Abstract
The relationships between photoinhibition and photoprotection in high and low-light-grown Ulva were examined by a combination of chlorophyll-fluorescence-monitoring techniques. Tissues were exposed to a computer-controlled sequence of 5-min exposures to red light, followed by 5-min darkness, with stepwise increases in photon flux. Coefficients of chlorophyll fluorescence quenching (1−qP and NPQ) were calculated following a saturating pulse of white light near the end of each 5-min light treatment. Dark-adapted chlorophyll fluorescence parameters (F0 and FV/FM) were calculated from a saturating pulse at the end of each 5-min dark period. Low-light-grown Ulva showed consistently higher 1−qP, i.e. higher reduction status of Q (high primary acceptor of photosystem II), and lower capacity for nonphotochemical quenching (NPQ) at saturating light than did high-light-grown plants. Consequently, low-light plants rapidly displayed photoinhibitory damage (increased F0) at light saturation in seawater. Removal of dissolved inorganic carbon from seawater also led to photoinhibitory damage of high-light-grown Ulva at light saturation, and addition of saturating amounts of dissolved inorganic carbon protected low-light-grown plants against photoinhibitory damage. A large part of NPQ was abolished by treatment with 3 mM dithiothreitol and the processes so inhibited were evidently photoprotective, because dithiothreitol treatment accelerated photoinhibitory damage in both low- and high-light-grown Ulva. The extent of photoinhibitory damage in Ulva was exacerbated by treatment with chloramphenicol (1 mM) without much effect on chlorophyll-quenching parameters, evidently because this inhibitor of chloroplast protein synthesis reduced the rate of repair processes.
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Abbreviations
- CAP:
-
chloramphenicol
- DIC:
-
dissolved inorganic carbon in seawater (i.e. CO2 + HCO -3 +CO 2-3 )
- DTT:
-
dithiothreitol
- 1-qP :
-
reduction status of Q, the primary acceptor of PSII, measured as described
- FM, F0, FV :
-
dark-adapted maximum, minimum and variable fluorescence, respectively
- NPQ:
-
nonphoto-chemical fluorescence quenching, measured as described
- PFD:
-
photon flux density
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The helpful comments of Barbara Demmig-Adams, William W. Adams III, Ulrich Schreiber, Agu Laisk and Fred Chow on early drafts of this manuscript are appreciated. The research was made possible by a grant from the U.S. National Science Foundation, Biological Oceanography OCE 8812157 to C.B.O. and J.R. and a NSF-CNRS exchange fellowship to G.L.
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Osmond, C.B., Ramus, J., Levavasseur, G. et al. Fluorescence quenching during photosynthesis and photoinhibition of Ulva rotundata blid.. Planta 190, 97–106 (1993). https://doi.org/10.1007/BF00195680
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DOI: https://doi.org/10.1007/BF00195680