Abstract
Isoelectrofocusing, product analysis, thermal denaturation studies and affinity chromatography on cycloheptaamylose-Sephadex were used to identify the amylolytic enzymes in internodes of deepwater rice (Oryza sativa L.). Amylolytic activity in internodes of deepwater rice consists of α-amylase (sometimes separated into two isoforms) and of β-amylase. During submergence of whole plants, α-amylase activity increases in young, growing internodes, but β-amylase activity declines. Although non-growing, mature internodes contain higher levels of β-amylase than do the elongating younger internodes, the effect of submergence on amylase activities in both tissues follows the same trend. Submergence, gibberellic acid (GA3) and ethylene all promote α-amylase activity in growing and non-growing internodes of excised deepwater-rice stem sections. Inhibitor studies showed that submergence and ethylene promote α-amylase activity in the absence of endogenous gibberellin (GA), and GA3 enhances α-amylase activity when ethylene action is inhibited. Therefore, ethylene and GA appear to increase α-amylase activity independently of each other. Enhanced α-amylase activities are probably responsible for the mobilization of carbohydrates which are needed to support internode elongation during submergence of deepwater rice.
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Abbreviations
- CHA:
-
cycloheptaamylose
- GA3 :
-
gibberellic acid
- NBD:
-
2,5-norbornadiene
- TCY:
-
tetcyclacis
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Smith, M.A., Jacobsen, J.V. & Kende, H. Amylase activity and growth in internodes of deepwater rice. Planta 172, 114–120 (1987). https://doi.org/10.1007/BF00403036
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DOI: https://doi.org/10.1007/BF00403036