W., White, F.F., Gordon, M.P., Nester, E.W. (1981) Genetic analysis of crown gall: fine structure map of the T-DNA by site directed mutagenesis. Cell 27, 143–153
Huffman, G.A., White, F.F., Gordon, M.P., Nester, E.W. (1984) Hairy-root inducing plasmid: physical map and homology to tumor inducing plasmids. J. Bacteriol. 157, 269–276
Inze, D., Follin, A., Van Lysebettens, M., Simoens, C., Genetallo, C. Van Montagu, M., Schell, J. (1984) Genetic analysis of the individual T-DNA genes of Agrobacterium tumefaciens: further evidence that two genes are involved in indole-3-acetic-acid synthesis. Mol. Gen. Genet. 194, 265–274
Jouanin, L. (1984) Restriction map of an Agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid 12, 91–102
Leemans, J., Deblaere, R., Willmitzer, L., de Greve, H., Hernalsteens, J.P., van Montagu, M., Schell, J. (1982) Genetic identification of functions of TL-DNA transcripts in octopine crown galls. EMBO J. 1, 147–152
Lichfield, M.M. (1967) The automated analysis of nitrite and nitrate in blood. Analyst 92, 132–136
Marschner, H. (1984) General introduction to the mineral nutrition of plants. In: Encyclopedia of plant physiology, N.S., vol. 15A: Inorganic plant nutrition, pp. 22–60, Läuchli, A., Bieleski, R.L., eds. Springer, Berlin Heidelberg New York Tokyo
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Ooms, G., Bains, A., Burrell, M.M., Karp, A., Twell, D., Wilcox, E. (1985b) Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium. Theor. Appl. Genet. 17, 325–329
Ooms, G., Bossen, M.E., Burrell, M.M., Karp, A. (1986) Genetic manipulation in potato using Agrobacterium rhizogenes. Potato Res. (in press)
Ooms, G., Hooykaas, P.J.J., Moolenaar, G., Schilperoort, R.A. (1981) Crown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti-plasmids: analysis of T-DNA functions. Gene 14, 33–50
Ooms, G., Karp, A., Burrell, M.M., Twell, D., Roberts, J. (1985a) Genetic modification of potato development using Ri T-DNA. Theor. Appl. Genet. 70, 440–446
Pomponi, M., Spano, L., Sabbadini, M.G., Costantino, P. (1983) Restriction endonuclease mapping of the root-inducing plasmid of Agrobacterium rhizogenes 1855. Plasmid 10, 119–129
Riker, A.J., Banfield, W.K., Wright, W.H., Keith, G.W., Sagen, H.E. (1930) Studies on infectious hairy-root of nursery apple trees. J. Agric. Res. 41, 507–540
Schroder, G., Waffenschmidt, S., Weiler, E.W., Schroder, J. (1984) The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur. J. Biochem. 138, 387–391
Seemann, J.R., Critchley, C. (1985) Effects of salt stress on the growth, ion content, stomatal behaviour and photosynthetic capacity of a salt-sensitive species, Phaseolus vulgaris L. Planta 164, 151–162
Slightom, J.L., Durant-Tardif, Jouanin, L., Tepfer D. (1986) Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid: identification of open-reading frames (in press)
Smith, E.F., Townsend, C.O. (1907) A plant-tumor of bacterial origin. Science 25, 671–673
Snedecor, G.W., Cochran, W.G. (1967) Statistical methods. 6th ed. Iowa State Univ. Press, Iowa USA
Spano, L., Pomponi, M., Costantino, P., Van Slogteren, G.M.S., Tempe, J. (1982) Identification of T-DNA in the root-inducing plasmid of the agropine type Agrobacterium rhizogenes 1855. Plant Mol. Biol. 1, 291–300
Tepfer, D. (1984) Transformation of several species of higher-plants by Agrobacterium rhizogenes — sexual transmission of the transformed genotype and phenotype. Cell 37, 959–967
Thomashow, L.S., Reeves, S., Thomashow, M.F. (1984) Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTi A6 encodes an enzyme that catalyzes synthesis of indole aetic acid. Proc. Natl. Acad. Sci. USA 81, 5071–5075
Tomos, A.D., Leigh, R.A., Shaw, C.A., Wyn Jones, R.G. (1984) A comparison of methods for measuring turgor pressures and osmotic pressures of cells of red beet storage tissue. J. Exp. Bot. 35, 1675–1683
Turner, N.C. (1981) Techniques and experimental approaches for the measurement of plant water status. Plant Soil 58, 339–366
White, F.F., Ghidossi, G., Gordon, M.P., Nester, E.W. (1982) Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc. Natl. Acad. Sci. USA 79, 3193–3197
White, F.F., Garfinkel, D.J., Huffman, G.A., Gordon, M.P., Nester, E.W. (1983) Sequences homologous to Agrobacterium rhizogenes T-DNA in the genomes of uninfected plants. Nature 301, 348–350
White, F.F., Taylor, B.H., Huffmann, G.A., Gordon, M.P., Nester, E.W. (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J. Bacteriol. 164, 33–44
Willmitzer, L., Sanchez-Serrano, J., Buschfield, E., Schell, J. (1982) DNA from Agrobacterium rhizogenes is transferred to and expressed in axenic hairy root plant tissues. Mol. Gen. Genet. 186, 16–22
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Ooms, G., Atkinson, J., Bossen, M.E. et al. TL-DNA from Agrobacterium rhizogenes plasmid pRi1855 reduces the osmotic pressure in transformed plants grown in vitro. Planta 168, 106–112 (1986). https://doi.org/10.1007/BF00407016
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DOI: https://doi.org/10.1007/BF00407016